用RSM优化的两水相体系纯化热带假丝酵母生成的木糖还原酶

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-06-07 DOI:10.1016/j.foodchem.2025.145069

Leanderson Tulio Marques Lemos , Lorena Lucena de Medeiros , Normando Ribeiro-Filho , Carlos Alberto Bispo de Sousa , Sharline Florentino de Melo Santos , Flávio Luiz Honorato da Silva

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引用次数: 0

摘要

木糖醇是一种甜味剂，可以由念珠菌酵母生产。由于木糖还原酶（XR）等酶的生产成本高，木糖醇生产（XP）成本高。利用腰果渣（CAW）水解可低成本生产XR；然而，XR纯化方法是昂贵的。本研究旨在利用响应面法（RSM）对木糖还原酶（XR）的ATPS进行优化。水解CAW。利用酵母（CCT 1516）发酵生产XR。然后，采用不同ATPS组成的RSM(聚乙二醇（PEG, 1.6-2.4 g/L）和磷酸钾（PP, 1-1.3 g/L）进行XR纯化。木糖醇的最高浓度在40 h时达到15.4 g/L。28 h时XR活性最高，为0.4 U/mg。RSM显示PEG对XR纯化有正向影响，而PP对XR纯化没有影响。

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Xylose reductase generated by yeast Candida tropicalis purified using an aqueous two-phase system optimised by RSM

Xylitol is a sweetener, which can be produced by Candida yeasts. Xylitol production (XP) is expensive due to the high cost of enzymes production such as xylose reductase (XR). XR can be produced with a low-cost using cashew apple waste (CAW) hydrolysed; however, methods for XR purification are expensive. This study aims to optimise an ATPS using a response surface method (RSM) for xylose reductase (XR) purification. CAW was hydrolysed. Fermentations were conducted using yeast (CCT 1516) for XR production. Then, a RSM varying ATPS composition (polyetilenglycol (PEG, 1.6–2.4 g/L) and potassium phosphate (PP, 1–1.3 g/L) was applied for XR purification. The highest concentration of xylitol (15.4 g/L) generated was achieved at 40 h. The highest XR activity (0.4 U/mg) was reached at 28 h. RSM reveals that XR purification is positively affected by PEG, in contrast, PP did not affect XR purification.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.