基于双级联信号放大策略的不同肿瘤细胞Let-7a高灵敏度检测

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Yan Zhang, Lin Liu, Chun Mei Li, Cheng Zhi Huang* and Jingtao Huang*, 
{"title":"基于双级联信号放大策略的不同肿瘤细胞Let-7a高灵敏度检测","authors":"Yan Zhang,&nbsp;Lin Liu,&nbsp;Chun Mei Li,&nbsp;Cheng Zhi Huang* and Jingtao Huang*,&nbsp;","doi":"10.1021/acs.analchem.5c0086510.1021/acs.analchem.5c00865","DOIUrl":null,"url":null,"abstract":"<p >Let-7a is a key microRNA (miRNA) cancer biomarker closely associated with cancer diagnosis and treatment, but its detection is challenging due to its small size and low abundance. In this study, we developed a highly sensitive cascade amplification strategy by integrating catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) to achieve dual-signal amplification. Two functionalized DNA tetrahedron (TDN) probes, TDN-1 and TDN-2 were first assembled by annealing four sequences and modified with four hairpins. Upon activation by let-7a, a cascade reaction occurred through the TDN-1 and TDN-2 probes, generating DNA aggregates through an isothermal CHA-HCR process. Spatial confinement effect played a key role during the CHA-HCR, enabling highly sensitive detection of let-7a while yielding a detection limit of 8.51 pM. This TDN-CHA-HCR reaction was then successfully applied to in situ imaging of endogenous let-7a in living cells, showing great promise in the early diagnosis and treatment of cancers.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 21","pages":"11182–11189 11182–11189"},"PeriodicalIF":6.7000,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Highly Sensitive Detection of Let-7a in Different Tumor Cells Based on a Dual Cascade Signal Amplification Strategy\",\"authors\":\"Yan Zhang,&nbsp;Lin Liu,&nbsp;Chun Mei Li,&nbsp;Cheng Zhi Huang* and Jingtao Huang*,&nbsp;\",\"doi\":\"10.1021/acs.analchem.5c0086510.1021/acs.analchem.5c00865\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Let-7a is a key microRNA (miRNA) cancer biomarker closely associated with cancer diagnosis and treatment, but its detection is challenging due to its small size and low abundance. In this study, we developed a highly sensitive cascade amplification strategy by integrating catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) to achieve dual-signal amplification. Two functionalized DNA tetrahedron (TDN) probes, TDN-1 and TDN-2 were first assembled by annealing four sequences and modified with four hairpins. Upon activation by let-7a, a cascade reaction occurred through the TDN-1 and TDN-2 probes, generating DNA aggregates through an isothermal CHA-HCR process. Spatial confinement effect played a key role during the CHA-HCR, enabling highly sensitive detection of let-7a while yielding a detection limit of 8.51 pM. This TDN-CHA-HCR reaction was then successfully applied to in situ imaging of endogenous let-7a in living cells, showing great promise in the early diagnosis and treatment of cancers.</p>\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"97 21\",\"pages\":\"11182–11189 11182–11189\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2025-05-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.analchem.5c00865\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.analchem.5c00865","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

摘要

Let-7a是一种与癌症诊断和治疗密切相关的关键microRNA (miRNA)癌症生物标志物,但由于其体积小且丰度低,其检测具有挑战性。在这项研究中,我们开发了一种高灵敏度的级联扩增策略,通过整合催化发夹组装(CHA)和杂交链反应(HCR)来实现双信号扩增。首先用4个序列退火组装了两个功能化的DNA四面体(TDN)探针TDN-1和TDN-2,并用4个发夹修饰。被let-7a激活后,通过TDN-1和TDN-2探针发生级联反应,通过等温CHA-HCR过程产生DNA聚集体。空间约束效应在CHA-HCR过程中发挥了关键作用,使let-7a的检测灵敏度高,检测限为8.51 pM。该TDN-CHA-HCR反应随后成功应用于活细胞内源性let-7a的原位成像,在癌症的早期诊断和治疗中显示出巨大的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Highly Sensitive Detection of Let-7a in Different Tumor Cells Based on a Dual Cascade Signal Amplification Strategy

Highly Sensitive Detection of Let-7a in Different Tumor Cells Based on a Dual Cascade Signal Amplification Strategy

Let-7a is a key microRNA (miRNA) cancer biomarker closely associated with cancer diagnosis and treatment, but its detection is challenging due to its small size and low abundance. In this study, we developed a highly sensitive cascade amplification strategy by integrating catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) to achieve dual-signal amplification. Two functionalized DNA tetrahedron (TDN) probes, TDN-1 and TDN-2 were first assembled by annealing four sequences and modified with four hairpins. Upon activation by let-7a, a cascade reaction occurred through the TDN-1 and TDN-2 probes, generating DNA aggregates through an isothermal CHA-HCR process. Spatial confinement effect played a key role during the CHA-HCR, enabling highly sensitive detection of let-7a while yielding a detection limit of 8.51 pM. This TDN-CHA-HCR reaction was then successfully applied to in situ imaging of endogenous let-7a in living cells, showing great promise in the early diagnosis and treatment of cancers.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信