基于Fe3O4@Au@PAMAM和AuNs@4-MBA@Au的新型竞争SERS/电化学双通道生物传感器用于Pb2+的超灵敏定量检测

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2025-06-19 DOI:10.1016/j.jfca.2025.107933

Xinran Yang , Zonglin Li , Junhui Du , Xueli Zhang , Haibin Liu , Xuechao Zhang , Lijun Li , Chuanjin Cui , Hongshuo Chen

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引用次数: 0

摘要

本文建立了一种SERS/电化学双通道适体生物传感器，用于Pb2+的超灵敏检测。首先，采用化学方法一步一步合成捕获探针Fe3O4@Au@PAMAM@Aptamer和信号探针AuNs@4-MBA@Au@ssDNA。其次，在电极表面电沉积羧基氧化石墨烯（GO），获得GO/SPCE；同时，成功合成了PAMAM@Au纳米材料，制备了Aptamer/PAMAM@Au/GO/SPCE。在此过程中，利用SEM、TEM、FTIR和XPS对合成的纳米材料进行了表征，证实了纳米材料的成功合成。最后，采用表面增强拉曼（SERS）和差分脉冲伏安法（DPV）在一系列Pb2+水溶液中评价了该生物传感器的性能。结果表明，该方法的最低检出限为3.16 × 10−5 nM，样品回收率为99.50 ~ 103.67 %。此外，该生物传感器具有良好的特异性、可重复性和稳定性。在实际样品试验中也得到了可靠的结果。因此，为重金属离子的痕量检测提供了一种可行的方法。

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Novel competitive SERS/Electrochemical dual-channel biosensor for ultrasensitive quantitative detection of Pb2+ based on Fe3O4@Au@PAMAM and AuNs@4-MBA@Au

In this paper, a SERS/electrochemical dual-channel aptamer biosensor was established for ultrasensitive detection of Pb²⁺. Firstly, the capture probe Fe₃O₄@Au@PAMAM@Aptamer and the signal probe AuNs@4-MBA@Au@ssDNA were chemically synthesized step by step. Secondly, the carboxyl graphene oxide (GO) was electrodeposited onto the electrode surface to gain the GO/SPCE. Meanwhile, PAMAM@Au nanomaterials were successfully synthesized and thus prepared the Aptamer/PAMAM@Au/GO/SPCE. During the process, SEM, TEM, FTIR and XPS were applied to characterize the synthesized nanomaterials which confirmed the successful synthesis of the nanomaterials. Finaly, the performance of this biosensor was evaluated in a series of Pb²⁺ aqueous solutions by surface enhanced Raman (SERS) and differential pulse voltammetry (DPV). The results showed that the lowest limit of detection (LOD) was 3.16 × 10⁻⁵ nM, and the sample recovery was 99.50–103.67 %. Furthermore, the biosensor demonstrated good specificity, repeatability, and stability. Reliable results were also obtained in actual sample testing. Therefore, it can provide a viable method for trace detection of heavy metal ions.

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.