Melissa Zannini, Maddalena De Angeli, Angela Conte, Vincenzo Minischetti, Domenico D'Arca, Davide Tagliazucchi, Alice Cattivelli
{"title":"肠道微生物代谢产物酚类化合物通过触发细胞凋亡和细胞周期阻滞抑制结肠癌细胞增殖。","authors":"Melissa Zannini, Maddalena De Angeli, Angela Conte, Vincenzo Minischetti, Domenico D'Arca, Davide Tagliazucchi, Alice Cattivelli","doi":"10.1039/d5fo02116b","DOIUrl":null,"url":null,"abstract":"<p><p>Growing evidence shows how diet can influence the onset of colon cancer. In this regard, phenolic compounds, and particularly, their metabolites produced by the gut microbiota, may be responsible for this protective effect. Therefore, in this study, some of the main gut microbial metabolites of phenolic compounds were tested for their anti-proliferative activity against two colon adenocarcinoma cell lines (Caco-2 and SW480). Two compounds, 3',4'-dihydroxyphenylacetic acid and 3-(3',4'-dihydroxyphenyl)propanoic acid, showed IC<sub>50</sub> values towards Caco-2 below the physiological concentrations found in the colon. In addition, other compounds showing low IC<sub>50</sub> values towards Caco-2 were 2,3,4-trihyroxybenzoic acid (gallic acid), 1,2,3-trihydroxybenzene (pyrogallol), and 5-(3',4'-dihydroxyphenyl)-γ-valerolactone. Whereas three flavanone aglycones (naringenin, eriodictyol, and hesperetin) and, again, 2,3,4-trihyroxybenzoic acid, and 1,2,3-trihydroxybenzene were the most active towards SW480. Some compounds exert an anti-proliferative effect by disrupting the cell cycle, others by inducing apoptosis, and others by promoting reactive oxygen species formation. Thus, this study demonstrates that gut microbial metabolites of phenolic compounds can be held responsible for the protective effect against the onset of colon cancer of a diet rich in fruit and vegetables.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.1000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Gut microbial metabolites of phenolic compounds inhibit colon cancer cell proliferation by triggering apoptosis and cell cycle arrest.\",\"authors\":\"Melissa Zannini, Maddalena De Angeli, Angela Conte, Vincenzo Minischetti, Domenico D'Arca, Davide Tagliazucchi, Alice Cattivelli\",\"doi\":\"10.1039/d5fo02116b\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Growing evidence shows how diet can influence the onset of colon cancer. In this regard, phenolic compounds, and particularly, their metabolites produced by the gut microbiota, may be responsible for this protective effect. Therefore, in this study, some of the main gut microbial metabolites of phenolic compounds were tested for their anti-proliferative activity against two colon adenocarcinoma cell lines (Caco-2 and SW480). Two compounds, 3',4'-dihydroxyphenylacetic acid and 3-(3',4'-dihydroxyphenyl)propanoic acid, showed IC<sub>50</sub> values towards Caco-2 below the physiological concentrations found in the colon. In addition, other compounds showing low IC<sub>50</sub> values towards Caco-2 were 2,3,4-trihyroxybenzoic acid (gallic acid), 1,2,3-trihydroxybenzene (pyrogallol), and 5-(3',4'-dihydroxyphenyl)-γ-valerolactone. Whereas three flavanone aglycones (naringenin, eriodictyol, and hesperetin) and, again, 2,3,4-trihyroxybenzoic acid, and 1,2,3-trihydroxybenzene were the most active towards SW480. Some compounds exert an anti-proliferative effect by disrupting the cell cycle, others by inducing apoptosis, and others by promoting reactive oxygen species formation. Thus, this study demonstrates that gut microbial metabolites of phenolic compounds can be held responsible for the protective effect against the onset of colon cancer of a diet rich in fruit and vegetables.</p>\",\"PeriodicalId\":77,\"journal\":{\"name\":\"Food & Function\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":5.1000,\"publicationDate\":\"2025-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food & Function\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1039/d5fo02116b\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food & Function","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1039/d5fo02116b","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Gut microbial metabolites of phenolic compounds inhibit colon cancer cell proliferation by triggering apoptosis and cell cycle arrest.
Growing evidence shows how diet can influence the onset of colon cancer. In this regard, phenolic compounds, and particularly, their metabolites produced by the gut microbiota, may be responsible for this protective effect. Therefore, in this study, some of the main gut microbial metabolites of phenolic compounds were tested for their anti-proliferative activity against two colon adenocarcinoma cell lines (Caco-2 and SW480). Two compounds, 3',4'-dihydroxyphenylacetic acid and 3-(3',4'-dihydroxyphenyl)propanoic acid, showed IC50 values towards Caco-2 below the physiological concentrations found in the colon. In addition, other compounds showing low IC50 values towards Caco-2 were 2,3,4-trihyroxybenzoic acid (gallic acid), 1,2,3-trihydroxybenzene (pyrogallol), and 5-(3',4'-dihydroxyphenyl)-γ-valerolactone. Whereas three flavanone aglycones (naringenin, eriodictyol, and hesperetin) and, again, 2,3,4-trihyroxybenzoic acid, and 1,2,3-trihydroxybenzene were the most active towards SW480. Some compounds exert an anti-proliferative effect by disrupting the cell cycle, others by inducing apoptosis, and others by promoting reactive oxygen species formation. Thus, this study demonstrates that gut microbial metabolites of phenolic compounds can be held responsible for the protective effect against the onset of colon cancer of a diet rich in fruit and vegetables.
期刊介绍:
Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.