硫化氢反应性MRI探针在小鼠结肠癌成像中的应用

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-04-25 DOI:10.1021/acs.analchem.4c07077

Yue Sun, Xingyue Fan, Huiyi Liu, Cheng Zhang, Xianzheng Tan, Guosheng Song

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引用次数: 0

摘要

硫化氢（H2S）是一种重要的气态信号分子，在结肠癌中高度表达。然而，实现对结肠癌深部组织中 H2S 的高灵敏度和特异性成像仍是一项重要挑战。为了克服这一限制，我们开发了一种具有高灵敏度和特异性的 H2S 响应磁探针（HRMP）。HRMP 是用超顺磁性氧化铁和锰卟啉合成的，表面涂有硫化氢响应聚合物。与 H2S 反应后，释放出的纳米粒子聚集在一起，通过偶极效应产生增强的横向弛豫度 (r2)。加入正交叠氮基团可确保 HRMP 对 H2S 产生特异性反应，在 2 小时内迅速发生反应，从而引起 T2 弛豫时间的变化。此外，通过精确调节锰卟啉与氧化铁的喂入比例，HRMP 还具有高灵敏度，检测限低至 8.7 μM。在对 HCT116 结肠癌（H2S 过度表达）的研究中，HRMP 在肿瘤部位产生了明显的负对比。HRMP 显示了结肠癌体内成像的潜力，为肿瘤的早期诊断提供了希望。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Hydrogen Sulfide-Responsive MRI Probe for Imaging Colon Cancer in Mice

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Hydrogen Sulfide-Responsive MRI Probe for Imaging Colon Cancer in Mice

Hydrogen sulfide (H₂S), a significant gaseous signaling molecule, is highly expressed in colon cancer. However, realizing highly sensitive and specific imaging of H₂S in deep colon cancer tissues remains an important challenge. In order to overcome this limitation, we have developed a H₂S-responsive magnetic probe (HRMP) with a high sensitivity and specificity. HRMP is synthesized using superparamagnetic iron oxide and Mn-porphyrin, coated with a hydrogen sulfide-responsive polymer. Upon reaction with H₂S, the released nanoparticles aggregate, producing an enhanced transverse relaxivity (r₂) through the dipolar effect. Incorporation of an ortho azide group ensures that HRMP specifically responds to H₂S, reacting swiftly within 2 h to induce a change in T₂ relaxation time. Additionally, by precisely tuning the feeding ratio of Mn-porphyrin to iron oxide, HRMP was endowed with high sensitivity, achieving a detection limit as low as 8.7 μM. In studies with HCT116 colon cancer, where H₂S is overexpressed, HRMP generated a distinct negative contrast at the tumor site. HRMP shows potential for in vivo imaging of colon cancer, offering promise for the early diagnosis of tumors.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.