Tábata A. Bublitz, Heberto Rodas-Gaitan, Rainer Georg Joergensen, Vincent Masson, Juergen Fritz
{"title":"有机与生物动力土地管理下葡萄园土壤微生物功能多样性指标研究","authors":"Tábata A. Bublitz, Heberto Rodas-Gaitan, Rainer Georg Joergensen, Vincent Masson, Juergen Fritz","doi":"10.1186/s40538-025-00766-2","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>An on-vineyard approach was used to investigate the effects of biodynamic (BD) preparations on microbial functional diversity and extracellular polymeric substances (EPS) in four vineyards on different bedrocks under organic management.</p><h3>Methods</h3><p>Soil organic carbon (SOC), total N, microbial biomass carbon (MBC), multi-substrate-induced respiration (MSIR), with 17 substrates and H<sub>2</sub>O, EPS, and glomalin-related soil protein (GRSP) were measured in soils taken from vineyards without (BD -) and with (BD +) biodynamic preparations.</p><h3>Results</h3><p>All parameters showed a significant vineyard effect due to specific soil types, and all, except GRSP and the GRSP/EPS-prot, showed an effect of BD preparations. BD + resulted in significant increases in MBC, MBC/SOC, EPS-carbohydrates and respiratory response for most vineyards but a significant decrease in the <i>q</i>CO<sub>2</sub> values. The significant increases in mean SOC, Total N, CO<sub>2</sub>C, and the ratios EPS-carb/EPS-prot with BD +, as well as decreases in mean EPS-proteins and the ratios EPS-carb/MBC and EPS-prot/MBC were not reflected by significant differences between the individual vineyards. GRSP was negatively related to the microbial respiratory response of all substrates added.</p><h3>Conclusions</h3><p>The significant effects of vineyard are closely connected to soil pH and texture. The significant increases in BD +, visible in MBC, MBC/SOC and EPS-carbohydrates, and the latter positive correlation with the respiratory response demonstrates an improvement of soil chemical and biological properties for most vineyards. Lower ratios of EPS-carb/MBC, EPS-prot/MBC and amounts of EPS-protein indicate that soil microorganisms diverted less substrate to the formation of EPS and more to the production of microbial biomass. GRSP and GRSP/EPS-prot did not show any explainable pattern. Future investigations on soil structure might be of benefit for studies on the effect of BD preparations in vineyards.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00766-2","citationCount":"0","resultStr":"{\"title\":\"Microbial functional diversity indicators in vineyard soils under organic and biodynamic land management\",\"authors\":\"Tábata A. 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BD + resulted in significant increases in MBC, MBC/SOC, EPS-carbohydrates and respiratory response for most vineyards but a significant decrease in the <i>q</i>CO<sub>2</sub> values. The significant increases in mean SOC, Total N, CO<sub>2</sub>C, and the ratios EPS-carb/EPS-prot with BD +, as well as decreases in mean EPS-proteins and the ratios EPS-carb/MBC and EPS-prot/MBC were not reflected by significant differences between the individual vineyards. GRSP was negatively related to the microbial respiratory response of all substrates added.</p><h3>Conclusions</h3><p>The significant effects of vineyard are closely connected to soil pH and texture. The significant increases in BD +, visible in MBC, MBC/SOC and EPS-carbohydrates, and the latter positive correlation with the respiratory response demonstrates an improvement of soil chemical and biological properties for most vineyards. 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Microbial functional diversity indicators in vineyard soils under organic and biodynamic land management
Background
An on-vineyard approach was used to investigate the effects of biodynamic (BD) preparations on microbial functional diversity and extracellular polymeric substances (EPS) in four vineyards on different bedrocks under organic management.
Methods
Soil organic carbon (SOC), total N, microbial biomass carbon (MBC), multi-substrate-induced respiration (MSIR), with 17 substrates and H2O, EPS, and glomalin-related soil protein (GRSP) were measured in soils taken from vineyards without (BD -) and with (BD +) biodynamic preparations.
Results
All parameters showed a significant vineyard effect due to specific soil types, and all, except GRSP and the GRSP/EPS-prot, showed an effect of BD preparations. BD + resulted in significant increases in MBC, MBC/SOC, EPS-carbohydrates and respiratory response for most vineyards but a significant decrease in the qCO2 values. The significant increases in mean SOC, Total N, CO2C, and the ratios EPS-carb/EPS-prot with BD +, as well as decreases in mean EPS-proteins and the ratios EPS-carb/MBC and EPS-prot/MBC were not reflected by significant differences between the individual vineyards. GRSP was negatively related to the microbial respiratory response of all substrates added.
Conclusions
The significant effects of vineyard are closely connected to soil pH and texture. The significant increases in BD +, visible in MBC, MBC/SOC and EPS-carbohydrates, and the latter positive correlation with the respiratory response demonstrates an improvement of soil chemical and biological properties for most vineyards. Lower ratios of EPS-carb/MBC, EPS-prot/MBC and amounts of EPS-protein indicate that soil microorganisms diverted less substrate to the formation of EPS and more to the production of microbial biomass. GRSP and GRSP/EPS-prot did not show any explainable pattern. Future investigations on soil structure might be of benefit for studies on the effect of BD preparations in vineyards.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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