抗菌和伤口愈合作用与细胞相容性的关系以及与选定的葡萄牙单花蜂蜜的植物化学特征的关系

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2025-04-18 DOI:10.1016/j.jfca.2025.107659

Alexandra M. Machado , Joana Marto , Lídia Maria Gonçalves , Helena Margarida Ribeiro , Aida Duarte , Andreia Tomás , Soraia I. Falcão , Miguel Vilas-Boas , Maria Graça Miguel , Ana Cristina Figueiredo

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引用次数: 0

摘要

由于其在食品和制药工业中的应用，蜂蜜被认为是一种功能性产品。本研究评价了32种葡萄牙单花蜂蜜，并将其活性与麦卢卡蜂蜜850 + 进行了比较。对革兰氏阳性菌、阴性菌和酵母菌进行抑菌活性测定。研究了人角化细胞（HaCaT）的抗氧化活性、伤口愈合能力和细胞活力。采用UPLC/DAD/ESI-MSn进行植物化学谱分析。栗子、铃石楠、桉树、麦卢卡树和草莓树蜂蜜显示出较高的抗氧化活性，对金黄色葡萄球菌有效。香和橙蜂蜜的伤口愈合率分别为89 %和86 %。所有蜂蜜的细胞活力均在76%以上 %。铃石楠蜜和草莓树蜜的总酚含量最高，香蜜和橙蜜的总酚含量较低。植物化学特征分析表明，其主要有两类，一类以类异戊二烯、去异戊二烯、酚酸及其衍生物为主，另一类以黄酮类化合物为主。混合不同的葡萄牙单花蜂蜜可能是一种替代更快的伤口愈合，通过结合他们的多种生物活性成分。收集到的知识可以在配方中进一步探索，考虑到每种蜂蜜的生物活性能力和组成。

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Antimicrobial and wound healing effects associated to cytocompatibility and the relationship with phytochemical profile of selected Portuguese monofloral honeys

Owing to its applications in both food and pharmaceutical industries honey is regarded as a functional product. This study evaluated 32 Portuguese monofloral honeys and their activity was compared with manuka honey 850 + .

The antimicrobial activity was determined against Gram-positive and negative bacteria, and yeast. Antioxidant activity, wound healing ability, and cell viability were studied in the human keratinocyte cell line (HaCaT). Phytochemical profile analysis was performed by UPLC/DAD/ESI-MSⁿ.

Chestnut, bell heather, eucalyptus, manuka, and strawberry tree honeys demonstrated higher antioxidant activity and were effective against S. aureus. Incense and orange honeys showed wound healing rates of 89 % and 86 %, respectively. All the honeys had cell viability above 76 %. Bell heather and strawberry tree honeys showed the highest total phenolic content, while incense and orange honeys, showed the lower ones. Phytochemical profile analysis evidenced two main clusters, one dominated by isoprenoids and norisoprenoids, as well as phenolic acids and derivatives, while the other included flavonoids as main compounds.

Blending different Portuguese monofloral honeys may be an alternative to faster wound healing, by combining their diverse bioactive components. The gathered knowledge can be further explored in formulations that consider the biological activity capacity and composition of each honey.

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.