低温下木瓜蛋白质组学分析揭示了细胞壁代谢对低温损伤的适应。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-07-02 DOI:10.1002/jsfa.70032

Song Wu, Lijin Huang, Lisha Zhu, Lanhuan Meng, Hanyue Yang, Yaxin Tan, Yonggui Pan

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引用次数: 0

摘要

背景：木瓜在低温贮藏过程中容易受到冷害（CI）。然而，值得注意的是，保存在1°C的木瓜表现出较轻的CI症状，可能归因于细胞壁代谢的改变。为了揭示木瓜这种异常CI反应背后的细胞壁代谢途径，我们的研究对在1和6°C下储存12、24和36天的水果进行了蛋白质组学分析。结果：对差异表达蛋白（DEPs）的分析显示，温度是表达差异的主要驱动因素，在三个对照组（1-12对6-12,1-24对6-24,1-36对6-36）中鉴定出122个DEPs（115个下调，7个上调）。富集与碳水化合物代谢、细胞壁生物发生和β-半乳糖苷酶活性有关，其中半乳糖代谢是关键途径。基于蛋白质组学数据的进一步筛选确定了20个与细胞壁代谢特异性相关的dep。蛋白质-蛋白质相互作用分析强调了这些DEPs在木质素合成、生物应激反应和整体细胞壁代谢中的重要作用。结论：1℃保存木瓜可调节细胞壁代谢相关蛋白的表达，抑制细胞壁成分的降解。这反过来又加强了细胞壁的稳定性，维持了组织的完整性，并最终减轻了CI的进展。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Proteome analysis of papaya at low temperatures reveals adaptations in cell wall metabolism in response to chilling injury

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Proteome analysis of papaya at low temperatures reveals adaptations in cell wall metabolism in response to chilling injury

BACKGROUND

Papaya is susceptible to chilling injury (CI) during low-temperature storage. However, it is noteworthy that papaya stored at 1 °C exhibits milder CI symptoms, potentially attributed to alterations in cell wall metabolism. To unravel the cellular wall metabolic pathways underlying this abnormal CI response in papaya, our research conducted proteomic analysis on fruit stored at 1 and 6 °C for 12, 24 and 36 days.

RESULTS

Analysis of differentially expressed proteins (DEPs) revealed temperature as the primary driver of expression divergence, with 122 DEPs (115 downregulated, 7 upregulated) identified across three comparison groups (1–12 versus 6–12, 1–24 versus 6–24, 1–36 versus 6–36). Enrichment showed significant involvement in carbohydrate metabolism, cell wall biogenesis and β-galactosidase activity, with galactose metabolism as a key pathway. Further screening based on proteomic data identified 20 DEPs specifically linked to cell wall metabolism. Protein–protein interaction analyses highlighted the crucial roles of these DEPs in lignin synthesis, responses to biological stresses and overall cell wall metabolism.

CONCLUSION

The findings reveal that papaya stored at 1 °C modulates the expression of proteins related to cell wall metabolism, inhibiting the degradation of cell wall components. This, in turn, fortifies cell wall stability, maintains tissue integrity and ultimately mitigates the progression of CI. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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