自噬和头发过早变白:LC3作为一种生物标志物在病例对照研究中的作用

IF 2.3 4区 医学 Q2 DERMATOLOGY
Wafaa Mohamed Abd-Elmagid, Khalda S Amr, Hoda A Ahmed, Dina Ali, Amr Abdelhamed
{"title":"自噬和头发过早变白:LC3作为一种生物标志物在病例对照研究中的作用","authors":"Wafaa Mohamed Abd-Elmagid, Khalda S Amr, Hoda A Ahmed, Dina Ali, Amr Abdelhamed","doi":"10.5826/dpc.1502a4876","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Premature graying of hair (PGH) is a common disorder with a multifactorial etiology. Autophagy, which is self-cellular digestion, has been linked to melanin pigment formation; however, the role of autophagy in PGH has not been investigated well.</p><p><strong>Objectives: </strong>The study aimed to evaluate the relationship between PGH and autophagy by measuring gene expression and serum microtubule-associated protein light chain 3 (LC3) concentration.</p><p><strong>Methods: </strong>A case-control study was conducted on 39 PGH patients and 21 controls. Patients clinically diagnosed with PGH and aged <30 years were included in the study. Blood samples were taken to detect LC3B protein by ELISA in the serum of both groups. White hairs from both groups were collected to detect LC3B gene expression by PCR.</p><p><strong>Results: </strong>There was a statistically significant difference between the two groups as regards expression levels of the LC3 gene by PCR (P<0.001), with the mean in the control group (0.71± 0.3) lower than in the PGH group (5.1 ± 1.4). Also, there was a positive significant correlation between LC3 concentration and LC3 gene expression in control (r=0.867, P< 0.001) and in PGH patients (r=0.954, P≤0.001). Multivariate logistic regression analysis for PGH predictors using age, sex (female), hemoglobin level, LC3 concentration, and LC3 gene expression revealed that the only predictor of PGH was LC3 gene expression.</p><p><strong>Conclusions: </strong>Premature graying of hair may have a link with autophagy. LC3 gene expression was increased in PGH patients as compared to the control. LC3 gene expression may be an independent predictor of PGH development. Autophagy modulation may be a therapeutic target for PGH.</p>","PeriodicalId":11168,"journal":{"name":"Dermatology practical & conceptual","volume":"15 2","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12090954/pdf/","citationCount":"0","resultStr":"{\"title\":\"Autophagy and Premature Graying of Hair: The Role of LC3 as a Biomarker in a Case-Control Study.\",\"authors\":\"Wafaa Mohamed Abd-Elmagid, Khalda S Amr, Hoda A Ahmed, Dina Ali, Amr Abdelhamed\",\"doi\":\"10.5826/dpc.1502a4876\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Premature graying of hair (PGH) is a common disorder with a multifactorial etiology. Autophagy, which is self-cellular digestion, has been linked to melanin pigment formation; however, the role of autophagy in PGH has not been investigated well.</p><p><strong>Objectives: </strong>The study aimed to evaluate the relationship between PGH and autophagy by measuring gene expression and serum microtubule-associated protein light chain 3 (LC3) concentration.</p><p><strong>Methods: </strong>A case-control study was conducted on 39 PGH patients and 21 controls. Patients clinically diagnosed with PGH and aged <30 years were included in the study. Blood samples were taken to detect LC3B protein by ELISA in the serum of both groups. White hairs from both groups were collected to detect LC3B gene expression by PCR.</p><p><strong>Results: </strong>There was a statistically significant difference between the two groups as regards expression levels of the LC3 gene by PCR (P<0.001), with the mean in the control group (0.71± 0.3) lower than in the PGH group (5.1 ± 1.4). Also, there was a positive significant correlation between LC3 concentration and LC3 gene expression in control (r=0.867, P< 0.001) and in PGH patients (r=0.954, P≤0.001). Multivariate logistic regression analysis for PGH predictors using age, sex (female), hemoglobin level, LC3 concentration, and LC3 gene expression revealed that the only predictor of PGH was LC3 gene expression.</p><p><strong>Conclusions: </strong>Premature graying of hair may have a link with autophagy. LC3 gene expression was increased in PGH patients as compared to the control. LC3 gene expression may be an independent predictor of PGH development. Autophagy modulation may be a therapeutic target for PGH.</p>\",\"PeriodicalId\":11168,\"journal\":{\"name\":\"Dermatology practical & conceptual\",\"volume\":\"15 2\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12090954/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Dermatology practical & conceptual\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.5826/dpc.1502a4876\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dermatology practical & conceptual","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5826/dpc.1502a4876","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

简介:头发过早变白(PGH)是一种常见的疾病与多因素的病因。自噬是一种自我细胞消化,与黑色素的形成有关;然而,自噬在PGH中的作用尚未得到很好的研究。目的:通过检测基因表达和血清微管相关蛋白轻链3 (LC3)浓度,探讨PGH与自噬的关系。方法:对39例PGH患者和21例对照组进行病例对照研究。临床诊断为PGH与老年患者结果:两组LC3基因PCR表达水平差异有统计学意义(p)结论:头发过早变白可能与自噬有关。与对照组相比,PGH患者的LC3基因表达增加。LC3基因表达可能是PGH发展的独立预测因子。自噬调节可能是PGH的一个治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Autophagy and Premature Graying of Hair: The Role of LC3 as a Biomarker in a Case-Control Study.

Autophagy and Premature Graying of Hair: The Role of LC3 as a Biomarker in a Case-Control Study.

Autophagy and Premature Graying of Hair: The Role of LC3 as a Biomarker in a Case-Control Study.

Autophagy and Premature Graying of Hair: The Role of LC3 as a Biomarker in a Case-Control Study.

Introduction: Premature graying of hair (PGH) is a common disorder with a multifactorial etiology. Autophagy, which is self-cellular digestion, has been linked to melanin pigment formation; however, the role of autophagy in PGH has not been investigated well.

Objectives: The study aimed to evaluate the relationship between PGH and autophagy by measuring gene expression and serum microtubule-associated protein light chain 3 (LC3) concentration.

Methods: A case-control study was conducted on 39 PGH patients and 21 controls. Patients clinically diagnosed with PGH and aged <30 years were included in the study. Blood samples were taken to detect LC3B protein by ELISA in the serum of both groups. White hairs from both groups were collected to detect LC3B gene expression by PCR.

Results: There was a statistically significant difference between the two groups as regards expression levels of the LC3 gene by PCR (P<0.001), with the mean in the control group (0.71± 0.3) lower than in the PGH group (5.1 ± 1.4). Also, there was a positive significant correlation between LC3 concentration and LC3 gene expression in control (r=0.867, P< 0.001) and in PGH patients (r=0.954, P≤0.001). Multivariate logistic regression analysis for PGH predictors using age, sex (female), hemoglobin level, LC3 concentration, and LC3 gene expression revealed that the only predictor of PGH was LC3 gene expression.

Conclusions: Premature graying of hair may have a link with autophagy. LC3 gene expression was increased in PGH patients as compared to the control. LC3 gene expression may be an independent predictor of PGH development. Autophagy modulation may be a therapeutic target for PGH.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.40
自引率
0.00%
发文量
217
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信