具有潜在老年治疗作用的有前途的膳食补充剂:酶解大麻籽饼粉和大麻籽蛋白浓缩物的水提取物。

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Anthea Miller, Inga Kwiecień, Marek Bednarski, Małgorzata Zygmunt, Jacek Sapa, Mateusz Sablik, Giorgia Pia Lombardo, Concetta Condurso, Maria Merlino, Magdalena Kotańska
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引用次数: 0

摘要

在本研究中,大麻籽油加工的主要副产物-大麻籽饼粉和大麻籽浓缩蛋白-分别或联合使用蛋白酶和糖酶进行酶解。通过评估水解和未经处理的大麻副产品的水提取物的抗氧化和抗炎特性,评估了这些酶处理在释放生物活性化合物方面的有效性。目的是探索其潜在的老年治疗特性,并促进其作为膳食补充剂的应用。利用高效液相色谱法对黄酮类化合物、酚酸、儿茶素等次生代谢产物进行分析。用分光光度法测定总酚、类黄酮和蛋白质含量。清除活性(2,2-二苯基-1-苦味酰肼清除实验(DPPH实验))、抗氧化能力(铁还原抗氧化能力实验(FRAP实验))和脂质过氧化还原活性(硫代巴比妥酸活性物质分析)通过体外实验进行评估。通过溶血抑制来评估可能的抗炎作用。还确定了提取物对暴露于果糖诱导的白蛋白糖基化的影响。为了评估提取物的毒性,采用斑马鱼幼虫模型。所有提取物均含有大量的酚类化合物、黄酮类化合物和蛋白质,并表现出显著的降低脂质过氧化和稳定红细胞膜的活性。然而,它们没有显著影响蛋白糖基化(糖基化抑制仅在15-40%的范围内)。我们的研究表明,从大麻籽油加工过程的副产品中提取的水提取物具有巨大的促进健康的潜力,包括延缓衰老,这些水提取物大量可用,可以作为有价值的补充剂,以支持包括人类在内的动物的健康,而不是作为油生产的废物丢弃。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Promising Dietary Supplements with Potential Senotherapeutic Effects: Aqueous Extracts from Enzymatically Hydrolysed Hemp Seed Cake Flour and Hemp Seed Protein Concentrate.

In the present study, the primary by-products of the hemp-seed oil process-hemp seed cake flour and hemp seed protein concentrate-underwent enzymatic hydrolysis using proteases and carbohydrases, either individually or in combination. The effectiveness of these enzymatic treatments in releasing bioactive compounds was evaluated by assessing the antioxidant and anti-inflammatory properties of the aqueous extracts of both hydrolysed and untreated hemp by-products. The aim was to explore their potential senotherapeutic properties and promote their application as dietary supplements. Secondary metabolites such as flavonoids, phenolic acids, and catechins were analysed using high-performance liquid chromatography. Total phenolic, flavonoid, and protein contents were determined using spectrophotometric methods. Scavenging activity (2,2-Diphenyl-1-picrylhydrazyl scavenging assay (DPPH assay)), antioxidant power (Ferric reducing antioxidant power assay (FRAP assay)), and lipid peroxidation-reducing activity (thiobarbituric acid-reactive substance analysis) were assessed through in vitro assays. Possible anti-inflammatory effects were evaluated by assessing haemolysis inhibition. The impact of extracts on albumin glycation induced by exposure to fructose was also determined. To assess the toxicity of extracts, a zebrafish larvae model was employed. All extracts contained significant amounts of phenolic compounds, flavonoids, and proteins, and they exhibited notable activities in reducing lipid peroxidation and stabilising erythrocyte cell membranes. However, they did not significantly influence protein glycation (the glycation inhibition was only in the range of 15-40%). Our research demonstrates the substantial health-promoting potential, including senescence delay, of aqueous extracts from by-products of the hemp-seed oil process, which are available in large quantities and can serve as valuable supplements to support the health of animals, including humans, rather than being discarded as waste from oil production.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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