Daniel H. Lysak, Carl A. Michal, Kathryn Marable, Marco Grisi, Reza Farsi, Peter M. Costa, Jacob Pellizzari, Vincent Moxley-Paquette, Katelyn Downey, Kiera Ronda, Owen Vander Meulen, William W. Wolff, Katrina Steiner, Flavio V. C. Kock, Tiago B. Moraes, Luiz A. Colnago, Felix Schuderer, Tobias Wirth, Myrna Simpson, Jens Anders and Andre J. Simpson*,
{"title":"用于环境样品高通量分析的“仅接收”核磁共振互补金属氧化物半导体微线圈阵列","authors":"Daniel H. Lysak, Carl A. Michal, Kathryn Marable, Marco Grisi, Reza Farsi, Peter M. Costa, Jacob Pellizzari, Vincent Moxley-Paquette, Katelyn Downey, Kiera Ronda, Owen Vander Meulen, William W. Wolff, Katrina Steiner, Flavio V. C. Kock, Tiago B. Moraes, Luiz A. Colnago, Felix Schuderer, Tobias Wirth, Myrna Simpson, Jens Anders and Andre J. Simpson*, ","doi":"10.1021/acs.analchem.5c0095510.1021/acs.analchem.5c00955","DOIUrl":null,"url":null,"abstract":"<p ><i>Daphnia magna</i> neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, mass-limited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three <i>D. magna</i> eggs concurrently, potentially opening the door for high-throughput analysis and toxicological studies, where the control and exposed cohorts can be studied concurrently, reducing day-to-day variability. Overall, microcoil arrays show considerable promise for the study of <i>D. magna</i> eggs and other mass-limited samples.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 22","pages":"11643–11652 11643–11652"},"PeriodicalIF":6.7000,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Toward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples\",\"authors\":\"Daniel H. Lysak, Carl A. Michal, Kathryn Marable, Marco Grisi, Reza Farsi, Peter M. Costa, Jacob Pellizzari, Vincent Moxley-Paquette, Katelyn Downey, Kiera Ronda, Owen Vander Meulen, William W. Wolff, Katrina Steiner, Flavio V. C. Kock, Tiago B. Moraes, Luiz A. Colnago, Felix Schuderer, Tobias Wirth, Myrna Simpson, Jens Anders and Andre J. Simpson*, \",\"doi\":\"10.1021/acs.analchem.5c0095510.1021/acs.analchem.5c00955\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p ><i>Daphnia magna</i> neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, mass-limited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three <i>D. magna</i> eggs concurrently, potentially opening the door for high-throughput analysis and toxicological studies, where the control and exposed cohorts can be studied concurrently, reducing day-to-day variability. Overall, microcoil arrays show considerable promise for the study of <i>D. magna</i> eggs and other mass-limited samples.</p>\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"97 22\",\"pages\":\"11643–11652 11643–11652\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2025-05-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.analchem.5c00955\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.analchem.5c00955","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Toward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples
Daphnia magna neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, mass-limited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three D. magna eggs concurrently, potentially opening the door for high-throughput analysis and toxicological studies, where the control and exposed cohorts can be studied concurrently, reducing day-to-day variability. Overall, microcoil arrays show considerable promise for the study of D. magna eggs and other mass-limited samples.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.