是否有可能优化万古霉素导管锁定溶液的制备和保存?

IF 4.3 2区 医学 Q1 INFECTIOUS DISEASES
Marta Díaz-Navarro, David Samitier, Félix García-Moreno, María Sanjurjo, Patricia Muñoz, Beatriz Torroba, María Guembe
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引用次数: 0

摘要

背景/目的:万古霉素被广泛用于导管锁定治疗。然而,它的特设准备在药房部门涉及丢弃大部分静脉小瓶,并有助于高工作量。我们的目的是评估冷冻V锁溶液的V浓度和最低抑制生物膜浓度(MIBC)。方法:采用V +肝素100 IU/mL和V +柠檬酸2%两种V-2 mg/mL溶液进行试验。溶液在-20°C冷冻,随后冷藏48小时,并在基线和2、4、8和12周后进行分析(实验1)。另外,12周冷冻期结束后,溶液1在4℃和室温下分别保存1周和2周(实验2)。采用HPLC-DAD法测定205 nm下的V浓度,并进行强制降解试验。金黄色葡萄球菌(MSSA, MRSA),表皮葡萄球菌ATCC35984 (SE),以及高度生物膜形成的临床表皮葡萄球菌菌株(SEclin)。MIBC定义为代谢活性降低≥50%。结果:在实验1中,虽然V浓度随时间保持稳定,但MIBC值发生变化,从8周开始,所有菌株的MIBC值都显著增加。此外,在实验2中,在2周的时间内检测到V浓度和MIBC的显著降低。结论:V锁溶液似乎可以冷冻12周,然后在冷藏或室温下保存1周。这有利于医院药房实验室小瓶制备的优化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Is It Possible to Optimize the Elaboration and Preservation of a Vancomycin Catheter Lock Solution?

Background/Objectives: Vancomycin (V) is widely used for catheter lock therapy. However, its ad hoc preparation in pharmacy departments involves discarding most of an intravenous vial and contributes to high workload. We aimed to assess the V concentration and minimum inhibitory biofilm concentration (MIBC) of a frozen V lock solution. Methods: Two V-2 mg/mL solutions were tested: (1) V + heparin 100 IU/mL and (2) V + citrate 2%. Solutions were frozen at -20 °C, followed by 48 h refrigeration, and analyses were performed at baseline and after 2, 4, 8, and 12 weeks (experiment 1). In addition, after the 12-week freezing period, solution 1 was also preserved for 1 and 2 weeks at both 4 °C and room temperature (experiment 2). V concentration was assessed by HPLC-DAD at 205 nm and validated with forced degradation tests. A <10% variation indicated significant change. MBIC was determined by XTT staining of 24 h biofilms exposed to decreasing concentrations of each solution. Microorganisms tested included methicillin-susceptible and -resistant Staphylococcus aureus (MSSA, MRSA), Staphylococcus epidermidis ATCC35984 (SE), and a highly biofilm-forming clinical S. epidermidis strain (SEclin). MIBC was defined as ≥50% reduction in metabolic activity. Results: In experiment 1, while V concentration remained stable over time, MIBC values varied, notably increasing from 8 weeks for all strains. Moreover, in experiment 2, significant reductions in both V concentration and MIBC were detected in the 2-week period. Conclusions: V lock solution appears to be able to be 12-weeks frozen followed by up to 1 week at refrigeration or room temperature. This facilitates the optimization of vial preparation in hospital pharmacy laboratories.

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来源期刊
Antibiotics-Basel
Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍: Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.
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