水杨PLL基因家族的鉴定及AoPLL17在盐胁迫下的功能分析

IF 3.9 2区 农林科学 Q1 HORTICULTURE
Haihang Yu , Yanmei Ma , Xuanyu Shen , Tianshu Wang , Na Li , Yunwei Zhou , Yun Bai
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引用次数: 0

摘要

果胶裂解酶样(PLL)通过解聚均半乳糖酸在植物生长中起核心作用,影响植物细胞壁的结构和组成。然而,目前还没有对水杨花(Aquilegia oxysepala)的PLL家族进行系统的分析,并且观赏植物PLL与盐胁迫的关系还有待阐明。在本研究中,我们从A. oxysepala中鉴定了17个PLL基因,并通过系统发育分析将AoPLL分为5个类群。大多数AoPLL基因在盐胁迫下表现出表达水平的改变,其中AoPLL17基因表现出显著的表达变化。对apll17基因的组织特异性表达模式分析表明,该基因在叶柄中表达水平最高。apll17蛋白定位于细胞质膜系统。功能研究表明,AoPLL17的过表达增加了转基因烟草对盐胁迫的敏感性。与野生型植物相比,转基因品系在盐胁迫下表现出更明显的植物生长限制、活性氧清除能力下降和光合能力下降。此外,转基因AoPLL17烟草植株的细胞形态发生改变,细胞壁变薄,果胶含量降低。综上所述,AoPLL17通过影响活性氧清除水平、光合能力和维持细胞壁完整性,在盐胁迫响应中发挥了重要作用。本研究为探索花青树PLL的功能提供了数据参考,也为提高观赏植物的抗性提供了新的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of the PLL gene family in Aquilegia oxysepala and functional analysis of AoPLL17 under salt stress
Pectate lyase-like (PLL) plays a central role in plant growth by depolymerizing homogalacturonan, which can affect the structure and composition of plant cell walls. However, the PLL family in Aquilegia oxysepala has not yet been systematically analysed, and the association of PLL with salt stress in ornamentals remains to be elucidated. In the present study, we have identified 17 PLL genes from the A. oxysepala and have classified AoPLL into five groups through phylogenetic analysis. The majority of AoPLL genes exhibited altered expression levels in response to salt stress, with the AoPLL17 gene demonstrating significant changes in expression. The analysis of tissue-specific expression patterns of the AoPLL17 gene revealed that it was expressed at the highest level in leafstalks. The protein AoPLL17 is localised to the cytoplasmic membrane system. Functional studies demonstrated that the overexpression of AoPLL17 increased the sensitivity of transgenic tobacco plants to salt stress. The transgenic lines exhibited more pronounced restrictions in plant growth, reduced scavenging of ROS, and diminished photosynthetic capacity due to salt stress than the wild type plants. Additionally, transgenic AoPLL17 tobacco plants displayed altered cell morphology, thinner cell walls, and decreased pectin content. In conclusion, AoPLL17 performs a significant function in the response to salt stress by influencing the level of ROS scavenging, photosynthetic capacity, and maintenance of cell wall integrity. This study provides a data reference for exploring the function of the PLL in A. oxysepala and a new target for the improvement of ornamental plant resistance.
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来源期刊
Scientia Horticulturae
Scientia Horticulturae 农林科学-园艺
CiteScore
8.60
自引率
4.70%
发文量
796
审稿时长
47 days
期刊介绍: Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.
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