用乳胶凝集试验快速检测火鸡鼻气管感染的鸟杆菌

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-04-25 DOI:10.1016/j.psj.2025.105211

Sedigheh Yousefinejad , Mohammad Khosravi , Darioush Gharibi , Mansour Mayahi , Masoud Reza Seyfi Abad Shapouri

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引用次数: 0

摘要

鼻气管鸟杆菌（Ornithobacterium rhinotracheale， ORT）是一种革兰氏阴性病原体，被认为是鸡和火鸡呼吸道疾病的病原体之一。本研究旨在设计并评估胶乳凝集试验检测ORT的诊断有效性。Western blot试验评估了细菌分离株中存在的各种血清型，以及它们的脂多糖的免疫原性。采用不同分离株制备的混合细胞膜抗原设计和优化了ELISA和乳胶凝集试验后，对共有244份有和无呼吸道症状的火鸡血清样本进行了血清学评估。分离的ORT样品的蛋白谱显示分子量为75 kDa和100 kDa的蛋白表达相同。经Western blot分析，所收集的血清显示出与48 kDa、75 kDa和100 kDa抗原反应的共同抗体。胶乳凝集试验与ELISA的敏感性、特异性和相似度分别为87.94、90.29和88.93%。所建立的乳胶凝集试验在最低抗体浓度为308.63 μg/mL时呈阳性反应。结果表明，胶乳凝集试验对火鸡鸟thobacterium感染的快速诊断和控制是有效的。

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Rapid detection of Ornithobacterium rhinotracheale infection by a latex agglutination test in turkeys

Ornithobacterium rhinotracheale (ORT) is a gram-negative pathogen recognized as one of the causative agents of respiratory diseases in chickens and turkeys. This study aimed to design and evaluate the diagnostic validity of the latex agglutination test for ORT detection. A Western blot test was conducted to assess the various serotypes present in the bacterial isolates, as well as the immunogenicity of their lipopolysaccharide. Following the design and optimization of the ELISA and latex agglutination tests using prepared mixed cell membrane antigens from the different isolates, a serological evaluation of the infection was performed on a total of 244 turkey serum samples, both with and without respiratory symptoms.

The protein profiles of the isolated ORT samples revealed equal expression of proteins with molecular weights of 75 kDa and 100 kDa. The collected sera exhibited common antibodies that reacted with antigens weighing 48 kDa, 75 kDa and 100 kDa in Western blot analysis. The sensitivity, specificity and similarity of the latex agglutination test, in comparison to ELISA, were 87.94, 90.29 and 88.93 %, respectively. The developed latex agglutination test resulted in a positive reaction at the presence of a minimum antibody concentration equal to 308.63 μg/mL. The results indicate that the latex agglutination test is effective for the rapid diagnosis and control of Ornithobacterium infection in turkeys.

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.