Amanda L Miller, John A Renye, David B Johnston, Jennifer Thomas, Adam M Oest, Rafael Garcia, Peggy M Tomasula
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引用次数: 0
摘要
将食物垃圾或食物副产品转化为高价值产品,可以为食品供应链做出贡献,从而使食品工业受益。本研究旨在探讨利用废冰淇淋作为酵母生产乙醇的发酵底物的潜力。结果表明,2株酿酒酵母Lalvin EC-1118 (Lallemand Inc.)和Ethanol Red (Lesaffre)能够对废冰淇淋的水产物进行发酵,产生约7% (wt/vol)的乙醇。这很可能是由于利用了游离单糖或蔗糖,因为它们不能发酵乳糖。通过添加乳糖酶和葡萄糖淀粉酶来提高可发酵碳水化合物的利用率,Lalvin EC-118和乙醇红菌株的乙醇产量分别为9.77%和9.30% (wt/vol)。这两种菌株也被证明不利用乳糖水解后释放的半乳糖。在乳糖酶和蛋白酶联合处理的样品中,最大的乙醇产量超过13% (wt/vol),这表明缺乏氮使发酵停滞。用蛋白酶、乳糖酶和磷酸二铵处理的发酵香草冰淇淋进行了台式蒸馏。蒸馏是成功的,并收集了几种不同的香气和气味的馏分。这项研究的结果表明,乙醇生产是一种可行的增值策略,可以从废弃的冰淇淋中生产蒸馏饮料酒精,预计将不含蛋白质过敏原。
Waste ice cream is the yeast of our problems: The valorization of waste ice cream into alcohol.
Valorization of food waste or food by-products into higher-value products can benefit the food industry by contributing to the food supply chain. This study aimed to investigate the potential for using waste ice cream as a fermentation substrate for production of ethanol by yeast. Results showed that 2 Saccharomyces cerevisiae strains, Lalvin EC-1118 (Lallemand Inc.) and Ethanol Red (Lesaffre), were able to ferment an aqueous by-product of waste ice cream, resulting in the production of ethanol, ~7% (wt/vol). This most likely occurred due to the utilization of free monosaccharides or sucrose, because they were unable to ferment lactose. Lactase and glucoamylase were added, to increase the availability of fermentable carbohydrates, resulting in 9.77% and 9.30% (wt/vol) ethanol produced by Lalvin EC-118 and Ethanol Red strains, respectively. Both strains were also shown to not utilize the galactose released following the hydrolysis of lactose. Maximum ethanol production, above 13% (wt/vol), was seen in samples treated with a combination of lactase and a protease, suggesting that a lack of nitrogen was stalling the fermentation. A benchtop-scale distillation was performed using a fermented vanilla ice cream treated with protease, lactase, and diammonium phosphate. The distillation was successful, and several fractions with various aromas and odors were collected. Results from this study suggest that that ethanol production is a viable valorization strategy to produce distilled beverage alcohol from waste ice cream, which is expected to be free of protein allergens.
期刊介绍:
The official journal of the American Dairy Science Association®, Journal of Dairy Science® (JDS) is the leading peer-reviewed general dairy research journal in the world. JDS readers represent education, industry, and government agencies in more than 70 countries with interests in biochemistry, breeding, economics, engineering, environment, food science, genetics, microbiology, nutrition, pathology, physiology, processing, public health, quality assurance, and sanitation.