利用电化学活性非天然寡核苷酸与柔性微流控芯片的DNA数据存储系统

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-06-05 DOI:10.1021/acs.analchem.5c00950

Jiankai Li, Ziyan Wang, Leni Zhong, Xingyu Jiang

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引用次数: 0

摘要

引入非天然寡核苷酸可以为DNA提供更高存储密度的数据存储介质和新的数据存储范式。特别是，电化学活性的非天然寡核苷酸可以通过电化学信号检测到，允许数据存储检索数据。在这里，我们提出了一个基于DNA的电化学读出数据存储系统，该系统能够识别DNA的不同非天然电活性碱基（亚甲基蓝和二茂铁修饰碱基）。该系统采用柔性电化学微流控芯片，通过DNA杂交实现数据写入，并行电化学信号采集实现数据读取。以亚甲基蓝和二茂铁修饰的寡核苷酸为例，在电极上允许4（22）种组合进行数据存储，我们在柔性电化学微流控芯片上成功地编码和检索了一个基于四元编码的120位文本文件。该系统为DNA数据存储系统的电化学读出提供了潜在的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A DNA Data Storage System Using Electrochemically Active Non-natural Oligonucleotides with Flexible Microfluidic Chips

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A DNA Data Storage System Using Electrochemically Active Non-natural Oligonucleotides with Flexible Microfluidic Chips

Introducing non-natural oligonucleotides can provide DNA as a data storage medium with higher storage density and novel data storage paradigms. In particular, electrochemically active non-natural oligonucleotides can be detected through electrochemical signals, allowing data storage to retrieve data. Here, we present a DNA-based electrochemical readout data storage system capable of identifying different non-natural, electroactive bases (methylene blue- and ferrocene-modified bases) for DNA. The system utilizes a flexible electrochemical microfluidic chip, where data writing is achieved through DNA hybridization, and the parallel electrochemical signal acquisition enables data reading. Using methylene blue- and ferrocene-modified oligonucleotides as a demonstration, which allows 4 (2²) combinations on an electrode for the data storage, we successfully encoded and retrieved a 120-bit text file based on quaternary coding on a flexible electrochemical microfluidic chip. Our system may offer potential applications for electrochemical readout from DNA data storage system.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.