Enhancing bioavailability and functionality of plant peptides and proteins: A review of novel strategies for food and pharmaceutical applications

Plant-derived peptides and proteins are emerging as versatile bioactive ingredients in functional food and pharmaceutical sectors due to their diverse health benefits. However, their practical applications are often limited by poor bioavailability and functional instability. This review evaluates key determinants of plant peptide/protein bioactivity, including physicochemical properties, anti-nutritional components, food matrix interactions, and gastrointestinal digestion conditions. Strategies to enhance their functionality and bioavailability are systematically discussed, focusing on absorption enhancers, structural modifications, protease inhibitors, and colloidal delivery systems (e.g., liposomes, emulsions, nanoparticles). Recent advancements highlight targeted enzymatic hydrolysis and fermentation as effective methods to generate bioactive peptides with improved therapeutic properties. Additionally, physical/chemical modifications enhance stability against proteolysis and improve functional performance. Innovations in plant-derived protein-based delivery systems, such as nanoparticles, demonstrate promise in protecting bioactive compounds and optimizing bioavailability. Collectively, these approaches provide a roadmap for developing next-generation plant-protein products, addressing challenges in bioactivity retention and gastrointestinal absorption.