杀菌剂苯虫啶对稻瘟病菌的抑菌活性调查及抗性风险评价

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-29 DOI:10.1021/acs.jafc.5c02583

Yang Sun, Xinyan Liu, Jinfeng Ying, Ziyun He, Guoqi Tian, Weiye Liu, Xing Chen, Chunyan Gu, Yu Chen

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引用次数: 0

摘要

稻瘟病是稻瘟菌引起的一种破坏性病害，严重威胁着全球粮食生产。本研究旨在研究苯虫脒对稻瘟病菌的抗真菌活性，并评估其耐药风险。苯虫脒对102株稻瘟病菌的平均EC50值为0.0395 μg/mL。苯并虫氟吡喃处理后菌丝和分生孢子出现异常。在相同浓度下，苯并虫氟吡喃的有效性明显高于常用的杀菌剂吡氟甲醚、非诺昔尼和异虫康唑。通过杀菌剂生成，获得了11个抗苯虫吡虫啉突变体，抗性频率为9 × 10-4。此外，这些突变体表现出适应性损失，并与其他琥珀酸脱氢酶抑制剂杀菌剂（如氟沙吡沙和彭氟芬）产生交叉抗性。在苯并虫抗虫年突变体中发现了podhb基因的H245Y/D/L和N203H点突变，podhc基因的S77N点突变，podhd基因的D122N/G点突变，并通过分子对接进行了证实。这些结果表明，苯并虫氟吡喃在稻瘟病菌中具有中等耐药风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Investigation of Antifungal Activity and Assessment of Resistance Risk of Fungicide Benzovindiflupyr in Pyricularia oryzae

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Investigation of Antifungal Activity and Assessment of Resistance Risk of Fungicide Benzovindiflupyr in Pyricularia oryzae

Rice blast is a destructive disease caused by Pyricularia oryzae, which threatens global staple food production. This study aims to investigate antifungal activity and assess the resistance risk of benzovindiflupyr in P. oryzae. The mean EC₅₀ value of benzovindiflupyr against 102 P. oryzae strains was 0.0395 μg/mL. After benzovindiflupyr treatment, mycelia and conidia became abnormal. The effectiveness of benzovindiflupyr was markedly greater compared to the commonly used fungicides pydiflumetofen, fenoxanil, and difenoconazole at the same concentration. Eleven benzovindiflupyr-resistant mutants were obtained with a resistance frequency of 9 × 10^–4 through fungicide generation. In addition, these mutants showed a fitness penalty and cross-resistance with other succinate dehydrogenase inhibitor fungicides, such as fluxapyroxad and penflufen. Point mutations H245Y/D/L and N203H in PoSdhB, S77N in PoSdhC, and D122N/G in PoSdhD were identified in benzovindiflupyr-resistant mutants, which was confirmed by molecular docking. These results suggested a moderate resistance risk associated with benzovindiflupyr in P. oryzae.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.