肠道微生物代谢产物酚类化合物通过触发细胞凋亡和细胞周期阻滞抑制结肠癌细胞增殖。

IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Food & Function Pub Date : 2025-06-26 DOI:10.1039/d5fo02116b
Melissa Zannini, Maddalena De Angeli, Angela Conte, Vincenzo Minischetti, Domenico D'Arca, Davide Tagliazucchi, Alice Cattivelli
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引用次数: 0

摘要

越来越多的证据表明饮食如何影响结肠癌的发病。在这方面,酚类化合物,特别是由肠道微生物群产生的代谢产物,可能是这种保护作用的原因。因此,在本研究中,我们测试了酚类化合物的一些主要肠道微生物代谢物对两种结肠腺癌细胞系(Caco-2和SW480)的抗增殖活性。3',4'-二羟基苯基乙酸和3-(3',4'-二羟基苯基)丙酸对Caco-2的IC50值低于结肠中发现的生理浓度。此外,其他对caco2具有低IC50值的化合物有2,3,4-三羟基苯甲酸(没食子酸)、1,2,3-三羟基苯(邻苯三酚)和5-(3',4'-二羟基苯基)-γ-戊内酯。而三种黄酮苷元(柚皮素、戊二醇和橙皮素)和2,3,4-三羟基苯甲酸和1,2,3-三羟基苯对SW480的活性最大。一些化合物通过破坏细胞周期发挥抗增殖作用,另一些通过诱导细胞凋亡,还有一些通过促进活性氧的形成。因此,这项研究表明,酚类化合物的肠道微生物代谢物可以对富含水果和蔬菜的饮食预防结肠癌的发生起保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gut microbial metabolites of phenolic compounds inhibit colon cancer cell proliferation by triggering apoptosis and cell cycle arrest.

Growing evidence shows how diet can influence the onset of colon cancer. In this regard, phenolic compounds, and particularly, their metabolites produced by the gut microbiota, may be responsible for this protective effect. Therefore, in this study, some of the main gut microbial metabolites of phenolic compounds were tested for their anti-proliferative activity against two colon adenocarcinoma cell lines (Caco-2 and SW480). Two compounds, 3',4'-dihydroxyphenylacetic acid and 3-(3',4'-dihydroxyphenyl)propanoic acid, showed IC50 values towards Caco-2 below the physiological concentrations found in the colon. In addition, other compounds showing low IC50 values towards Caco-2 were 2,3,4-trihyroxybenzoic acid (gallic acid), 1,2,3-trihydroxybenzene (pyrogallol), and 5-(3',4'-dihydroxyphenyl)-γ-valerolactone. Whereas three flavanone aglycones (naringenin, eriodictyol, and hesperetin) and, again, 2,3,4-trihyroxybenzoic acid, and 1,2,3-trihydroxybenzene were the most active towards SW480. Some compounds exert an anti-proliferative effect by disrupting the cell cycle, others by inducing apoptosis, and others by promoting reactive oxygen species formation. Thus, this study demonstrates that gut microbial metabolites of phenolic compounds can be held responsible for the protective effect against the onset of colon cancer of a diet rich in fruit and vegetables.

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来源期刊
Food & Function
Food & Function BIOCHEMISTRY & MOLECULAR BIOLOGY-FOOD SCIENCE & TECHNOLOGY
CiteScore
10.10
自引率
6.60%
发文量
957
审稿时长
1.8 months
期刊介绍: Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.
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