综合转录组和代谢组分析揭示了不同碳酸盐胁迫下水稻幼苗根系复杂的氧化损伤机制。

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yang Cao, Fei Hao, Jingpeng Li, Bolun Zhang, Zeming Li, Tiantian Liu, Yan Gao, Xuguang Niu, Xiaohu Liu, Hui Zhang, Lijuan Yang
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引用次数: 0

摘要

碱胁迫是严重影响水稻生长和产量的主要威胁之一。然而,碱盐碱地主要成分碳酸钠(Na2CO3)和碳酸氢钠(NaHCO3)对水稻幼苗的危害差异尚不清楚。本研究探讨了不同碳酸盐胁迫(Na2CO3和NaHCO3)对水稻幼苗生长、根系损伤、生理反应和分子变化的影响。通过这些不同的碳酸盐处理,我们观察到两种胁迫都显著抑制了水稻的生长。而Na2CO3处理的抑制作用更为明显。与NaHCO3处理相比,Na2CO3胁迫对根细胞膜的损伤更为严重,导致根系活力大幅下降。活性氧(ROS)和丙二醛(MDA)含量显著升高,表明Na2CO3诱导的氧化损伤更为严重。转录组学和代谢组学分析显示,Na2CO3治疗组差异表达基因(DEGs)和差异表达代谢物(dem)数量更多。综合分析和验证表明,在Na2CO3胁迫下,与生长素、抗坏血酸、类黄酮和谷胱甘肽代谢相关的途径特别丰富。这些结果表明,Na2CO3胁迫可能会干扰生长素信号通路,并对内源抗氧化系统产生更深远的影响,在多个水平上影响水稻生长。综上所述,本研究强调了Na2CO3和Na2CO3胁迫对水稻幼苗生长、生理和分子过程的差异影响,特别是氧化损伤和抗氧化反应。研究结果为提高水稻耐碱性和制定盐碱地水稻合理栽培策略提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Integrated Transcriptome and Metabolome Analyses Reveal Complex Oxidative Damage Mechanisms in Rice Seedling Roots Under Different Carbonate Stresses.

Alkaline stress (AS) is one of the major threats that severely affects rice growth and grain yield. However, the differences in the damage caused by the main components of soda saline-alkali land, sodium carbonate (Na2CO3), and sodium bicarbonate (NaHCO3) to rice seedlings are still unclear. This study explored the effects of different carbonate stresses (Na2CO3 and NaHCO3) on rice seedling growth, root damage, physiological responses, and molecular changes. By administering equivalent concentrations of sodium ions through these different carbonate treatments, we observed that both stresses significantly inhibited rice growth. However, the inhibitory effect was more pronounced under the Na2CO3 treatment. Compared with the NaHCO3 treatment, Na2CO3 stress caused more severe damage to root cell membranes and led to a substantial decline in root vigor. Moreover, the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were markedly increased, indicating that Na2CO3 induces more severe oxidative damage. Transcriptomic and metabolomic analyses revealed a greater number of differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) in the Na2CO3 treatment group. The integrative analysis and validation demonstrated that pathways related to auxin, ascorbate, flavonoids, and glutathione metabolism were particularly enriched under Na2CO3 stress. These findings suggest that Na2CO3 stress may interfere with auxin signaling pathways and exerts a more profound impact on endogenous antioxidant systems, affecting rice growth at multiple levels. In summary, this research highlights the differential impacts of Na2CO3 and Na2CO3 stresses on rice seedling growth, physiology, and molecular processes, particularly oxidative damage and antioxidant responses. The insights gained provide a valuable theoretical foundation for enhancing rice alkali tolerance and developing strategies for the rational cultivation of rice in saline-alkaline soils.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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