远红外线对CRS诱导小鼠抑郁样行为的影响及机制

IF 3.7 3区医学 Q2 NEUROSCIENCES

Brain Research Bulletin Pub Date : 2025-04-17 DOI:10.1016/j.brainresbull.2025.111348

Yian Ling , Yejun Gao , Wanbin Liu , Jing Li , Lijuan Nie , Cuizhen Zhu , Qingrong Xia

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引用次数: 0

摘要

目的：远红外线（FIR）是一种电磁波，已知对各种病理生理状况（包括糖尿病、心血管疾病、压力和抑郁等）具有健康益处。然而，FIR对重度抑郁症（MDD）的确切影响及其潜在的分子机制尚不清楚。在此，我们旨在研究FIR对小鼠抑郁样行为的影响并阐明其分子机制。方法采用慢性抑制应激法（CRS）建立小鼠抑郁模型。进行行为测试以评估抑郁样行为的改变。采用生化方法测定小鼠血清中IL-1β、IL-6、TNF-α、S100β、IL-17、褪黑素（MT）、5-羟色胺（5-HT）、谷胱甘肽（GSH）、丙二醛（MDA）、脑源性神经营养因子（BDNF）、皮质酮（CORT）水平。同样，采用生化方法测定小鼠脑内IL-1β、IL-6、TNF-α、S100β、IL-17和MT的水平。采用苏木精-伊红染色和尼氏染色检测小鼠海马的形态学变化。透射电镜观察海马神经元和小胶质细胞的结构和线粒体形态。结果行为测试结果显示，FIR可减轻CRS诱导的抑郁样行为。FIR还逆转了外周和大脑中IL-1β、IL-6、TNF-α和相关细胞因子的水平。苏木精-伊红染色和尼氏染色结果显示，FIR对小鼠海马损伤有改善作用。此外，透射电镜显示，FIR减轻了CRS对海马神经元和小胶质细胞的损伤。结论FIR可改善CRS诱导的小鼠抑郁样行为。FIR可逆转外周和脑内相关细胞因子水平，减轻神经元和小胶质细胞损伤，这可能是其潜在的分子机制。

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The effects and mechanisms of far-infrared ray on depression-like behavior induced by CRS in mice

Purpose

Far-infrared ray (FIR) is an electromagnetic wave known to impart health benefits against various pathophysiological conditions, including diabetes mellitus, renocardiovascular disorders, stress, and depression, among others. However, the precise impact of FIR on major depressive disorder (MDD) and the underlying molecular mechanisms remain unclear. Here, we aimed to investigate the effects and elucidate the molecular mechanisms of FIR on depression-like behavior in mice.

Methods

A mouse model of depression was established using chronic restraint stress (CRS). Behavioral tests were performed to assess alterations in depression-like behaviors. Biochemical methods were employed to measure the levels of IL-1β, IL-6, TNF-α, S100β, IL-17, melatonin (MT), 5-hydroxytryptamine (5-HT), glutathione (GSH), malondialdehyde (MDA), brain-derived neurotrophic factor (BDNF), and corticosterone (CORT) in mice serum. Similarly, the levels of IL-1β, IL-6, TNF-α, S100β, IL-17, and MT in mice brains were measured using biochemical methods. Hematoxylin-eosin and Nissl staining were utilized to detect morphological changes in the mice hippocampus. In addition, the structure and mitochondrial morphology of hippocampal neurons and microglia were studied using transmission electron microscopy (TEM).

Results

The results of behavioral tests revealed that FIR mitigated the depression-like behaviors induced by CRS. FIR also reversed the levels of IL-1β, IL-6, TNF-α, and related cytokines in the periphery and brain. The results of hematoxylin-eosin and Nissl staining showed that FIR improved the damage of mice's hippocampus. Additionally, TEM revealed that FIR alleviated the damage of CRS to hippocampal neurons and microglia.

Conclusion

Our findings suggest that FIR can ameliorate depression-like behavior induced by CRS in mice. FIR can reverse the levels of related cytokines in the periphery and brain, and alleviate damage to neurons and microglia, which may constitute its underlying molecular mechanism.

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来源期刊

Brain Research Bulletin 医学-神经科学

CiteScore

6.90

自引率

2.60%

发文量

253

审稿时长

67 days

期刊介绍： The Brain Research Bulletin (BRB) aims to publish novel work that advances our knowledge of molecular and cellular mechanisms that underlie neural network properties associated with behavior, cognition and other brain functions during neurodevelopment and in the adult. Although clinical research is out of the Journal''s scope, the BRB also aims to publish translation research that provides insight into biological mechanisms and processes associated with neurodegeneration mechanisms, neurological diseases and neuropsychiatric disorders. The Journal is especially interested in research using novel methodologies, such as optogenetics, multielectrode array recordings and life imaging in wild-type and genetically-modified animal models, with the goal to advance our understanding of how neurons, glia and networks function in vivo.