慢性缺氧妊娠新生儿皮层氧化还原失衡与神经元凋亡相关

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Esteban G Figueroa, Rodrigo L Castillo, Adolfo A Paz, Matías Monsalves-Alvarez, Francisca Salas-Pérez, Ximena Calle, Tamara A Jiménez, Emilio A Herrera, Alejandro Gonzaléz-Candia
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引用次数: 0

摘要

妊娠期慢性缺氧影响产前发育,导致胎儿生长受限(FGR),即胎儿未能达到其遗传生长潜能。产后脑组织缺氧可引起氧化还原失衡和线粒体功能障碍,从而增加神经元死亡。然而,这些数据不能推断出产前缺氧。因此,本研究旨在探讨妊娠期缺氧对豚鼠前额叶皮层氧化还原平衡及凋亡细胞死亡机制的影响。本研究选用10只豚鼠(Cavia porcellus)妊娠坝;5个在常氧条件下妊娠(Nx;3名男婴,2名女婴)和5名在慢性低压缺氧(Hx;两只刚出生的雄性和三只雌性)。从妊娠第20 ~ 65天(足月~ 70天)采用超声检查监测妊娠情况。出生后,对幼鼠实施安乐死,收集胎儿大脑进行细胞氧化还原测定、线粒体酶表达和细胞凋亡测定。妊娠缺氧诱导抗氧化酶和促氧化酶表达失衡,导致氧化应激增加。此外,观察到新生儿前额皮质细胞色素I和III的表达和神经元密度下降。最后,通过TUNEL检测,慢性缺氧妊娠的新生儿脑组织中DNA片段化增加。我们的研究结果表明,妊娠缺氧与新生儿氧化应激和神经元死亡有关,这可能导致成年后神经元功能障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Redox Imbalance Is Associated with Neuronal Apoptosis in the Cortex of Neonates Gestated Under Chronic Hypoxia.

Gestational chronic hypoxia impacts prenatal development, leading to fetal growth restriction (FGR), defined as the fetus's failure to reach its genetic growth potential. Postnatal hypoxia in the cerebral tissue can induce a redox imbalance and mitochondrial dysfunction, consequently increasing neuronal death. However, these data cannot necessarily be extrapolated to prenatal hypoxia. In this regard, this study aims to describe the effect of gestational hypoxia on redox balance and apoptosis cell death mechanisms in the prefrontal cortex of guinea pigs. Ten Guinea pig (Cavia porcellus) pregnant dams were utilized in this study; five gestated in normoxia (Nx; three newborn males, and two females) and five gestated under chronic hypobaric hypoxia (Hx; two newborn males, and three females). We monitored the pregnancies by ultrasound examinations from gestational days 20 to 65 (term ~ 70). At birth, pups were euthanized, and the fetal brain was collected for cellular redox measurement, mitochondrial enzyme expression, and apoptosis assay. Gestation under hypoxia induced an imbalance in the expression of anti- and pro-oxidant enzymes, resulting in increased oxidative stress. Additionally, a decrease in cytochrome I and III expression and neuronal density in the neonatal prefrontal cortex was observed. Finally, DNA fragmentation was increased by the TUNEL assay in the brain tissue of newborns gestated under chronic hypoxia. Our findings demonstrate the association of gestational hypoxia with oxidative stress and neuronal death in newborns, which may predispose to neuronal dysfunction in adulthood.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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