11种超级水果花青素提取物可减轻镉暴露诱导的HK-2细胞氧化应激和炎症损伤

IF 6.9 Q1 FOOD SCIENCE & TECHNOLOGY
Food frontiers Pub Date : 2025-04-09 DOI:10.1002/fft2.70019
Jiaojiao Liang, Yunyi Chen, Manxi Wu, Jiebiao Chen, Cui Sun, Jinping Cao, Yue Wang, Chongde Sun
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引用次数: 0

摘要

镉(Cd)污染已成为对人类健康日益严重的威胁,其引起的肾毒性与氧化应激和炎症密切相关。本研究利用uplc -三重tof /MS对11种超级水果中的初级花青素进行了提取和分析,并通过DPPH、ABTS、FRAP和ORAC检测对其抗氧化能力进行了评价。采用cd暴露的人肾-2 (HK-2)细胞损伤模型,研究超果花青素提取物的保护作用。结果表明,这些提取物可提高HK-2细胞活力,提高T-SOD和GSH水平,降低ROS、MDA和NO。此外,它们显著调节氧化应激和炎症相关基因和蛋白质。此外,我们阐明了这些花青素通过经典的氧化和炎症途径减轻cd诱导的细胞损伤的机制。这些发现强调了富含花青素的特定水果在减少cd相关健康风险方面的潜力,并建议其生物活性特性在促进人类健康方面的实际应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK-2 Cells Induced by Cadmium Exposure

Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK-2 Cells Induced by Cadmium Exposure

Cadmium (Cd) pollution has emerged as an escalating threat to human health, with its induced kidney toxicity being closely linked to oxidative stress and inflammation. In this study, we extracted and analyzed primary anthocyanins from 11 superfruits using UPLC-Triple-TOF/MS and evaluated their antioxidant capacity through DPPH, ABTS, FRAP, and ORAC assays. By employing a Cd-exposed Human Kidney-2 (HK-2) cells injury model, we investigated the protective effects of superfruit anthocyanin extracts. The results demonstrated that these extracts enhanced HK-2 cell viability, increased T-SOD and GSH levels, and reduced ROS, MDA, and NO. Furthermore, they significantly regulated oxidative stress and inflammation-related genes and proteins. Moreover, we elucidated the mechanisms by which these anthocyanins mitigate Cd-induced cellular damage via classical oxidative and inflammatory pathways. These findings highlight the potential of specific anthocyanin-rich fruits in reducing Cd-related health risks and suggest practical applications for their bioactive properties in promoting human health.

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