山药球茎中两种非淀粉多糖的纯化及性质研究。铁根及其抗氧化和降糖活性。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-04-25 DOI:10.1002/jsfa.14296

Li Wang, Yu-Peng Yang, Yi Tian, Shu-Cheng Huang, Yuan Ruan, Chun-Nan Wen, Miao Liu, Bing-Ji Ma

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引用次数: 0

摘要

背景：黄薯蓣。简历。“铁根”具有巨大的农业和经济价值。它的地下块茎被广泛用于食品和传统医药，但它的球茎——叶腋上的小球状结构——经常被忽视和丢弃。这浪费了生物资源，降低了植物的整体生产力。结果：为解决这一问题，采用亚临界水萃取法从球泡中提取物质，并进行分离纯化。得到两种非淀粉多糖组分DBP1和DBP2，其提取率分别为2.25%和0.85%。随后对其性质和活性的研究表明，DBP1 （21.9 kDa）是一种主要由Gal和Glc组成的中性多糖，DBP2 （109.8 kDa）是一种主要由GalA和Gal组成的酸性多糖。由于DBP1的产率较高，因此对其结构的研究更深入。甲基化实验表明，其主链由1,4- galp和1,4,6- glcp糖苷键组成，具有分支点，与核磁共振（NMR）结果一致。扫描电镜显示，DBP1和DBP2具有不同的丝状结构，但具有相似的球形形态。傅里叶变换红外光谱和热分析研究了它们的官能团和热稳定性。在活性试验中，DBP1和DBP2均表现出抗氧化活性，并能抑制α-葡萄糖苷酶和α-淀粉酶，具有降血糖活性。结论：两种非淀粉多糖DBP1和DBP2具有良好的抗氧化和降糖作用。它们可以作为功能性食品和药物的原料，值得进一步开发。最大限度地利用球茎，促进绿色、可持续农业，防止资源浪费。©2025化学工业协会。

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Purification and characterization of two non-starch polysaccharides from bulbils of Dioscorea opposita Thunb. 'Tiegun' and their antioxidant and hypoglycemic activity.

Background: Dioscorea opposita Thunb. cv. 'Tiegun' has substantial agricultural and economic value. Its underground tubers are used widely in food and traditional medicine but the bulbils - small globular structures in the leaf axils - are frequently overlooked and discarded. This wastes biological resources and reduces overall plant productivity.

Results: To address this, subcritical water extraction was applied to extract substances from the bulbils, followed by separation and purification. As a result, two non-starch polysaccharide components, DBP1 and DBP2, were obtained, with extraction rates of 2.25% and 0.85% respectively. Subsequent research on their properties and activity showed that DBP1 (21.9 kDa) was a neutral polysaccharide mainly made of Gal and Glc, and DBP2 (109.8 kDa) was an acidic polysaccharide composed of GalA and Gal. Due to the higher yield of DBP1, its structure was studied in greater depth. Methylation experiments indicated that its main chain consisted of 1,4-Galp and 1,4,6-Glcp glycosidic bonds with branch points, consistent with nuclear magnetic resonance (NMR) results. Scanning electron microscopy revealed that DBP1 and DBP2 had distinct filamentous structures yet similar spherical morphologies. Fourier transform infrared spectroscopy and thermal analysis were used to study their functional groups and thermal stability. In activity tests, DBP1 and DBP2 both showed antioxidant activity and could inhibit α-glucosidase and α-amylase, demonstrating hypoglycemic activity.

Conclusion: In conclusion, DBP1 and DBP2, two non-starch polysaccharides, showed good antioxidant and hypoglycemic effects. They can be used as raw materials for functional foods and drugs and merit further development. Maximizing bulbil utilization promotes green, sustainable agriculture and prevents resource waste. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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