Yan Zhang, Lin Liu, Chun Mei Li, Cheng Zhi Huang* and Jingtao Huang*,
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Highly Sensitive Detection of Let-7a in Different Tumor Cells Based on a Dual Cascade Signal Amplification Strategy
Let-7a is a key microRNA (miRNA) cancer biomarker closely associated with cancer diagnosis and treatment, but its detection is challenging due to its small size and low abundance. In this study, we developed a highly sensitive cascade amplification strategy by integrating catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) to achieve dual-signal amplification. Two functionalized DNA tetrahedron (TDN) probes, TDN-1 and TDN-2 were first assembled by annealing four sequences and modified with four hairpins. Upon activation by let-7a, a cascade reaction occurred through the TDN-1 and TDN-2 probes, generating DNA aggregates through an isothermal CHA-HCR process. Spatial confinement effect played a key role during the CHA-HCR, enabling highly sensitive detection of let-7a while yielding a detection limit of 8.51 pM. This TDN-CHA-HCR reaction was then successfully applied to in situ imaging of endogenous let-7a in living cells, showing great promise in the early diagnosis and treatment of cancers.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.