Bin Liu, Yingying Wan, Miaohuan Xiong, Jialu Li, Huipeng Wu, Xuelan Chen, Jian He
{"title":"Metabacillus sp. JX24中转化除草剂Mesotrione的新型硝基还原酶的鉴定与表征","authors":"Bin Liu, Yingying Wan, Miaohuan Xiong, Jialu Li, Huipeng Wu, Xuelan Chen, Jian He","doi":"10.1021/acs.jafc.4c11559","DOIUrl":null,"url":null,"abstract":"Mesotrione, a commonly used 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicide, threatens agro-ecosystem sustainability and nontarget organisms. Microbes play a significant role in the biodegradation of mesotrione. However, an understanding of the molecular mechanisms involved in mesotrione degradation is still quite limited. Here, a bacterial strain <i>Metabacillus</i> sp. JX24 capable of degrading mesotrione was isolated. The strain could effectively degrade about 98% of 100 μM mesotrione within 12 h. It was revealed that mesotrione was degraded through successive reduction of its nitro group to generate 2-amino-4-methylsulfonylbenzoic acid (AMBA). A gene, <i>mnrA</i>, encoding a novel nitroreductase MnrA responsible for the transformation of mesotrione, was cloned from strain JX24. MnrA shared low sequence identities (<20%) with the reported mesotrione nitroreductases. The purified MnrA catalyzed the nitro reduction of mesotrione with NADH or NADPH as cofactors in vitro. The <i>K</i><sub>m</sub> and <i>k</i><sub>cat</sub>/<i>K</i><sub>m</sub> values of MnrA for mesotrione were 22.0 μM and 0.57 μM<sup>–1</sup> min<sup>–1</sup>, respectively. The detoxification assay indicated that AMBA, the product of MnrA-mediated mesotrione reduction, did not inhibit HPPD activity, suggesting that MnrA confers a detoxifying action on mesotrione. Our work provides a novel enzymatic resource for the elimination of mesotrione residues in the environment.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"34 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification and Characterization of a Novel Nitroreductase Transforming the Herbicide Mesotrione in Metabacillus sp. JX24\",\"authors\":\"Bin Liu, Yingying Wan, Miaohuan Xiong, Jialu Li, Huipeng Wu, Xuelan Chen, Jian He\",\"doi\":\"10.1021/acs.jafc.4c11559\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Mesotrione, a commonly used 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicide, threatens agro-ecosystem sustainability and nontarget organisms. Microbes play a significant role in the biodegradation of mesotrione. However, an understanding of the molecular mechanisms involved in mesotrione degradation is still quite limited. Here, a bacterial strain <i>Metabacillus</i> sp. JX24 capable of degrading mesotrione was isolated. The strain could effectively degrade about 98% of 100 μM mesotrione within 12 h. It was revealed that mesotrione was degraded through successive reduction of its nitro group to generate 2-amino-4-methylsulfonylbenzoic acid (AMBA). A gene, <i>mnrA</i>, encoding a novel nitroreductase MnrA responsible for the transformation of mesotrione, was cloned from strain JX24. MnrA shared low sequence identities (<20%) with the reported mesotrione nitroreductases. The purified MnrA catalyzed the nitro reduction of mesotrione with NADH or NADPH as cofactors in vitro. The <i>K</i><sub>m</sub> and <i>k</i><sub>cat</sub>/<i>K</i><sub>m</sub> values of MnrA for mesotrione were 22.0 μM and 0.57 μM<sup>–1</sup> min<sup>–1</sup>, respectively. The detoxification assay indicated that AMBA, the product of MnrA-mediated mesotrione reduction, did not inhibit HPPD activity, suggesting that MnrA confers a detoxifying action on mesotrione. Our work provides a novel enzymatic resource for the elimination of mesotrione residues in the environment.\",\"PeriodicalId\":41,\"journal\":{\"name\":\"Journal of Agricultural and Food Chemistry\",\"volume\":\"34 1\",\"pages\":\"\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-05-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Agricultural and Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.jafc.4c11559\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1021/acs.jafc.4c11559","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Identification and Characterization of a Novel Nitroreductase Transforming the Herbicide Mesotrione in Metabacillus sp. JX24
Mesotrione, a commonly used 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicide, threatens agro-ecosystem sustainability and nontarget organisms. Microbes play a significant role in the biodegradation of mesotrione. However, an understanding of the molecular mechanisms involved in mesotrione degradation is still quite limited. Here, a bacterial strain Metabacillus sp. JX24 capable of degrading mesotrione was isolated. The strain could effectively degrade about 98% of 100 μM mesotrione within 12 h. It was revealed that mesotrione was degraded through successive reduction of its nitro group to generate 2-amino-4-methylsulfonylbenzoic acid (AMBA). A gene, mnrA, encoding a novel nitroreductase MnrA responsible for the transformation of mesotrione, was cloned from strain JX24. MnrA shared low sequence identities (<20%) with the reported mesotrione nitroreductases. The purified MnrA catalyzed the nitro reduction of mesotrione with NADH or NADPH as cofactors in vitro. The Km and kcat/Km values of MnrA for mesotrione were 22.0 μM and 0.57 μM–1 min–1, respectively. The detoxification assay indicated that AMBA, the product of MnrA-mediated mesotrione reduction, did not inhibit HPPD activity, suggesting that MnrA confers a detoxifying action on mesotrione. Our work provides a novel enzymatic resource for the elimination of mesotrione residues in the environment.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.