泰国临床分离的大肠杆菌中mcr-1.1和mcr-3.5的共存

IF 4.3 2区 医学 Q1 INFECTIOUS DISEASES
Panida Nobthai, Sirigade Ruekit, Dutsadee Peerapongpaisarn, Prawet Sukhchat, Brett E Swierczewski, Nattaya Ruamsap, Paphavee Lertsethtakarn
{"title":"泰国临床分离的大肠杆菌中mcr-1.1和mcr-3.5的共存","authors":"Panida Nobthai, Sirigade Ruekit, Dutsadee Peerapongpaisarn, Prawet Sukhchat, Brett E Swierczewski, Nattaya Ruamsap, Paphavee Lertsethtakarn","doi":"10.3390/antibiotics14060596","DOIUrl":null,"url":null,"abstract":"<p><p>The emergence of colistin resistance poses a significant threat to its efficacy as a last-line treatment against multidrug-resistant Gram-negative bacterial infections. In this study, 178 multi-drug resistant (MDR) <i>Escherichia coli</i> isolates collected from clinical samples at Queen Sirikit Naval Hospital, Chonburi, Thailand, were evaluated for colistin resistance. Of these, six were identified as <i>mcr</i> gene carriers, mediating colistin resistance. Specifically, <i>mcr-1</i> was detected in three <i>E. coli</i> isolates, <i>mcr-3</i> was detected in one <i>E. coli</i> isolate, and <i>mcr-1</i> and <i>mcr-3</i> were detected in two <i>E. coli</i> isolates, designated AMR-0220 and AMR-0361. Whole-genome sequencing and bioinformatics analysis revealed that AMR-0220 and AMR-0361 belonged to ST410 and ST617 lineages, respectively. Both isolates carried multiple plasmids, with <i>mcr-1.1</i> located on an IncX4-type plasmid that is closely related to previously reported <i>mcr-1.1</i>-carrying IncX4 plasmids. In contrast, <i>mcr-3.5</i> was identified on distinct plasmid backbones: an IncFIB-type plasmid in AMR-0220 and an IncFII-type plasmid in AMR-0361. Overall, our findings demonstrate that the <i>mcr</i> genes found in <i>E. coli</i> isolates in this region are located on different mobile genetic elements, indicating the potential for a widespread dissemination of colistin resistance among Gram-negative bacteria throughout Thailand's healthcare system.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 6","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189599/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Co-Existence of <i>mcr-1.1</i> and <i>mcr-3.5</i> in <i>Escherichia coli</i> Isolated from Clinical Samples in Thailand.\",\"authors\":\"Panida Nobthai, Sirigade Ruekit, Dutsadee Peerapongpaisarn, Prawet Sukhchat, Brett E Swierczewski, Nattaya Ruamsap, Paphavee Lertsethtakarn\",\"doi\":\"10.3390/antibiotics14060596\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The emergence of colistin resistance poses a significant threat to its efficacy as a last-line treatment against multidrug-resistant Gram-negative bacterial infections. In this study, 178 multi-drug resistant (MDR) <i>Escherichia coli</i> isolates collected from clinical samples at Queen Sirikit Naval Hospital, Chonburi, Thailand, were evaluated for colistin resistance. Of these, six were identified as <i>mcr</i> gene carriers, mediating colistin resistance. Specifically, <i>mcr-1</i> was detected in three <i>E. coli</i> isolates, <i>mcr-3</i> was detected in one <i>E. coli</i> isolate, and <i>mcr-1</i> and <i>mcr-3</i> were detected in two <i>E. coli</i> isolates, designated AMR-0220 and AMR-0361. Whole-genome sequencing and bioinformatics analysis revealed that AMR-0220 and AMR-0361 belonged to ST410 and ST617 lineages, respectively. Both isolates carried multiple plasmids, with <i>mcr-1.1</i> located on an IncX4-type plasmid that is closely related to previously reported <i>mcr-1.1</i>-carrying IncX4 plasmids. In contrast, <i>mcr-3.5</i> was identified on distinct plasmid backbones: an IncFIB-type plasmid in AMR-0220 and an IncFII-type plasmid in AMR-0361. Overall, our findings demonstrate that the <i>mcr</i> genes found in <i>E. coli</i> isolates in this region are located on different mobile genetic elements, indicating the potential for a widespread dissemination of colistin resistance among Gram-negative bacteria throughout Thailand's healthcare system.</p>\",\"PeriodicalId\":54246,\"journal\":{\"name\":\"Antibiotics-Basel\",\"volume\":\"14 6\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2025-06-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189599/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antibiotics-Basel\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3390/antibiotics14060596\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibiotics-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antibiotics14060596","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

摘要

粘菌素耐药性的出现对其作为抗多重耐药革兰氏阴性细菌感染的最后一线治疗的有效性构成了重大威胁。在本研究中,对从泰国春武里诗丽吉皇后海军医院临床样本中收集的178株多重耐药(MDR)大肠杆菌进行了粘菌素耐药性评估。其中,6个被鉴定为mcr基因携带者,介导粘菌素耐药性。其中,3株大肠杆菌中检测到mcr-1, 1株大肠杆菌中检测到mcr-3, 2株大肠杆菌中检测到mcr-1和mcr-3,分别命名为AMR-0220和AMR-0361。全基因组测序和生物信息学分析表明,AMR-0220和AMR-0361分别归属于ST410和ST617家系。两个分离株都携带多个质粒,其中mcr-1.1位于一个IncX4型质粒上,与先前报道的携带IncX4质粒的mcr-1.1密切相关。相比之下,mcr-3.5在不同的质粒主干上被鉴定出来:AMR-0220中的incfib型质粒和AMR-0361中的incfii型质粒。总体而言,我们的研究结果表明,在该地区大肠杆菌分离株中发现的mcr基因位于不同的可移动遗传元件上,这表明在泰国整个医疗保健系统中,革兰氏阴性菌中粘菌素耐药性的广泛传播潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Co-Existence of mcr-1.1 and mcr-3.5 in Escherichia coli Isolated from Clinical Samples in Thailand.

The emergence of colistin resistance poses a significant threat to its efficacy as a last-line treatment against multidrug-resistant Gram-negative bacterial infections. In this study, 178 multi-drug resistant (MDR) Escherichia coli isolates collected from clinical samples at Queen Sirikit Naval Hospital, Chonburi, Thailand, were evaluated for colistin resistance. Of these, six were identified as mcr gene carriers, mediating colistin resistance. Specifically, mcr-1 was detected in three E. coli isolates, mcr-3 was detected in one E. coli isolate, and mcr-1 and mcr-3 were detected in two E. coli isolates, designated AMR-0220 and AMR-0361. Whole-genome sequencing and bioinformatics analysis revealed that AMR-0220 and AMR-0361 belonged to ST410 and ST617 lineages, respectively. Both isolates carried multiple plasmids, with mcr-1.1 located on an IncX4-type plasmid that is closely related to previously reported mcr-1.1-carrying IncX4 plasmids. In contrast, mcr-3.5 was identified on distinct plasmid backbones: an IncFIB-type plasmid in AMR-0220 and an IncFII-type plasmid in AMR-0361. Overall, our findings demonstrate that the mcr genes found in E. coli isolates in this region are located on different mobile genetic elements, indicating the potential for a widespread dissemination of colistin resistance among Gram-negative bacteria throughout Thailand's healthcare system.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Antibiotics-Basel
Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍: Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信