荧光油酸包封ZnSe/CdS/核壳量子点在Balb/c小鼠体内静脉注射后的生物相容性及生物分布评价

IF 2.2 4区 工程技术 Q3 PHARMACOLOGY & PHARMACY
Bioimpacts Pub Date : 2025-02-17 eCollection Date: 2025-01-01 DOI:10.34172/bi.30467
Aakriti Tyagi, Disha Mittal, S Bhanoth, Ankita Leekha, Anita K Verma
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引用次数: 0

摘要

量子点(QDs)是具有固有荧光的半导体纳米晶体,与传统荧光探针相比,它具有几个优点,包括体积小(5-10 nm),可调谐的激发和发射光谱,易于表面功能化,以及强大的光稳定性,使其成为体内成像的理想候选人。量子点的行为高度依赖于表面功能。量子点在生物系统中的体内毒性是阻碍其应用于临床的主要限制。方法:研究油酸封包的水溶性ZnSe/CdS核心壳QDs对人肝癌细胞系hep3b、人胚胎肾细胞系hek 293和ehrich腹水细胞eac的细胞毒性。为了评估其体内治疗效果,我们对Balb/c小鼠进行了OA封顶ZnSe/ CdS QDs的初步动物毒性研究。以10 nM/kg/200µL/只小鼠为剂量静脉注射QDs 28 d,观察其血清稳定性、药代动力学、生物分布及γ-显像。结果:OA封顶QDs对Hep3B的IC50为29.85µg/mL。通过在Hep3B细胞中释放LDH观察到,QDs的毒性主要是由于活性氧的产生。溶血作用可忽略不计,表明OA封顶的量子点具有生物相容性。OA封顶的QDs主要积聚在肝脏和脾脏,在肾脏无滞留。结论:OA封顶的ZnSe/ CdS量子点对Hep3B和EAC细胞具有较强的抗癌作用。此外,在Balb/c小鼠的重要器官中观察到最小的积累和滞留,保护它们免受潜在的不良副作用,强调了它们在生物医学应用方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of the biocompatibility and biodistribution of fluorescent oleic acid capped ZnSe/CdS/ core shell quantum dots after intravenous injection in Balb/c mice.

Introduction: Quantum dots (QDs) are semiconductor nanocrystals with inherent fluorescence having several advantages over traditional fluorescent probes including their small size (5-10 nm), tunable excitation and emission spectra, ease of surface functionalization, and robust photostability that makes them ideal candidates for in vivo imaging. The behavior of QDs is highly dependent on the surface functionality. In vivo toxicity of QDs in biological systems is the major limitation hindering their translation to clinics.

Methods: In the present study, cytotoxicity of water soluble ZnSe/CdS core shell QDs capped with oleic acid was evaluated against human hepatocellular carcinoma cell line-Hep3B, Human Embryonic Kidney cell line-HEK 293 and Ehlrich Ascitic cells-EAC. To assess its in vivo therapeutic efficacy, the initial animal toxicity studies of OA capped ZnSe/ CdS QDs were done in Balb/c mice. Serum stability, pharmacokinetics, biodistribution and γ-scintigraphic imaging were observed in mice after intravenous (i.v) injection of QDs at a dose of 10 nM/kg/200 µL/mice up to 28 days.

Results: IC50 of OA capped QDs against Hep3B was 29.85 µg/mL at 24 hours. QDs toxicity was primarily due to the generation of reactive oxygen species as observed by LDH release in Hep3B cells. Negligible haemolysis indicated that OA capped QDs were biocompatible. OA capped QDs mainly accumulated in the liver and spleen with no retention in kidneys.

Conclusion: OA capped ZnSe/ CdS QDs exhibited enhanced anti-cancer efficacy against Hep3B and EAC cell line. Further, minimum accumulation and retention were observed in vital organs in Balb/c mice protecting them from potential adverse side effects, underlining their potential for biomedical applications.

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来源期刊
Bioimpacts
Bioimpacts Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
4.80
自引率
7.70%
发文量
36
审稿时长
5 weeks
期刊介绍: BioImpacts (BI) is a peer-reviewed multidisciplinary international journal, covering original research articles, reviews, commentaries, hypotheses, methodologies, and visions/reflections dealing with all aspects of biological and biomedical researches at molecular, cellular, functional and translational dimensions.
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