鸟尾草叶片外植体高效体细胞胚胎发生研究

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-07-01 DOI:10.1016/j.scienta.2025.114265

Mu Ze , Binyue Zhang , Haoyu Ma , Yanbo Guo , Yao Yang , Ming Dong , Lijuan Zou

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引用次数: 0

摘要

尾状鸟galum caudatum Ait （O.尾状鸟）是一种极具观赏价值的药用植物，在生物勘探领域具有显著的商业潜力。本研究利用叶片外植体在添加植物生长调节剂（pgr）的Murashige和Skoog （MS）培养基上建立了直接和间接体细胞胚胎发生（SE）途径。1.0-3.0 mg L - 1生长素（α-萘基乙酸（NAA）、吲哚-3-丁酸（IBA）和二氯苯氧乙酸（2,4- d））和四种细胞分裂素（噻唑脲（TDZ）、6-苄基腺嘌呤（BA）、动素（Kin）和玉米素（ZT））直接或直接处理的叶片外植体产生体细胞胚胎。IBA作为唯一的PGR，在84.7%的频率下，每个外植体产生16.3±2.5个胚。2,4- d处理通过间接途径产生胚性愈伤组织，随后的SE诱导峰值为43.6%（8.6±1.5个胚/外植体）。细胞分裂素-生长素的协同作用显著提高了再生效率：0.3 mg L - 1 NAA与2.0 mg L - 1 TDZ或BA可以达到最大的SE诱导（分别为100%和98.9%）和胚胎产生（每个外植体24.7±1.8和22.5±3.1）。赤霉素酸3 （GA3）促进体胚成熟。硒源植株在温室驯化期间的成活率为98.0%。本研究通过直接和间接的SE途径建立了可靠和有效的再生策略，实现了这种具有商业价值的物种的可扩展繁殖、基因工程和种质保护。

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High-efficiency somatic embryogenesis from leaf explants of Ornithogalum caudatum Ait

Ornithogalum caudatum Ait (O. caudatum), a highly valued ornamental and medicinal species, holds notable commercial potential for bioprospecting industries. This study established both direct and indirect somatic embryogenesis (SE) pathways using leaf explants on Murashige and Skoog (MS) medium supplemented with plant growth regulators (PGRs). Leaf explants treated with 1.0–3.0 mg L⁻¹ auxin (α-naphthylacetic acid (NAA), indole-3-butyric acid (IBA) and dichlorophenoxyacetic acid (2,4-D)), and four types cytokinins (thidiazuron (TDZ), 6-benzyladenine (BA), kinetin (Kin), and zeatin (ZT)) directly or direct produced somatic embryos. IBA as the sole PGR optimally induced direct SE, yielding 16.3 ± 2.5 embryos per explant at 84.7 % frequency. Treatment with 2,4-D generated embryogenic calli via an indirect pathway, with subsequent SE induction peaking at 43.6 % (8.6 ± 1.5 embryos/explant). Cytokinin - auxin synergism markedly enhanced regeneration efficiency: 0.3 mg L⁻¹ NAA with 2.0 mg L⁻¹ TDZ or BA achieved maximal SE induction (100 % and 98.9 %, respectively) and embryo production (24.7 ± 1.8 and 22.5 ± 3.1 per explant). Gibberellic acid 3 (GA₃) promoted somatic embryo maturation. SE-derived plantlets exhibited 98.0 % survival during greenhouse acclimatization. This work established a trustworthy and efficient strategy for regeneration through direct and indirect SE pathways, enabling scalable propagation, genetic engineering, and germplasm conservation of this commercially valuable species.

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.