黄精可溶性多糖(PCHP-1A)的结构表征和生物活性评价:抗氧化和α-葡萄糖苷酶抑制活性

IF 5.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Lunbin Xia, Cunwu Chen, Shaoshuai Bi, Yafei Zhang, Naidong Chen, Jing Feng
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引用次数: 0

摘要

近年来,中草药多糖的生物活性越来越受到人们的关注。黄精是一种应用广泛的中草药,具有重要的保健价值。然而,多糖结构与生物功能之间的构效关系尚未得到充分阐明。方法采用热水提取-醇沉法提取粗黄精多糖。提取液经DEAE sepplife FF层析纯化,得到PCHP-1和PCHP-2两个部分。由于PCHP-1的含量最高且溶解度较好,因此采用sepphacryl S-400HR柱层析进一步纯化PCHP-1,得到纯化后的PCHP-1A。随后,通过单糖组成分析、扫描电镜(SEM)、傅里叶变换红外光谱(FT-IR)、甲基化分析、核磁共振(NMR)等手段对PCHP-1A的结构特征进行了综合分析。此外,通过体外实验对PCHP-1A的抗氧化和降血糖活性进行了评价。结果PCHP-1A平均分子量为2.90 × 104 Da,由果糖(Fru)、葡萄糖(Glc)、半乳糖(Gal)、甘露糖(Man)、阿拉伯糖(Ara)和鼠李糖(Rha)组成。PCHP-1A具有致密卷曲的球形分子构象,SEM分析显示其光滑、多孔、碎屑或片层结构。综合FT-IR、GC-MS和1D/2D NMR分析表明,PCHP-1A的主链由→1)-β-d-Fruf-(2→、→1,6)-β-d-Fruf-(2→和→6)-α-d- glcp组成,支链主要由β-d-Fruf-(2→连接在→1,6)-β-d-Fruf-(2→的O-6位置上的β-d-Fruf-(2→)组成。生物活性实验表明,在浓度为4.0 mg/mL时,PCHP-1A对DPPH的清除率为56.92±1.42%,对·OH自由基的抑制能力为23.27±1.80 U/mL。此外,PCHP-1A具有显著的降糖活性,IC50值为5.15±1.09 mg/mL。结论本研究成功分离纯化了黄精多糖,阐明了其基本结构,并对其抗氧化和降血糖活性进行了研究。研究结果为了解黄精多糖的构效关系提供了理论基础,提示黄精多糖可能是一种潜在的天然抗氧化剂和降糖剂。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structural characterization and bioactivity evaluation of a soluble polysaccharide (PCHP-1A) from Polygonatum cyrtonema Hua: antioxidant and α-glucosidase inhibitory activities

Background

In recent years, the biological activities of polysaccharides derived from Chinese herbal medicones have garnered increasing attention. Polygonatum cyrtonema Hua, a widely used traditional Chinese medicinal herb, possesses significant healthcare value. However, the structure–activity relationship between polysaccharide structures and biological functions remains to be fully elucidated.

Methods

Crude Polygonatum cyrtonema Hua polysaccharides (PCHP) were extracted using hot water extraction followed by alcohol precipitation. The extract was subsequently purified through DEAE seplife FF chromatography, yielding two franctions: PCHP-1 and PCHP-2. Because of the highest content and the better solubility, PCHP-1 was further purified using Sephacryl S-400HR column chromatography to obtain a purified fraction designated as PCHP-1A. Subsequently, The structural characteristics of PCHP-1A were comprehensively analyzed through monosaccharide composition analysis, scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy. Additionally, the antioxidant and hypoglycemic activities of PCHP-1A were evaluated though in vitro experiments.

Results

Structural analysis revealed that PCHP-1A, with an average molecular weight of 2.90 × 104 Da, is comprised of fructose (Fru), glucose (Glc), galactose (Gal), mannose (Man), arabinose (Ara) and rhamnose (Rha). PCHP-1A exhibited a compact and curly spherical molecule conformation, and SEM analysis revealed its smooth, porous, clastic or lamellar microstructure. Comprehensive FT-IR, GC–MS, and 1D/2D NMR analyses indicated that the main chain of PCHP-1A consists of → 1)-β-d-Fruf-(2 → , → 1,6)-β-d-Fruf-(2 → , and → 6)-α-d-Glcp, with branch chains primarily composed of β-d-Fruf-(2 → linked at the O-6 position of → 1,6)-β-d-Fruf-(2 →. Biological activity assays demonstrated that at a concentration of 4.0 mg/mL, PCHP-1A exhibited a DPPH scavenging rate of 56.92 ± 1.42% and an inhibitory capacity against ·OH radicals of 23.27 ± 1.80 U/mL. Furthermore, PCHP-1A showed significant hypoglycemic activity, with an IC50 value of 5.15 ± 1.09 mg/mL.

Conclusions

This study successfully isolated and purified polysaccharide from Polygonatum cyrtonema Hua, elucidated its fundamental structure, and investigated its antioxidant and hypoglycemic activities. The findings provide a theoretical foundation for understanding the structure–activity relationship of the polysaccharide and suggest that Polygonatum cyrtonema Hua polysaccharide may be a natural potential antioxidant and hypoglycemic agent.

Graphical Abstract

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来源期刊
Chemical and Biological Technologies in Agriculture
Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.80
自引率
3.00%
发文量
83
审稿时长
15 weeks
期刊介绍: Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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