G K Silva-Cardoso, P E Bello-Junior, W Lazarini-Lopes, A A Ferrarese-Tiballi, C R A Leite-Panissi
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Anxiety-like behaviors were assessed in the open field test (OFT) 23 days after surgery. Immunofluorescence for astrocyte activity (glial fibrillary acidic protein (GFAP)) was performed in cortical, thalamic, and brainstem areas involved with pain and emotional processing. Animals submitted to NSI showed increased mechanical allodynia and thermal hyperalgesia, similar to those submitted to CCI. In the OFT, both NSI and CCI animals showed an increase in anxiety-like behaviors. Also, NSI animals presented an increased expression of GFAP in all analyzed areas, similar to CCI animals. In conclusion, the NSI model produced behavioral alterations comparable to those observed in the CCI model, including hypersensitivity to mechanical and thermal (heat) stimuli that lasted for more than three weeks. 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引用次数: 0
摘要
慢性疼痛在普通人群中的发病率与焦虑症高度相关,焦虑症对生活质量产生负面影响。拉伸损伤是平民神经功能障碍和损伤的主要原因。在这里,我们描述了低强度坐骨神经拉伸损伤(NSI)模型中伤害感受、焦虑样行为和星形胶质细胞表达的变化。雄性Wistar大鼠分别接受NSI、坐骨神经慢性收缩损伤(CCI)和假手术(sham)。术前、术后6、12、18、24天分别进行伤害性测试(von Frey、丙酮和热板)。术后23天进行开放性测试(open field test, OFT)评估焦虑样行为。免疫荧光检测与疼痛和情绪处理有关的皮质、丘脑和脑干区域的星形胶质细胞活性(胶质纤维酸性蛋白(GFAP))。NSI组的动物表现出机械异常性疼痛和热痛觉过敏的增加,与CCI组相似。在OFT中,NSI和CCI动物都表现出焦虑样行为的增加。此外,NSI动物在所有分析区域的GFAP表达均增加,与CCI动物相似。总之,NSI模型产生的行为改变与CCI模型中观察到的行为改变相当,包括对持续超过三周的机械和热刺激的超敏反应。此外,两种模型均诱导皮层、丘脑和脑干区域GFAP表达相似的增加。
Low-intensity sciatic nerve-stretch injury increases nociception, anxiety-like behaviors, and astrocyte activity in male Wistar rats.
The incidence of chronic pain in the general population is highly correlated to anxiety disorders, which promote negative effects on the quality of life. Stretch injury is the primary cause of nerve dysfunction and injury in the civilian population. Here, we characterized changes in nociception, anxiogenic-like behaviors, and astrocyte expression in the low-intensity sciatic nerve-stretch injury (NSI) model. Male Wistar rats were submitted to NSI, chronic constriction injury of the sciatic nerve (CCI), or sham surgery (SHAM). Animals were submitted to nociceptive tests (von Frey, acetone, and hot plate) before surgery and 6, 12, 18, and 24 days post-surgery. Anxiety-like behaviors were assessed in the open field test (OFT) 23 days after surgery. Immunofluorescence for astrocyte activity (glial fibrillary acidic protein (GFAP)) was performed in cortical, thalamic, and brainstem areas involved with pain and emotional processing. Animals submitted to NSI showed increased mechanical allodynia and thermal hyperalgesia, similar to those submitted to CCI. In the OFT, both NSI and CCI animals showed an increase in anxiety-like behaviors. Also, NSI animals presented an increased expression of GFAP in all analyzed areas, similar to CCI animals. In conclusion, the NSI model produced behavioral alterations comparable to those observed in the CCI model, including hypersensitivity to mechanical and thermal (heat) stimuli that lasted for more than three weeks. Additionally, both models induced a similar increase in GFAP expression in cortical, thalamic, and brainstem regions.
期刊介绍:
The Brazilian Journal of Medical and Biological Research, founded by Michel Jamra, is edited and published monthly by the Associação Brasileira de Divulgação Científica (ABDC), a federation of Brazilian scientific societies:
- Sociedade Brasileira de Biofísica (SBBf)
- Sociedade Brasileira de Farmacologia e Terapêutica Experimental (SBFTE)
- Sociedade Brasileira de Fisiologia (SBFis)
- Sociedade Brasileira de Imunologia (SBI)
- Sociedade Brasileira de Investigação Clínica (SBIC)
- Sociedade Brasileira de Neurociências e Comportamento (SBNeC).