精氨酸介导的大口黑鲈肝脏免疫调节和抗氧化防御:在诺卡菌感染期间代谢重塑的多组学见解。

IF 6.6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yu-Long Sun, Shuai-Liang Zhang, Feng-Feng Zhou, Yuan-Xin Qian, Yang He, Run-Zhe Zhang, Fen Dong, Qiang Chen, Han-Ying Xu, Ji-Teng Wang, Yu-Ting Deng, Tao Han
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引用次数: 0

摘要

鱼的肝脏是一个重要的代谢器官,也起着免疫调节作用。本研究建立了注射感染诺卡菌的大口黑鲈(Micropterus salmoides)模型,探讨其肝脏的免疫和抗氧化功能。结果显示,梭状奈瑟菌感染引起肝脏严重的病理改变,包括细胞坏死、肉芽肿形成和白细胞浸润。感染后2 d,肝脏免疫相关基因mRNA表达水平显著升高。此外,转录组学和代谢组学的联合分析表明,瑟雷菌感染显著影响肝脏代谢,包括谷胱甘肽代谢、精氨酸和脯氨酸代谢、花生四烯酸代谢以及淀粉和蔗糖代谢。此外,研究人员还发现了多个关键生物标志物,包括精氨酸、谷胱甘肽、gpx、GST、PLA2G、GAA和PYG,参与了对seriolae感染的调节。为了进一步阐明精氨酸对肝脏免疫和抗氧化过程的调节作用,我们分离培养了原代肝细胞。结果表明,补充精氨酸可显著降低lps诱导的凋亡相关基因(bax、cas3、cas8和cas9)在24 h内的表达量高达50%,同时可使抗氧化基因(gpx、GST)的表达量增加高达700%。本研究通过分析梭状芽孢杆菌感染后肝脏的代谢变化和免疫反应,结合体外实验,阐明了精氨酸的抗凋亡和抗氧化作用。揭示鱼类肝脏免疫应答机制,为利用营养策略改善鱼类健康奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Arginine-Mediated Liver Immune Regulation and Antioxidant Defense in Largemouth Bass (Micropterus salmoides): Multi-Omics Insights into Metabolic Remodeling During Nocardia seriolae Infection.

The liver of fish is an essential metabolic organ that also serves an immune regulatory role. In this study, we constructed a model of largemouth bass (Micropterus salmoides) infected with Nocardia seriolae by injection to explore the immune and antioxidant functions of the liver. The results showed that N. seriolae infection caused severe pathological changes in the liver, including cell necrosis, granuloma formation, and leukocyte infiltration. The level of mRNA expression of immune-related genes in the liver was significantly increased 2 days post-infection. Moreover, the combined analysis of transcriptome and metabolome showed that N. seriolae infection markedly affected liver metabolism, including glutathione metabolism, arginine and proline metabolism, arachidonic acid metabolism, as well as starch and sucrose metabolism. Additionally, multiple key biomarkers were identified as involved in regulating responses to N. seriolae infection, including arginine, glutathione, gpx, GST, PLA2G, GAA, and PYG. To further elucidate the regulatory effects of arginine on the immune and antioxidant processes in the liver, primary hepatocytes were isolated and cultured. The results demonstrated that arginine supplementation significantly reduced the expression of LPS-induced apoptosis-related genes (bax, cas3, cas8, and cas9) by up to 50% while increasing the expression of antioxidant genes (gpx, GST) by up to 700% at 24 h. Through the analysis of metabolic changes and immune responses in the liver following N. seriolae infection, combined with in-vitro experiments, this study elucidated the anti-apoptotic and antioxidant effects of arginine, revealing the immune response mechanisms in fish liver and laying the groundwork for using nutritional strategies to improve fish health.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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