农产品和土壤中氟沙芬快速检测的半抗原设计、单克隆抗体制备及免疫分析方法的发展

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-04-13 DOI:10.1016/j.foodchem.2025.144305

Qingqing Gao , Jingqian Huo , Chang Wang , Jiayu Cao , Haijiao Dong , Jia Kang , Kaiwen Li , Zexiu An , Lai Chen , Jinlin Zhang

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引用次数: 0

摘要

氟草胺是一种二苯醚除草剂，其误用在土壤或农产品中的残留不仅影响敏感作物的产量和质量，而且通过食物链对人类健康构成威胁。为了实现快速、灵敏的原位检测，本研究在前人工作的基础上重新设计合成了两种新的半抗原，并获得了三种单克隆抗体（mab）。建立了基于异种半抗原的间接竞争ELISA （ic-ELISA）和横向流动免疫分析法（LFIA）检测弓形虫抗原的方法。ic-ELISA的IC50值为1.56 ± 0.24 ng/mL，线性范围为0.08 ~ 28.86 ng/mL。本研究开发的ic-ELISA的灵敏度比我们以往的工作提高了近25倍。LFIA的视觉检出限（vLOD）为2.5 ng/mL，截止值为16.0 ng/mL。本研究对实现农产品、土壤等环境基质中氟沙芬的快速现场检测具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Rational hapten design, monoclonal antibody preparation, and immunoassays development for rapid detection of fomesafen in agricultural products and soil

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Rational hapten design, monoclonal antibody preparation, and immunoassays development for rapid detection of fomesafen in agricultural products and soil

Fomesafen is a diphenyl ether herbicide, and the residues in soil or agricultural products caused by its misused not only affect the yield and quality of sensitive crops, but also pose a threat to human health through the food chain. In order to achieve rapid and sensitive detection of fomesafen in situ, this study redesigned and synthesized two new haptens based on previous work, and obtained three monoclonal antibodies (mAbs). Indirect competitive ELISA (ic-ELISA) and lateral flow immunoassay (LFIA) methods were established based on heterologous haptens for fomesafen detection. The IC₅₀ value and linear range for ic-ELISA were 1.56 ± 0.24 ng/mL and 0.08–28.86 ng/mL, respectively. The sensitivity of ic-ELISA developed in this study increased by nearly 25 times compared to our previous work. Meanwhile, the visual limit of detection (vLOD) of LFIA was 2.5 ng/mL with the cutoff value of 16.0 ng/mL. This study is of great significance for achieving rapid on-site detection of fomesafen in agricultural products, soil, and other environmental matrices.

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.