萝卜硫素通过JAK2/STAT3信号上调Prl2c2转录促进骨骼肌损伤后再生

IF 6.2 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Shuang Li, Hong Yu, Huaixin Teng, Lu Zhang, Rui Li* and Huili Tong*, 
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引用次数: 0

摘要

萝卜硫素(Sulforaphane, SFN)是一种亲脂性小分子化合物,进入人体后可被迅速完全吸收。由于其作为抗衰老、抗癌、抗糖尿病和抗菌剂的潜力,它已经获得了广泛的研究关注。然而,SFN在骨骼肌损伤后再生中的作用和机制尚未见报道。本研究表明,SFN可促进骨骼肌损伤后的再生,上调小鼠C2C12成肌细胞的增殖。rna转录组测序数据显示,SFN增加了Prl2C2转录和JAK/STAT信号通路的活性。CHIP和双荧光素酶报告基因实验证实STAT3与Prl2C2启动子结合并调控其转录。因此,SFN影响JAK2/STAT3信号转导活性。最后,通过添加JAK2抑制剂和SFN检测Prl2C2的转录和小鼠C2C12成肌细胞的增殖。结果表明,JAK2抑制剂阻断SFN上调Prl2C2转录和小鼠C2C12成肌细胞增殖。这一现象的发现及其机制为骨骼肌损伤的治疗和动物营养研究提供了指导。SFN在肌肉修复中显示出巨大的潜力,未来的临床试验可以证实其安全性和有效性,为新的基于SFN的治疗铺平道路,为患者提供新的选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Sulforaphane Promotes the Skeletal Muscle Postinjury Regeneration by Up-Regulating the Transcription of Prl2c2 through JAK2/STAT3 Signaling

Sulforaphane Promotes the Skeletal Muscle Postinjury Regeneration by Up-Regulating the Transcription of Prl2c2 through JAK2/STAT3 Signaling

Sulforaphane (SFN), a lipophilic small-molecule compound, can be rapidly and completely absorbed upon entering the body. It has garnered extensive research attention for its potential as an antiaging, anticancer, antidiabetic, and antibacterial agent. However, its role and mechanisms of SFN on skeletal muscle postinjury regeneration have not been reported. This research demonstrated that SFN enhanced the regeneration after skeletal muscle injury and up-regulated the proliferation of mouse C2C12 myoblasts. RNA-transcriptome sequencing data revealed that SFN increased Prl2C2 transcription and JAK/STAT signaling pathway activity. CHIP and dual-luciferase reporter gene assays verified that STAT3 binds to the Prl2C2 promoter and regulates its transcription. Consequently, SFN influenced the JAK2/STAT3 signaling activity. Finally, the transcription of Prl2C2 and the proliferation of mouse C2C12 myoblasts were detected by adding JAK2 inhibitor and SFN. The results showed that the JAK2 inhibitor blocked the up-regulation of SFN on the transcription of Prl2C2 and the proliferation of mouse C2C12 myoblasts. The discovery of this phenomenon and its mechanism offer guidance for treating skeletal muscle injuries and supporting animal nutrition research. SFN shows great potential in muscle repair, and future clinical trials could confirm its safety and efficacy, paving the way for new SFN-based treatments and providing new options for patients.

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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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