农业研究中快速、简单、方便的植物营养评价方法。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-05-13 DOI:10.1002/jsfa.14363

Zipora Tietel, Izabella Galilov, Sarit Melamed

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引用次数: 0

摘要

背景：农业研究在研究遗传背景和环境条件对植物和商业参数的影响方面是充分的。然而，关于营养价值和植物营养成分的影响的数据很少，尽管它对消费者很重要，在提供营养安全方面也很重要。本研究旨在简化、统一和提供一套分光光度法营养评价方法，为农业研究提供一个有价值的工具。努力提供简单、快速、小规模和具有成本效益的协议，目的是尽量减少资源和时间、学习曲线和错误。结果：我们纳入了抗氧化能力（ABTS， DPPH， FRAP），多酚和黄酮类化合物（游离，结合，结合，总），木脂素，花青素，生育酚，植物甾醇，硫代葡萄糖苷，类胡萝卜素，β素，皂苷，绿单宁，藻绿胆素，维生素C，总油和可溶性蛋白含量，以及抗营养物质-草酸和植酸。包括推荐标准的校准曲线，并演示了工作流程。还提供了协议调整的指导方针。结论：因此，这项工作汇编了广泛的严格方法库，提供了良好的营养评估方案，包括营养植物化学成分和基本生物活性评估（抗氧化测量）。这些方法被推荐用于评估农业管理实践的影响，如水和矿物质营养的可用性或采后储存，或作为育种计划的一部分，如研究或商业研发实验室，这些实验室不具备营养分析方面的专业知识，需要可获得的高通量营养评估工具。©2025作者。约翰威利父子有限公司代表化学工业协会出版的《食品与农业科学杂志》。

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Rapid, simple and accessible phytonutritional assessment methods in agricultural research.

Background: Agricultural research with respect to studying the effects of genetic background and environmental conditions on plant and commercial parameters is ample. Nevertheless, data on the effects on nutritional value and phytonutritional composition are scarce, despite its significance to the consumer, as well as its importance in providing nutritional security. The present study aimed to simplify, unify and make accessible a set of spectrophotometric nutritional assessment assays, comprising a valuable tool in agricultural research. Efforts were made to provide simple, rapid, small-scale and cost-effective protocols, aiming to minimize resources and time, learning-curve and erring.

Results: We included assays alongside literature-compliant values for antioxidant capacity (ABTS, DPPH, FRAP), polyphenols and flavonoids (free, conjugated, bound, total), lignans, anthocyanins, tocopherols, phytosterols, glucosinolates, carotenoids, betalains, saponins, phlorotannin, phycobilin, vitamin C, total oil and soluble protein content, in addition to antinutrients- oxalic acid and phytic acid. Calibration curves of recommended standards are included and workflows are demonstrated. Guidelines are also provided for protocol adjustments.

Conclusion: This work thus compiles a broad arsenal of rigorous methods, rendering a good spectrum of nutritional assessment protocols, including nutriphytochemical composition alongside a basic bioactivity assessment (antioxidant measurements). These methods are recommended in evaluating the effects of agricultural management practices, such as water and mineral nutrition availability or postharvest storage, or as part of breeding programs, such as in research or commercial R&D laboratories that do not have expertise in nutritional analysis, and require an accessible high-throughput nutritional assessment tool. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.