用于环境样品高通量分析的“仅接收”核磁共振互补金属氧化物半导体微线圈阵列

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Daniel H. Lysak, Carl A. Michal, Kathryn Marable, Marco Grisi, Reza Farsi, Peter M. Costa, Jacob Pellizzari, Vincent Moxley-Paquette, Katelyn Downey, Kiera Ronda, Owen Vander Meulen, William W. Wolff, Katrina Steiner, Flavio V. C. Kock, Tiago B. Moraes, Luiz A. Colnago, Felix Schuderer, Tobias Wirth, Myrna Simpson, Jens Anders and Andre J. Simpson*, 
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引用次数: 0

摘要

与成年水蚤相比,大水蚤的新生儿和卵是对毒素和压力源表现出不同敏感性的关键生命阶段。核磁共振(NMR)光谱学在揭示潜在的生化原因方面具有独特的潜力,但这种非常小、质量有限的样品对研究具有挑战性。虽然微线圈的质量灵敏度的提高导致了核磁共振分析的显著改善,但实验往往仍然很长,导致低通量。微线圈阵列可以通过允许同时分析多个样品来提高吞吐量,但实际和经济上的挑战,包括昂贵的接收器和缺乏物理空间,是令人望而却步的。本文探讨了克服这些挑战的方法。首先,探索了“仅接收”平面互补金属氧化物半导体(CMOS)微线圈,其中微线圈用于检测,但外部体积线圈用于激励。与平面CMOS线圈上的激励相比,这大大改善了章动,并提供了70%的信噪比(SNR)提升。稳态自由进动实验进一步将信噪比提高了至少2.5倍,减少了采集时间,提高了吞吐量。为了完成一个三圈“仅接收”阵列,一个便宜的(315美元)软件定义的无线电板被改编成一个核磁共振接收器,其性能与商业接收器相似。三线圈阵列被用于同时研究三个D. magna卵,可能为高通量分析和毒理学研究打开大门,其中对照和暴露队列可以同时研究,减少日常变化。总的来说,微线圈阵列显示出相当大的希望,为研究D. magna卵和其他质量有限的样品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Toward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples

Toward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples

Daphnia magna neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, mass-limited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three D. magna eggs concurrently, potentially opening the door for high-throughput analysis and toxicological studies, where the control and exposed cohorts can be studied concurrently, reducing day-to-day variability. Overall, microcoil arrays show considerable promise for the study of D. magna eggs and other mass-limited samples.

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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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