{"title":"西洋参茎叶果胶多糖的制备、结构特征及对心肌肥厚的抑制作用。","authors":"Minghui Zhang, Yu Xu, Lei Zhang, Peipei Wang","doi":"10.1002/pca.3535","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Panacis Quinquefolii Radix, a valued herb in traditional Chinese medicine, is frequently prescribed for its immunomodulatory effects and its potential to improve cardiovascular function. Recently, there is an increasing amount of research on ginseng polysaccharides, due to their variety of pharmacological activities, including anti-inflammatory, hepatoprotective, cardiovascular protective, and the improvement of intestinal function.</p><p><strong>Objective: </strong>This study investigates the efficacy of Panacis Quinquefolii Radix polysaccharides in alleviating cardiovascular diseases and to achieve the high-value utilization of ginseng by-products.</p><p><strong>Methodology: </strong>A pectic polysaccharide named XYSJY2-2 was extracted with hot water from stems and leaves of Panacis Quinquefolii Radix and further isolated by a DEAE Sepharose Fast Flow column and a Sephacry S-100 column. The structural characteristics of XYSJY2-2 was determined by monosaccharide composition, IR, NMR, and methylation analysis. The cardioprotective activity of this polysaccharide was evaluated in vitro and in vivo. With a relative molecular weight of 5.1 kDa, XYSJY2-2 is a type I rhamnogalacturonan featuring a core backbone made up of alternately linked rhamnose and galacturonic acid.</p><p><strong>Results: </strong>XYSJY2-2 effectively alleviated myocardial hypertrophy and remodeling induced by Ang II and TAC in vivo and in vitro, partly due to suppressing Nppa and Nppb expression.</p><p><strong>Conclusion: </strong>A pectic polysaccharide from stems and leaves of Panacis Quinquefolii Radix has the potential to alleviate cardiovascular disease.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Pectic Polysaccharide From Stems and Leaves of Panacis Quinquefolii Radix: Preparation, Structural Characteristic, and Inhibition of Cardiac Hypertrophy.\",\"authors\":\"Minghui Zhang, Yu Xu, Lei Zhang, Peipei Wang\",\"doi\":\"10.1002/pca.3535\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Panacis Quinquefolii Radix, a valued herb in traditional Chinese medicine, is frequently prescribed for its immunomodulatory effects and its potential to improve cardiovascular function. 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引用次数: 0
摘要
摘要：西洋参是一种珍贵的中药，因其具有免疫调节作用和改善心血管功能的潜力而经常被处方。近年来，由于人参多糖具有抗炎、保肝、保心血管、改善肠道功能等多种药理作用，对人参多糖的研究越来越多。目的：研究西洋参多糖对心血管疾病的缓解作用，实现人参副产物的高价值利用。方法：用热水从西洋参茎叶中提取果胶多糖XYSJY2-2，采用DEAE Sepharose Fast Flow柱和Sephacry S-100柱进行分离。通过单糖组成、红外光谱、核磁共振和甲基化分析确定了XYSJY2-2的结构特征。体外和体内评价了该多糖的心脏保护活性。XYSJY2-2是一种I型鼠李糖半乳糖醛酸，相对分子量为5.1 kDa，核心骨架由鼠李糖和半乳糖醛酸交替连接而成。结果：XYSJY2-2在体内外均能有效减轻Ang II和TAC诱导的心肌肥大和重构，其部分原因是抑制Nppa和Nppb的表达。结论：西洋参茎叶果胶多糖具有缓解心血管疾病的作用。
A Pectic Polysaccharide From Stems and Leaves of Panacis Quinquefolii Radix: Preparation, Structural Characteristic, and Inhibition of Cardiac Hypertrophy.
Introduction: Panacis Quinquefolii Radix, a valued herb in traditional Chinese medicine, is frequently prescribed for its immunomodulatory effects and its potential to improve cardiovascular function. Recently, there is an increasing amount of research on ginseng polysaccharides, due to their variety of pharmacological activities, including anti-inflammatory, hepatoprotective, cardiovascular protective, and the improvement of intestinal function.
Objective: This study investigates the efficacy of Panacis Quinquefolii Radix polysaccharides in alleviating cardiovascular diseases and to achieve the high-value utilization of ginseng by-products.
Methodology: A pectic polysaccharide named XYSJY2-2 was extracted with hot water from stems and leaves of Panacis Quinquefolii Radix and further isolated by a DEAE Sepharose Fast Flow column and a Sephacry S-100 column. The structural characteristics of XYSJY2-2 was determined by monosaccharide composition, IR, NMR, and methylation analysis. The cardioprotective activity of this polysaccharide was evaluated in vitro and in vivo. With a relative molecular weight of 5.1 kDa, XYSJY2-2 is a type I rhamnogalacturonan featuring a core backbone made up of alternately linked rhamnose and galacturonic acid.
Results: XYSJY2-2 effectively alleviated myocardial hypertrophy and remodeling induced by Ang II and TAC in vivo and in vitro, partly due to suppressing Nppa and Nppb expression.
Conclusion: A pectic polysaccharide from stems and leaves of Panacis Quinquefolii Radix has the potential to alleviate cardiovascular disease.
期刊介绍：
Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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