Cihan Yüksel, Işıl D Alıravcı, Murat Koşan, Sinem Esen, Sevinç Yenice Aktaş, Neslihan Kaya Terzi, Ahmet Ali Berber, Sevil Alkan, Selçuk Kaya
{"title":"膀胱内给药替加环素治疗广泛耐药（XDR）、替加环素敏感鲍曼不动杆菌引起的膀胱炎的疗效研究。","authors":"Cihan Yüksel, Işıl D Alıravcı, Murat Koşan, Sinem Esen, Sevinç Yenice Aktaş, Neslihan Kaya Terzi, Ahmet Ali Berber, Sevil Alkan, Selçuk Kaya","doi":"10.3390/antibiotics14060611","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the therapeutic efficacy of intravesical tigecycline administration in a rat model of cystitis induced by a tigecycline-sensitive, extensively drug-resistant (XDR) <i>Acinetobacter baumannii</i> strain.</p><p><strong>Methods: </strong>Thirty-six female Wistar albino rats were inoculated intravesically with XDR <i>A. baumannii</i> to induce cystitis. Twenty-four rats that developed infection were divided into four groups: untreated control, saline irrigation, low-dose tigecycline (6.25 mg/kg), and high-dose tigecycline (25 mg/kg). Microbiological clearance was assessed via urine cultures on days 3 and 5. Bladder tissues were analyzed histopathologically and for genotoxicity using the Comet assay.</p><p><strong>Results: </strong>On day 5, microbiological clearance was significantly higher in tigecycline-treated groups compared to controls (<i>p</i> = 0.028). Histopathology revealed significantly more inflammation in the high-dose tigecycline group (<i>p</i> = 0.029). Genotoxicity was observed in both tigecycline groups, independent of dose (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>Intravesical tigecycline demonstrated microbiological efficacy against XDR <i>A. baumannii</i>-induced cystitis. However, its inflammatory and genotoxic potential necessitates further preclinical evaluation.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 6","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189304/pdf/","citationCount":"0","resultStr":"{\"title\":\"Investigation of Therapeutic Efficacy of Intravesical Tigecycline Administration in Rats with Cystitis Induced by Extensively Drug-Resistant (XDR), Tigecycline-Sensitive <i>Acinetobacter baumannii</i> Strain.\",\"authors\":\"Cihan Yüksel, Işıl D Alıravcı, Murat Koşan, Sinem Esen, Sevinç Yenice Aktaş, Neslihan Kaya Terzi, Ahmet Ali Berber, Sevil Alkan, Selçuk Kaya\",\"doi\":\"10.3390/antibiotics14060611\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>This study aimed to evaluate the therapeutic efficacy of intravesical tigecycline administration in a rat model of cystitis induced by a tigecycline-sensitive, extensively drug-resistant (XDR) <i>Acinetobacter baumannii</i> strain.</p><p><strong>Methods: </strong>Thirty-six female Wistar albino rats were inoculated intravesically with XDR <i>A. baumannii</i> to induce cystitis. Twenty-four rats that developed infection were divided into four groups: untreated control, saline irrigation, low-dose tigecycline (6.25 mg/kg), and high-dose tigecycline (25 mg/kg). Microbiological clearance was assessed via urine cultures on days 3 and 5. Bladder tissues were analyzed histopathologically and for genotoxicity using the Comet assay.</p><p><strong>Results: </strong>On day 5, microbiological clearance was significantly higher in tigecycline-treated groups compared to controls (<i>p</i> = 0.028). Histopathology revealed significantly more inflammation in the high-dose tigecycline group (<i>p</i> = 0.029). Genotoxicity was observed in both tigecycline groups, independent of dose (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>Intravesical tigecycline demonstrated microbiological efficacy against XDR <i>A. baumannii</i>-induced cystitis. However, its inflammatory and genotoxic potential necessitates further preclinical evaluation.</p>\",\"PeriodicalId\":54246,\"journal\":{\"name\":\"Antibiotics-Basel\",\"volume\":\"14 6\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2025-06-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189304/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antibiotics-Basel\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3390/antibiotics14060611\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibiotics-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antibiotics14060611","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Investigation of Therapeutic Efficacy of Intravesical Tigecycline Administration in Rats with Cystitis Induced by Extensively Drug-Resistant (XDR), Tigecycline-Sensitive Acinetobacter baumannii Strain.
Background: This study aimed to evaluate the therapeutic efficacy of intravesical tigecycline administration in a rat model of cystitis induced by a tigecycline-sensitive, extensively drug-resistant (XDR) Acinetobacter baumannii strain.
Methods: Thirty-six female Wistar albino rats were inoculated intravesically with XDR A. baumannii to induce cystitis. Twenty-four rats that developed infection were divided into four groups: untreated control, saline irrigation, low-dose tigecycline (6.25 mg/kg), and high-dose tigecycline (25 mg/kg). Microbiological clearance was assessed via urine cultures on days 3 and 5. Bladder tissues were analyzed histopathologically and for genotoxicity using the Comet assay.
Results: On day 5, microbiological clearance was significantly higher in tigecycline-treated groups compared to controls (p = 0.028). Histopathology revealed significantly more inflammation in the high-dose tigecycline group (p = 0.029). Genotoxicity was observed in both tigecycline groups, independent of dose (p < 0.05).
Conclusions: Intravesical tigecycline demonstrated microbiological efficacy against XDR A. baumannii-induced cystitis. However, its inflammatory and genotoxic potential necessitates further preclinical evaluation.
Antibiotics-BaselPharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍：
Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.
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