绒毡层细胞延迟降解导致紫宸紫薇雄性不育

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-07-01 DOI:10.1016/j.scienta.2025.114261

Ruoyong Yin , Baojin Zhang , Shuaijie Lu , Xugao Sun , Xiaodi Liu , Ruchun Xi , Xiaomei Deng

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引用次数: 0

摘要

绒毡层和四分体胼胝质的降解对小孢子的发育和成熟起着至关重要的作用。紫宸紫薇是一种花粉不育、连续开花的紫薇新品种。在本研究中，我们发现紫柑花的花药发育异常，绒毡层细胞降解延迟。细胞学观察发现，绒毡层细胞的降解在四分体形成后延迟。组织化学表明胼胝质一直持续到单细胞早期。RT-qPCR分析表明，胼胝质合成酶基因在小孢子细胞期表达异常，胼胝质降解酶相关基因在四分体期表达异常低。RT-qPCR结果显示，早在小孢子母细胞时期，l.s speciosa ‘紫袍’的胼胝质合成酶基因表达就已经异常，胼胝质降解酶相关基因在四分体时期表达最低。综上所述，我们推测绒毡层细胞的延迟降解抑制了四分体阶段胼胝质降解酶的释放，进而影响了胼胝质降解和小孢子适时释放，最终导致L. speciosa ‘Zichan’小孢子失败和雄性不育。

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Delayed degradation of tapetal cells causes the male sterility in Lagerstroemia speciosa 'Zichan'

The degradation of tapetum and tetrad callose plays crucial roles in the development and maturation of microspores. Lagerstroemia speciosa 'Zichan' is a new cultivar of Lagerstroemia speciosa characterized by pollen sterility and continuous flowering. In this study, abnormal anther development with the delayed degradation of tapetal cells was identified in L. speciosa 'Zichan'. Cytological observation revealed that the degradation of the tapetal cells was delayed after tetrad formation. Histochemistry showed that callose persisted until the early unicellular stage. RT-qPCR analysis indicated that the expression of callose synthase genes was already abnormal at the microsporocyte stage, and the expression level of callose-degrading enzyme-related genes was abnormally low at the tetrad stage. RT-qPCR indicated that as early as the microsporocyte stage, the expression of callose synthase genes in L. speciosa 'Zichan' was already abnormal, and the expression of callose-degrading enzyme-related genes was lowest at the tetrad stage. Taken together, we hypothesize that the delayed degradation of tapetal cells inhibits the release of callose-degrading enzyme at the tetrad stage, further affecting the degradation of callose and microspore release at the right time, finally leading to microspore failure and male sterility in L. speciosa 'Zichan'.

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.