花生皮原花青素参与Caco-2细胞glut2 - plc - β2- pkc通路，降低肠道葡萄糖吸收。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-05-12 DOI:10.1002/jsfa.14309

Min Liu, Zhengyao Li, Tingting Ju, Hongli Zhang, Xinlei Peng, Jun Liu

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引用次数: 0

摘要

背景：抑制葡萄糖转运与控制餐后高血糖有关，可降低糖尿病的风险。随着原花青素研究的深入，胃肠道是原花青素发挥积极生物活性的主要有效部位。花生皮原花青素（PSP）已显示出降低血糖水平的潜力，但其在肠道中的潜在机制尚不清楚。本研究的目的是通过体外模型研究PSP对双糖酶活性和葡萄糖转运的影响。结果：PSP对Caco-2细胞的麦芽糖酶有明显的抑制作用。此外，在Caco-2单层模型中，PSP以时间和剂量依赖的方式减少葡萄糖转运，并通过激活磷脂酶C β2/蛋白激酶C信号通路下调葡萄糖转运蛋白2的表达。结论：PSP可能通过调节肠道细胞内葡萄糖转运蛋白和信号通路，减少葡萄糖转运，可能具有良好的降糖作用。©2025化学工业协会。

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Peanut skin procyanidins reduce intestinal glucose absorption involved in GLUT2-PLCβ2-PKC pathway in Caco-2 cells.

Background: Inhibition of glucose transport is associated with controlling postprandial hyperglycemia, which can reduce the risk of diabetes. As the research of procyanidins deepens, the gastrointestinal tract is the major effective site of procyanidins for exhibiting positive bio-activities. Peanut skin procyanidins (PSP) have shown potential in lowering blood glucose level, but the underlying mechanisms in the gut remain unclear. The aim of this study was to examine the effects of PSP on disaccharidase enzyme activity and glucose transport using an in vitro model.

Results: PSP showed a significant inhibiting activity of maltase in Caco-2 cells. Furthermore, PSP reduced glucose transport in a time- and dose-dependent manner in a Caco-2 monolayer model, as well as downregulating glucose transporter 2 expression through the activation of the phospholipase C β2/protein kinase C signaling pathway.

Conclusion: The results indicate that PSP may reduce glucose transport via regulating glucose transporters and modulating the signaling pathway in intestinal cells, potentially offering beneficial antihyperglycemic effects. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.