薏苡仁油生物合成分析有助于溶血磷脂酸酰基转移酶的鉴定

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI:10.1021/acs.jafc.4c12507

Xiaoyan Wei, Jian Wang, Yuping Tan, Haxiu Zhu, Yan Wang, Shufeng Zhou, Juan Guo, Yanan Wang, Luqi Huang

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引用次数: 0

摘要

薏苡仁油（Coix seed oil， CSO）是一种具有显著抗癌潜力的天然物质。然而，薏苡仁脂质生物合成的分子机制和基因调控网络尚不清楚。本文对两个不同脂质含量的薏苡米品种进行了全面的转录组分析。结果显示，通过从头组装共产生了48,110个基因，其中84.45%的基因被数据库成功注释。基于功能注释和基因表达，成功建立了薏苡籽油脂积累和脂肪酸合成关键基因的代谢网络，并通过酵母的异源表达证实了三酰基甘油（TAG）生物合成中的溶血磷脂酸酰基转移酶（LPAT）参与了脂肪酸含量的变化，对不饱和脂肪酸（UFAs）具有显著的选择性结合能力。我们的数据为后续基因功能表征和生物合成途径优化提供了有价值的参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Analysis of Coix Seed Oil Biosynthesis Facilitates the Identification of Lysophosphatidic Acid Acyltransferase

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Analysis of Coix Seed Oil Biosynthesis Facilitates the Identification of Lysophosphatidic Acid Acyltransferase

Coix seed oil (CSO) is a natural substance with significant anticancer potential. However, the molecular mechanism and the gene regulatory network of lipid biosynthesis were not identified in Coix seed. Here, a comprehensive transcriptome analysis was conducted on two Coix varieties with different lipid contents. The results revealed that a total of 48,110 genes were generated by de novo assembly, of which 84.45% genes were successfully annotated by the database. Based on functional annotation and gene expression, the metabolic network of crucial genes for oil accumulation and fatty acid (FA) synthesis in Coix seed has been successfully established, and it has been confirmed that lysophosphatidic acid acyltransferase (LPAT) in triacylglycerol (TAG) biosynthesis contributed to the changes in the content of FAs and had a significant selective binding ability to unsaturated fatty acids (UFAs) by the heterologous expression of yeast. Our data provides valuable references for subsequent gene function characterization and biosynthesis pathway optimization.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.