金-铂纳米酶免疫层析试纸条快速检测鸡和鱼中氧氟沙星

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2025-06-05 DOI:10.1016/j.jfca.2025.107888

Xuhui Yue , Hongxia Zhang , Jiangxiong Zhu , Shaokang Zhang, Naifeng Xu, Yuanfeng Wang

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引用次数: 0

摘要

氧氟沙星（Ofloxacin， OFL）是第三代喹诺酮类抗生素，据报道在人体残留摄入后会引起不良的多系统反应。本研究的目的是通过逐层种子生长法开发一种用于快速检测OFL的金核-铂壳纳米酶（Au@Pt NPs）。合成的Au@Pt NPs具有25 nm的金核和6个铂壳，具有较强的过氧化物酶样活性。动力学分析表明，该催化剂的米切里斯常数（Km）为0.41 ± 0.04，催化常数（Kcat）为1.41 × 105，催化效率（Kcat/Km）为3.40 × 105。建立了基于这些纳米酶的间接竞争性酶联免疫吸附测定（ic-ELISA）和免疫层析条带。该酶联免疫吸附试验的线性范围为0.2 ~ 7.35 ng/mL， IC50为0.96 ng/mL，检出限（LOD, IC10）为0.03 ng/mL。免疫层析条检测范围为0.060 ~ 4.512 ng/mL， IC50为0.521 ng/mL， LOD为0.017 ng/mL，检测时间为5 ~ 7 min。应用于实际样品，鱼的回收率为86.48 % ~ 116.96 %，鸡的回收率为73.90 % ~ 123.37 %，相对标准偏差（rsd）小于20 %。因此，这种Au@Pt np为基础的免疫分析证明了一种快速、敏感和特异性的方法筛选食品中的抗生素残留。

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A gold-platinum nanozyme-based immunochromatographic strip for rapid detection of ofloxacin in chicken and fish

Ofloxacin (OFL), a third-generation quinolone antibiotic, has been reported to cause adverse multisystem reactions in humans after residual ingestion. The aim of this study was to develop a gold core-platinum shell nanozymes (Au@Pt NPs) via a layer-by-layer seed growth method for the rapid detection of OFL. The synthesized Au@Pt NPs, with a 25 nm gold core and 6 platinum shells, exhibited robust peroxidase-like activity. Kinetic analysis revealed a Michaelis constant (Km) of 0.41 ± 0.04, a catalytic constant (Kcat) of 1.41 × 10⁵, and a catalytic efficiency (Kcat/Km) of 3.40 × 10⁵. An indirect competitive Enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip were developed based on these nanozymes. The ic-ELISA demonstrated a linear range of 0.2–7.35 ng/mL, an IC₅₀ of 0.96 ng/mL, and a limit of detection (LOD, IC₁₀) of 0.03 ng/mL. The immunochromatographic strip exhibited a detection range of 0.060–4.512 ng/mL, an IC₅₀ of 0.521 ng/mL, and an LOD of 0.017 ng/mL, with a detection time of 5–7 min. Applied to real samples, the method achieved recoveries of 86.48 %-116.96 % in fish and 73.90 %-123.37 % in chicken, with relative standard deviations (RSDs) below 20 %. Therefore, this Au@Pt NP-based immunoassay demonstrated a rapid, sensitive, and specific method for screening antibiotic residues in food.

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.