秀丽隐杆线虫实时表型筛选的线扫描成像技术。

IF 1.3 4区工程技术 Q3 INSTRUMENTS & INSTRUMENTATION

Review of Scientific Instruments Pub Date : 2025-06-01 DOI:10.1063/5.0238824

Aaron Au, Maximiliano Giuliani, Sean Harrington, Peter Roy, Christopher M Yip

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引用次数: 0

摘要

生物体的表型筛选通常需要实时、高空间分辨率的成像。我们在这里报告了一种线扫描成像平台的设计和应用，该平台可以使用96孔板配置对活线虫进行亮场成像。系统光学是这样的，每个单独的井被投射在一个4 K线扫描相机的整个视野。为了最大限度地减少运动伪影，相机系统被平移，而样品保持静止。我们讨论了在建立平场照明、图像配准和拼接方面的挑战，以及该平台为生命系统的表型筛选提供的机会。

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Line-scan imaging for real-time phenotypic screening of C. elegans.

Phenotypic screens of living organisms often require real-time, high spatial resolution imaging. We report here on the design and application of a line-scan imaging platform that enables bright-field imaging of live nematodes using a 96-well plate configuration. The system optics are such that each individual well is projected across the full field of view of a 4 K line scanning camera. To minimize motional artifacts, the camera system is translated, while the sample is kept stationary. We discuss the challenges in establishing flat field illumination, image registration and stitching, and the opportunities that this platform has enabled for phenotypic screens of living systems.

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来源期刊

Review of Scientific Instruments 工程技术-物理：应用

CiteScore

3.00

自引率

12.50%

发文量

758

审稿时长

2.6 months

期刊介绍： Review of Scientific Instruments, is committed to the publication of advances in scientific instruments, apparatuses, and techniques. RSI seeks to meet the needs of engineers and scientists in physics, chemistry, and the life sciences.