Haihang Yu , Yanmei Ma , Xuanyu Shen , Tianshu Wang , Na Li , Yunwei Zhou , Yun Bai
{"title":"水杨PLL基因家族的鉴定及AoPLL17在盐胁迫下的功能分析","authors":"Haihang Yu , Yanmei Ma , Xuanyu Shen , Tianshu Wang , Na Li , Yunwei Zhou , Yun Bai","doi":"10.1016/j.scienta.2025.114214","DOIUrl":null,"url":null,"abstract":"<div><div><em>Pectate lyase-like</em> (<em>PLL</em>) plays a central role in plant growth by depolymerizing homogalacturonan, which can affect the structure and composition of plant cell walls. However, the <em>PLL</em> family in <em>Aquilegia oxysepala</em> has not yet been systematically analysed, and the association of <em>PLL</em> with salt stress in ornamentals remains to be elucidated. In the present study, we have identified 17 <em>PLL</em> genes from the <em>A. oxysepala</em> and have classified <em>AoPLL</em> into five groups through phylogenetic analysis. The majority of <em>AoPLL</em> genes exhibited altered expression levels in response to salt stress, with the <em>AoPLL17</em> gene demonstrating significant changes in expression. The analysis of tissue-specific expression patterns of the <em>AoPLL17</em> gene revealed that it was expressed at the highest level in leafstalks. The protein AoPLL17 is localised to the cytoplasmic membrane system. Functional studies demonstrated that the overexpression of <em>AoPLL17</em> increased the sensitivity of transgenic tobacco plants to salt stress. The transgenic lines exhibited more pronounced restrictions in plant growth, reduced scavenging of ROS, and diminished photosynthetic capacity due to salt stress than the wild type plants. Additionally, transgenic <em>AoPLL17</em> tobacco plants displayed altered cell morphology, thinner cell walls, and decreased pectin content. In conclusion, <em>AoPLL17</em> performs a significant function in the response to salt stress by influencing the level of ROS scavenging, photosynthetic capacity, and maintenance of cell wall integrity. This study provides a data reference for exploring the function of the <em>PLL</em> in <em>A. oxysepala</em> and a new target for the improvement of ornamental plant resistance.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"348 ","pages":"Article 114214"},"PeriodicalIF":3.9000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of the PLL gene family in Aquilegia oxysepala and functional analysis of AoPLL17 under salt stress\",\"authors\":\"Haihang Yu , Yanmei Ma , Xuanyu Shen , Tianshu Wang , Na Li , Yunwei Zhou , Yun Bai\",\"doi\":\"10.1016/j.scienta.2025.114214\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Pectate lyase-like</em> (<em>PLL</em>) plays a central role in plant growth by depolymerizing homogalacturonan, which can affect the structure and composition of plant cell walls. However, the <em>PLL</em> family in <em>Aquilegia oxysepala</em> has not yet been systematically analysed, and the association of <em>PLL</em> with salt stress in ornamentals remains to be elucidated. In the present study, we have identified 17 <em>PLL</em> genes from the <em>A. oxysepala</em> and have classified <em>AoPLL</em> into five groups through phylogenetic analysis. The majority of <em>AoPLL</em> genes exhibited altered expression levels in response to salt stress, with the <em>AoPLL17</em> gene demonstrating significant changes in expression. The analysis of tissue-specific expression patterns of the <em>AoPLL17</em> gene revealed that it was expressed at the highest level in leafstalks. The protein AoPLL17 is localised to the cytoplasmic membrane system. Functional studies demonstrated that the overexpression of <em>AoPLL17</em> increased the sensitivity of transgenic tobacco plants to salt stress. The transgenic lines exhibited more pronounced restrictions in plant growth, reduced scavenging of ROS, and diminished photosynthetic capacity due to salt stress than the wild type plants. Additionally, transgenic <em>AoPLL17</em> tobacco plants displayed altered cell morphology, thinner cell walls, and decreased pectin content. In conclusion, <em>AoPLL17</em> performs a significant function in the response to salt stress by influencing the level of ROS scavenging, photosynthetic capacity, and maintenance of cell wall integrity. This study provides a data reference for exploring the function of the <em>PLL</em> in <em>A. oxysepala</em> and a new target for the improvement of ornamental plant resistance.</div></div>\",\"PeriodicalId\":21679,\"journal\":{\"name\":\"Scientia Horticulturae\",\"volume\":\"348 \",\"pages\":\"Article 114214\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientia Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0304423825002638\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304423825002638","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
Identification of the PLL gene family in Aquilegia oxysepala and functional analysis of AoPLL17 under salt stress
Pectate lyase-like (PLL) plays a central role in plant growth by depolymerizing homogalacturonan, which can affect the structure and composition of plant cell walls. However, the PLL family in Aquilegia oxysepala has not yet been systematically analysed, and the association of PLL with salt stress in ornamentals remains to be elucidated. In the present study, we have identified 17 PLL genes from the A. oxysepala and have classified AoPLL into five groups through phylogenetic analysis. The majority of AoPLL genes exhibited altered expression levels in response to salt stress, with the AoPLL17 gene demonstrating significant changes in expression. The analysis of tissue-specific expression patterns of the AoPLL17 gene revealed that it was expressed at the highest level in leafstalks. The protein AoPLL17 is localised to the cytoplasmic membrane system. Functional studies demonstrated that the overexpression of AoPLL17 increased the sensitivity of transgenic tobacco plants to salt stress. The transgenic lines exhibited more pronounced restrictions in plant growth, reduced scavenging of ROS, and diminished photosynthetic capacity due to salt stress than the wild type plants. Additionally, transgenic AoPLL17 tobacco plants displayed altered cell morphology, thinner cell walls, and decreased pectin content. In conclusion, AoPLL17 performs a significant function in the response to salt stress by influencing the level of ROS scavenging, photosynthetic capacity, and maintenance of cell wall integrity. This study provides a data reference for exploring the function of the PLL in A. oxysepala and a new target for the improvement of ornamental plant resistance.
期刊介绍:
Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.