微生物无细胞DNA检测在儿科头颈部感染患者中检测病原体的有效性初步研究。

IF 1.9 4区医学 Q2 PEDIATRICS

Pediatric Investigation Pub Date : 2025-01-06 eCollection Date: 2025-06-01 DOI:10.1002/ped4.12462

Audrey Xu, Andrea Miranda, Hung Fu Lin, Taseer Din, Karthik Balakrishnan, Mai Thy Truong, Iram Ahmad, Kara Meister

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引用次数: 0

摘要

我们研究了血液中微生物无细胞DNA （mcfDNA）的效用，以预测手术培养结果。在这项研究中，只有26.9%的患者的mcfDNA结果被认为可以预测致病生物。在队列中存在显著的污染负担，强调在进行基于基因的诊断时必须遵循专门的协议。健康患者显示出可检测到的mcfDNA信号，尽管对于类似的病原体来说，每毫升的mcfDNA分子通常较低。

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Efficacy of microbial cell-free DNA testing for detecting pathogens in pediatric patients with head and neck infections-An initial study.

We investigate the utility of microbial cell-free DNA (mcfDNA) from the blood to predict surgical culture results. In this study, only 26.9% of patients had mcfDNA results that were considered predictive of the causative organism(s). There was a significant burden of contamination in the cohort, emphasizing that dedicated protocols must be followed when running gene-based diagnostics. Healthy patients showed detectable mcfDNA signals, though typically at lower molecules per milliliter for similar pathogens.

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