5-氟吲哚抑制拟靶蛋氨酸合成酶和丁香假单胞菌。actinidiae毒性

IF 6.2 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Chun Zhang, Kunhong Zhao, Zilin Wu, Na Tao, Weijia Yang, Zhaojia Li, Moxian Chen* and Xiangyang Li*, 
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引用次数: 0

摘要

由丁香假单胞菌引起的细菌性溃疡病。actinidiae (Psa)严重影响全球猕猴桃生产。本研究表明,5-氟吲哚对Psa具有显著的杀菌活性,其半最大有效浓度(EC50)为15.34 μg/mL,高于阳性对照氢氧化铜(EC50 = 58.65 μg/mL)。5-氟吲哚破坏细胞膜完整性,诱导活性氧(ROS)积累,并引发Psa细胞凋亡。转录组分析显示,5-氟吲哚处理使蛋氨酸合成酶II (MetE)基因的表达比对照(CK)组增加了6.28倍。微尺度热电泳和等温滴定量热法表明,5-氟吲哚与MetE蛋白结合的解离常数分别为0.33 μM和8.55 μM。分子对接实验表明,Asp693是关键结合位点,5-氟吲哚与Psa MetED693A突变体之间没有特异性结合。本研究为开发有效防治猕猴桃细菌性溃疡病的药剂提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

5-Fluoroindole Inhibits Its Putative Target Methionine Synthase and Pseudomonas syringae pv. actinidiae Virulence

5-Fluoroindole Inhibits Its Putative Target Methionine Synthase and Pseudomonas syringae pv. actinidiae Virulence

5-Fluoroindole Inhibits Its Putative Target Methionine Synthase and Pseudomonas syringae pv. actinidiae Virulence

Bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) severely impacts global kiwifruit production. This study shows that 5-fluoroindole exerts significant bactericidal activity against Psa, with a half-maximal effective concentration (EC50) of 15.34 μg/mL, demonstrating a higher efficacy than the positive control copper hydroxide (EC50 = 58.65 μg/mL). 5-Fluoroindole disrupts membrane integrity, induces oxygen species (ROS) accumulation, and triggers apoptosis in Psa. Transcriptome analysis reveals that 5-fluoroindole treatment increased the expression of the methionine synthase II (MetE) gene by 6.28 fold compared with the expression of this gene in the control (CK) group. Microscale thermophoresis and isothermal titration calorimetry show that the dissociation constants of the binding of 5-fluoroindole to MetE protein are 0.33 and 8.55 μM, respectively. Molecular docking experiments indicate that Asp693 is a key binding site and that there is no specific binding between 5-fluoroindole and the Psa MetED693A mutant. This study provides valuable insights for developing effective agents to control kiwifruit bacterial canker.

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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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