基于新筛选靶点的新型proteus特异性压电传感器

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Hui Yu, Jinxia Ma, Yusheng Liao, Jiali Ren* and Fengjiao He*, 
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引用次数: 0

摘要

变形杆菌属有条件致病菌,可引起尿路感染、胃肠道疾病和食物中毒。准确、快速检测变形杆菌对医疗诊断和食品安全至关重要。本研究鉴定了一个新的16S rRNA序列片段作为Proteus的检测靶点。结合催化发夹自组装靶标循环技术、杂化链反应放大技术和压电传感器,构建了一种新型的Proteus压电传感器。该方法快速、特异度高;在大肠杆菌、金黄色葡萄球菌等常见细菌存在的情况下,3 h内可检出10 ~ 106 CFU/mL的Proteus。检出限低至10 CFU/mL。该方法为Proteus的检测提供了一种快速、灵敏、特异的方法,具有广阔的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A Novel Proteus-Specific, Piezoelectric Sensor Based on Newly Screened Targets

A Novel Proteus-Specific, Piezoelectric Sensor Based on Newly Screened Targets

Proteus spp. are conditionally pathogenic bacteria that cause urinary tract infections, gastrointestinal diseases, and food poisoning. Accurate and rapid detection of Proteus is crucial in medical diagnosis and food safety. In the present study, a new 16S rRNA sequence fragment was identified as a Proteus detection target. A novel Proteus piezoelectric sensor was constructed based on this screened target by combining catalytic hairpin self-assembly target cycle technology, hybrid chain reaction amplification technology, and a piezoelectric sensor. The proposed method was rapid and highly specific; 10–106 CFU/mL Proteus could be detected within 3 h in the presence of Escherichia coli, Staphylococcus aureus, and other common bacteria. The limit of detection was as low as 10 CFU/mL. The proposed method could offer a rapid, sensitive, and specific approach for Proteus detection with broad application potential.

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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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