非洲李树皮、Leea indica和Paullinia pinata叶提取物的植物化学分析及其抗氧化活性。

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Md Rezaul Karim, Karl E Miletti-Gonzalez, Alberta N A Aryee, Samuel A Besong
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引用次数: 0

摘要

研究了喀麦隆非洲李、印度李和山楂叶的植物化学特征和抗氧化活性。非洲白杨树皮、印度白杨和桄榔子叶的纯甲醇提取率分别为11.9%、11.1%和10.8%。非洲皮、籼米和山楂叶的总酚含量分别为189.0±16.93、163.6±14.73和114.6±10.38 mg GAE/g,总黄酮含量分别为43.25±6.43、28.31±4.44和19.75±4.03 mg RU/g。采用DPPH、ABTS、FRAP等方法评价其抗氧化活性。非洲栎、籼树和山楂叶DPPH的IC50分别为109.5±13.2、132.1±18.7和156.1±21.9µg/mL, ABTS的IC50分别为98.1±4.8、101.3±12.1和133.9±16.0µg/mL。同一序列的FRAP值分别为61.1±1.5、50.5±1.5和43.4±2.1µMFe2+/g。通过傅里叶变换红外分析,鉴定了相应植物化学物质的官能团,包括烷烃、烯烃、脂肪醚、酯、胺、α、β-不饱和酯、醇、酚、羧酸和脂肪酮。本研究鉴定和定量的酚酸分别为甲基-4-羟基苯甲酸、咖啡酸、原儿茶酸和对香豆酸,采用高效液相色谱法鉴定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical Analysis and Antioxidant Activities of Prunus africana Bark, Leea indica and Paullinia pinnata Leaf Extracts.

The phytochemical profile and antioxidant activities of Prunus africana bark, Leea indica and Paullinia pinnata leaves from Cameroon were investigated in this study. The yields of pure methanolic extraction were 11.9%, 11.1% and 10.8% in P. africana bark, L. indica and P. pinnata leaves, respectively. The total phenolic content was 189.0 ± 16.93, 163.6 ± 14.73 and 114.6 ± 10.38 mg GAE/g and total flavonoid content was 43.25 ± 6.43, 28.31 ± 4.44, and 19.75 ± 4.03 mg RU/g in P. africana bark, L. indica and P. pinnata leaves, respectively. The antioxidant activities of the plants were evaluated by DPPH, ABTS and FRAP assays. The IC50 evaluated in P. africana bark, L. indica and P. pinnata leaves was 109.5 ± 13.2, 132.1 ± 18.7 and 156.1 ± 21.9 µg/mL for DPPH and 98.1 ± 4.8, 101.3 ± 12.1 and 133.9 ± 16.0 µg /mL for ABTS assay. The FRAP value was 61.1 ± 1.5, 50.5 ± 1.5 and 43.4 ± 2.1 µMFe2+/g in the same sequence. The functional groups for the corresponding phytochemicals, including alkane, alkene, aliphatic ether, ester, amine, α, β-unsaturated ester, alcohol, phenol, carboxylic acid, and aliphatic ketone, were identified through fourier-transform infrared analysis. The identified and quantified phenolic acids in this study were methyl-4-hydroxybenzoic, caffeic, protocatechuic and p-coumaric acid, identified using high-performance liquid chromatography.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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