用水滴萃取和无接触电喷雾电离质谱法无损监测生物体的代谢。

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-05-26 DOI:10.1021/acs.analchem.5c01018

Yuanyi He,Xiaojie Chen,Lecheng Lyu,Richard N Zare,Guangming Huang

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引用次数: 0

摘要

我们提出了一种新的工具，用于非破坏性体内代谢物分析，我们称之为MetaTube。这种方法利用高电压来促进有效的电离，同时保持与被取样的活组织的电隔离。MetaTube在代谢物信号强度方面实现了2-3个数量级的提高，使MetaTube能够识别10倍以上的代谢物。这些能力使我们能够分析人体皮肤表面的代谢物，并监测生长中的细菌菌落的代谢物波动。MetaTube为了解体内代谢动力学和实时评估活组织的代谢状态提供了一种很有前途的策略。

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Nondestructive Metabolic Monitoring of Living Organisms by Water-Droplet Extraction and Contact-Free Electrospray Ionization Mass Spectrometry.

We present a novel tool for nondestructive in vivo metabolite analysis, which we call MetaTube. This method utilizes a high voltage to facilitate efficient ionization while maintaining electrical isolation from living tissues that are sampled. MetaTube achieves a 2-3 order of magnitude improvement in metabolite signal intensity, giving MetaTube the capacity to identify 10-fold more metabolites. These capabilities allow us to profile metabolites of human skin surfaces and monitor metabolite fluctuations of growing bacterial colonies. MetaTube offers a promising strategy to understand in vivo metabolic dynamics and evaluate the metabolic state of living tissues in real time.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.