{"title":"农杆菌介导的甜菜素对天长海棠花和叶颜色的修饰","authors":"Ikbal Karatas , Masaru Nakano , Masahiro Otani","doi":"10.1016/j.scienta.2025.114216","DOIUrl":null,"url":null,"abstract":"<div><div>Betalains are tyrosine-derived plant pigments found exclusively in Caryophyllales and are classified into two groups: red to violet betacyanins and yellow to orange betaxanthins. Betalains show vivid colors and therefore have great potential for flower color modification in ornamental plants. In the present study, we examined the <em>Agrobacterium</em>-mediated genetic transformation of a white-flowered cultivar of <em>Begonia × semperflorens</em> using the RUBY gene, which contains two betalain biosynthesis-related genes (<em>BvDODA1</em> and <em>BvCYP76AD1</em>). The transgenic nature of regenerated plants was confirmed by PCR analysis, and a total of 6 transgenic lines were established. Transgenic plants had pale-pink petals, brownish-olive leaves, ruby-red roots, deep-orange anthers and ruby-red ovules. Transgenic plants showed slightly poor growth compared with wild-type plants. Spectrophotometric analysis showed that both betacyanins and betaxanthins were produced in leaves and petals of transgenic plants. Moreover, the expression level of <em>BvCYP76AD1</em> and <em>BvDODA1</em> in transgenic plants correlated with the amount of betalains. The transgenic <em>Begonia × semperflorens</em> produced in this study with the expression of the RUBY gene effectively demonstrates the validity of genetic transformation, highlighting its potential for enhancing the value of this important ornamental species.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"348 ","pages":"Article 114216"},"PeriodicalIF":4.2000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Betalain-based flower and leaf color modification in Begonia × semperflorens via Agrobacterium-mediated transformation\",\"authors\":\"Ikbal Karatas , Masaru Nakano , Masahiro Otani\",\"doi\":\"10.1016/j.scienta.2025.114216\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Betalains are tyrosine-derived plant pigments found exclusively in Caryophyllales and are classified into two groups: red to violet betacyanins and yellow to orange betaxanthins. Betalains show vivid colors and therefore have great potential for flower color modification in ornamental plants. In the present study, we examined the <em>Agrobacterium</em>-mediated genetic transformation of a white-flowered cultivar of <em>Begonia × semperflorens</em> using the RUBY gene, which contains two betalain biosynthesis-related genes (<em>BvDODA1</em> and <em>BvCYP76AD1</em>). The transgenic nature of regenerated plants was confirmed by PCR analysis, and a total of 6 transgenic lines were established. Transgenic plants had pale-pink petals, brownish-olive leaves, ruby-red roots, deep-orange anthers and ruby-red ovules. Transgenic plants showed slightly poor growth compared with wild-type plants. Spectrophotometric analysis showed that both betacyanins and betaxanthins were produced in leaves and petals of transgenic plants. Moreover, the expression level of <em>BvCYP76AD1</em> and <em>BvDODA1</em> in transgenic plants correlated with the amount of betalains. The transgenic <em>Begonia × semperflorens</em> produced in this study with the expression of the RUBY gene effectively demonstrates the validity of genetic transformation, highlighting its potential for enhancing the value of this important ornamental species.</div></div>\",\"PeriodicalId\":21679,\"journal\":{\"name\":\"Scientia Horticulturae\",\"volume\":\"348 \",\"pages\":\"Article 114216\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientia Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0304423825002651\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304423825002651","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0
摘要
甜菜素是一种酪氨酸衍生的植物色素,仅存在于石竹属植物中,分为两类:红色至紫色甜菜青素和黄色至橙色甜菜青素。Betalains具有鲜艳的色彩,因此在观赏植物的花色修饰方面具有很大的潜力。在本研究中,我们研究了农杆菌介导的白花品种秋海棠(Begonia x semperflorens)利用RUBY基因的遗传转化,该基因含有两个甜菜素生物合成相关基因(BvDODA1和BvCYP76AD1)。通过PCR分析证实了再生植株的转基因性质,共建立了6个转基因品系。转基因植物有浅粉色的花瓣,棕橄榄色的叶子,红宝石红色的根,深橙色的花药和红宝石红色的胚珠。与野生型植物相比,转基因植物的生长稍差。分光光度法分析表明,转基因植株的叶片和花瓣中均可产生甜菜青素和甜菜黄素。此外,BvCYP76AD1和BvDODA1在转基因植株中的表达水平与甜菜素的含量相关。本研究获得的表达RUBY基因的转基因秋海棠有效地证明了遗传转化的有效性,凸显了其在提高这一重要观赏物种价值方面的潜力。
Betalain-based flower and leaf color modification in Begonia × semperflorens via Agrobacterium-mediated transformation
Betalains are tyrosine-derived plant pigments found exclusively in Caryophyllales and are classified into two groups: red to violet betacyanins and yellow to orange betaxanthins. Betalains show vivid colors and therefore have great potential for flower color modification in ornamental plants. In the present study, we examined the Agrobacterium-mediated genetic transformation of a white-flowered cultivar of Begonia × semperflorens using the RUBY gene, which contains two betalain biosynthesis-related genes (BvDODA1 and BvCYP76AD1). The transgenic nature of regenerated plants was confirmed by PCR analysis, and a total of 6 transgenic lines were established. Transgenic plants had pale-pink petals, brownish-olive leaves, ruby-red roots, deep-orange anthers and ruby-red ovules. Transgenic plants showed slightly poor growth compared with wild-type plants. Spectrophotometric analysis showed that both betacyanins and betaxanthins were produced in leaves and petals of transgenic plants. Moreover, the expression level of BvCYP76AD1 and BvDODA1 in transgenic plants correlated with the amount of betalains. The transgenic Begonia × semperflorens produced in this study with the expression of the RUBY gene effectively demonstrates the validity of genetic transformation, highlighting its potential for enhancing the value of this important ornamental species.
期刊介绍:
Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.