纳米酶介导的Raman-NLISA双峰免疫传感器用于微囊藻毒素lr的准确灵敏检测

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-04-26 DOI:10.1016/j.foodchem.2025.144480

Wei Zeng , Mingrui Lu , Long Wu , Xin Gao , Qi Chen , Sihang Zhang

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引用次数: 0

摘要

采用介孔SiO2/Au-Pt纳米酶（m-SAP）和纳米小体（A2.3-SBP）构建了拉曼散射和纳米酶联免疫吸附试验（NLISA）双模免疫传感器。氧化TMB在竞争性结合实验中作为拉曼和ELISA信号。在优化条件下，微囊藻素- lr （mcr - lr）浓度与信号呈负相关，在拉曼和ELISA范围内分别为0.1 ~ 100 μg L−1和1.0 ~ 500 μg L−1，检出限（lod, 3σ/S）分别为0.015 μg L−1和0.12 μg L−1。与传统ELISA相比（t-ELISA， LOD为1.36 μg L−1），检测灵敏度提高了90倍以上，11倍以上。该免疫传感器在实际样品中具有优异的准确性，可在45 min内集成在一起进行MC-LR的检测，大大缩短了t-ELISA的检测时间（>2 h）。这种方法显示了通过简单地改变纳米体来检测其他毒素的潜力。

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Nanozyme mediated Raman-NLISA dual-modal immunosensor for accurate and sensitive detection of microcystin-LR

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Nanozyme mediated Raman-NLISA dual-modal immunosensor for accurate and sensitive detection of microcystin-LR

A Raman scattering and nanozyme-linked immunosorbent assay (NLISA) dual modal immunosensor, was constructed by mesoporous SiO₂/Au-Pt nanozymes (m-SAP) and nanobodies (A2.3-SBP). Oxidized TMB served as Raman and ELISA signals in a competitive binding assay. Under optimized conditions, an inverse correlation was established between the Microcystin-LR (MC-LR) concentration and the signals, spanning Raman and ELISA ranges of 0.1–100 μg L⁻¹ and 1.0–500 μg L⁻¹, with limit of detections (LODs, 3σ/S) of 0.015 μg L⁻¹ and 0.12 μg L⁻¹, respectively. The LODs showed over 90 times and 11 times higher sensitivity than that of traditional ELISA (t-ELISA, LOD, 1.36 μg L⁻¹). The immunosensor exhibited excellent accuracy in practical samples, can be integrated together for the detection of MC-LR within 45 min, which greatly short the detection time of t-ELISA (>2 h). This method displayed potential for detecting other toxins by simply changing the nanobodies.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.