不同粘度海藻酸钠对大鼠脂质代谢和肠道微生物群的性别特异性影响。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-10-01 Epub Date: 2025-07-02 DOI:10.1002/jsfa.70018

Zhi Chen, Mengmei Ni, Xiaoya Wang, Yufei Liu, Jiaojiao Shi, Wanqing Guo, Xiaomeng Li, Lishi Zhang, Ning Lin, Jinyao Chen

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引用次数: 0

摘要

背景：海藻酸钠（SA）是一种膳食纤维，已知可影响脂质代谢和肠道微生物群。然而，其长期影响粘度/剂量和性别特异性差异仍未得到充分探讨。本实验研究了不同粘度和剂量的SA对120只雄性和雌性Sprague-Dawley大鼠90天内脂质代谢和肠道微生物群的影响。结果：低粘度高剂量（Lv-SA-H, 250 ~ 350 mPa s, 8.00 g kg-1）和高粘度（lv -SA, 600 ~ 800 mPa s, 8.00 g kg-1） SA均能显著降低体脂。低黏度低剂量SA （Lv-SA-L, 250 ~ 350 mPa s, 4.00 g kg-1）、Lv-SA-H和Hv-SA处理均降低血清甘油三酯水平，升高血清总胆汁酸水平。SA还提高了粪便中乙酸和丁酸的水平，通过促进23-去甲氧胆酸（NorDCA）的排泄来调节粪便胆汁酸谱，并增加了3β-熊去氧胆酸（β-UDCA）的水平。16S rDNA基因扩增子测序结果显示，添加Lv-SA-H增加了Akkermansia和Bacteroides的相对丰度，而Lv-SA-L、Lv-SA-H和Hv-SA均降低了黄酮因子的丰度。雌性大鼠的NorDCA水平与黄酮因子的相对丰度呈正相关，雄性大鼠的β-UDCA水平与拟杆菌的相对丰度呈正相关。结论：我们的研究表明，SA通过调节肠道菌群来调节脂质代谢，从而影响短链脂肪酸和胆汁酸的谱。这些影响因性别和黏度而异，强调了在未来营养干预中准确性的重要性。©2025化学工业协会。

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The sex-specific effects of sodium alginate with varying viscosities on lipid metabolism and gut microbiota in rats.

Background: Sodium alginate (SA), a dietary fiber, is known to influence lipid metabolism and gut microbiota. However, its long-term effects of viscosity/dose and sex-specific differences remain underexplored. This study investigates the impact of SA with different viscosities and doses on lipid metabolism and gut microbiota in 120 male and female Sprague-Dawley rats over 90 days.

Results: Both low-viscosity high-dose (Lv-SA-H, 250-350 mPa s, 8.00 g kg^-1) and high-viscosity (Hv-SA, 600-800 mPa s, 8.00 g kg^-1) SA significantly reduced body fat. Three treatments - low-viscosity low-dose SA (Lv-SA-L, 250-350 mPa s, 4.00 g kg^-1), Lv-SA-H and Hv-SA - decreased serum triglyceride levels and increased serum total bile acid levels in serum. SA also elevated the levels of acetic and butyric acids in feces, modulated the fecal bile acids profile by promoting the excretion of 23-nordeoxycholic acid (NorDCA) and increased the levels of 3β-ursodeoxycholic acid (β-UDCA). 16S rDNA gene amplicon sequencing revealed that Lv-SA-H supplementation increased the relative abundance of Akkermansia and Bacteroides, while Lv-SA-L, Lv-SA-H and Hv-SA all reduced the abundance of Flavonifractor. Furthermore, a positive correlation was observed between NorDCA levels and the relative abundance of Flavonifractor in female rats, while β-UDCA levels were positively correlated with the relative abundance of Bacteroides in male rats.

Conclusion: Our study suggests that SA regulates lipid metabolism by modulating the gut microbiota, which in turn affects the profiles of short-chain fatty acids and bile acids. These effects vary by sex and viscosity, emphasizing the importance of precision in future nutritional interventions. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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