{"title":"基于新筛选靶点的新型proteus特异性压电传感器","authors":"Hui Yu, Jinxia Ma, Yusheng Liao, Jiali Ren* and Fengjiao He*, ","doi":"10.1021/acs.analchem.4c0700410.1021/acs.analchem.4c07004","DOIUrl":null,"url":null,"abstract":"<p ><i>Proteus</i> spp. are conditionally pathogenic bacteria that cause urinary tract infections, gastrointestinal diseases, and food poisoning. Accurate and rapid detection of <i>Proteus</i> is crucial in medical diagnosis and food safety. In the present study, a new 16S rRNA sequence fragment was identified as a <i>Proteus</i> detection target. A novel <i>Proteus</i> piezoelectric sensor was constructed based on this screened target by combining catalytic hairpin self-assembly target cycle technology, hybrid chain reaction amplification technology, and a piezoelectric sensor. The proposed method was rapid and highly specific; 10–10<sup>6</sup> CFU/mL <i>Proteus</i> could be detected within 3 h in the presence of <i>Escherichia coli, Staphylococcus aureus</i>, and other common bacteria. The limit of detection was as low as 10 CFU/mL. The proposed method could offer a rapid, sensitive, and specific approach for <i>Proteus</i> detection with broad application potential.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 15","pages":"8378–8384 8378–8384"},"PeriodicalIF":6.7000,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Novel Proteus-Specific, Piezoelectric Sensor Based on Newly Screened Targets\",\"authors\":\"Hui Yu, Jinxia Ma, Yusheng Liao, Jiali Ren* and Fengjiao He*, \",\"doi\":\"10.1021/acs.analchem.4c0700410.1021/acs.analchem.4c07004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p ><i>Proteus</i> spp. are conditionally pathogenic bacteria that cause urinary tract infections, gastrointestinal diseases, and food poisoning. Accurate and rapid detection of <i>Proteus</i> is crucial in medical diagnosis and food safety. In the present study, a new 16S rRNA sequence fragment was identified as a <i>Proteus</i> detection target. A novel <i>Proteus</i> piezoelectric sensor was constructed based on this screened target by combining catalytic hairpin self-assembly target cycle technology, hybrid chain reaction amplification technology, and a piezoelectric sensor. The proposed method was rapid and highly specific; 10–10<sup>6</sup> CFU/mL <i>Proteus</i> could be detected within 3 h in the presence of <i>Escherichia coli, Staphylococcus aureus</i>, and other common bacteria. The limit of detection was as low as 10 CFU/mL. The proposed method could offer a rapid, sensitive, and specific approach for <i>Proteus</i> detection with broad application potential.</p>\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"97 15\",\"pages\":\"8378–8384 8378–8384\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2025-04-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.analchem.4c07004\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.analchem.4c07004","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A Novel Proteus-Specific, Piezoelectric Sensor Based on Newly Screened Targets
Proteus spp. are conditionally pathogenic bacteria that cause urinary tract infections, gastrointestinal diseases, and food poisoning. Accurate and rapid detection of Proteus is crucial in medical diagnosis and food safety. In the present study, a new 16S rRNA sequence fragment was identified as a Proteus detection target. A novel Proteus piezoelectric sensor was constructed based on this screened target by combining catalytic hairpin self-assembly target cycle technology, hybrid chain reaction amplification technology, and a piezoelectric sensor. The proposed method was rapid and highly specific; 10–106 CFU/mL Proteus could be detected within 3 h in the presence of Escherichia coli, Staphylococcus aureus, and other common bacteria. The limit of detection was as low as 10 CFU/mL. The proposed method could offer a rapid, sensitive, and specific approach for Proteus detection with broad application potential.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.