Keshab Bhattarai, Thomas Majer, Manuela Haussmann, Dieter Schollmeyer, Markus Kramer, Feyisara Eyiwumi Oni, Monica Höfte, Rabea Voget, Michael Gütschow, Natalia Ruetalo, Michael Schindler, Jan Straetener, Tatjana Wannenwetsch, Heike Brötz-Oesterhelt, Ryan Karongo, Benedikt Masberg, Michael Lämmerhofer, Rosanna Catherine Hennessy, Carly R. Muletz-Wolz and Harald Gross*,
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Salamandamide Lipodipeptides Are Biosynthetic Intermediate Shunt Products of the Nonamodular Nonribosomal Peptide Assembly Lines of the Viscosin Family
Chemical investigation of a salamander-mucus-associated Pseudomonas tolaasii strain led to the isolation and chemical characterization of salamandamide A, a new lipo-dipeptide, along with known lipopeptides of the pseudodesmin and tolaasin class. Genome mining revealed that no specific gene cluster codes for the biosynthesis of salamandamide A. Stereochemical analyses and mutagenesis experiments linked the biosynthesis of the lipo-dipeptide salamandamide A to the NRPS gene cluster of the lipo-nonapeptide pseudodesmin. Further chemical investigations showed that this finding appears to be a broader concept and that all nonamodular NRPS gene clusters of the viscosin family were capable to produce, beside the expected lipo-nonapeptide, the corresponding lipo-dipeptide as a shunt product which also led to the discovery of salamandamide B from Pseudomonas lactis SS101.
期刊介绍：
The Journal of Natural Products invites and publishes papers that make substantial and scholarly contributions to the area of natural products research. Contributions may relate to the chemistry and/or biochemistry of naturally occurring compounds or the biology of living systems from which they are obtained.
Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.
When new compounds are reported, manuscripts describing their biological activity are much preferred.
Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.
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