{"title":"黄精可溶性多糖（PCHP-1A）的结构表征和生物活性评价：抗氧化和α-葡萄糖苷酶抑制活性","authors":"Lunbin Xia, Cunwu Chen, Shaoshuai Bi, Yafei Zhang, Naidong Chen, Jing Feng","doi":"10.1186/s40538-025-00768-0","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>In recent years, the biological activities of polysaccharides derived from Chinese herbal medicones have garnered increasing attention. <i>Polygonatum cyrtonema</i> Hua, a widely used traditional Chinese medicinal herb, possesses significant healthcare value. However, the structure–activity relationship between polysaccharide structures and biological functions remains to be fully elucidated.</p><h3>Methods</h3><p>Crude <i>Polygonatum cyrtonema</i> Hua polysaccharides (PCHP) were extracted using hot water extraction followed by alcohol precipitation. The extract was subsequently purified through DEAE seplife FF chromatography, yielding two franctions: PCHP-1 and PCHP-2. Because of the highest content and the better solubility, PCHP-1 was further purified using Sephacryl S-400HR column chromatography to obtain a purified fraction designated as PCHP-1A. Subsequently, The structural characteristics of PCHP-1A were comprehensively analyzed through monosaccharide composition analysis, scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy. Additionally, the antioxidant and hypoglycemic activities of PCHP-1A were evaluated though in vitro experiments.</p><h3>Results</h3><p>Structural analysis revealed that PCHP-1A, with an average molecular weight of 2.90 × 10<sup>4</sup> Da, is comprised of fructose (Fru), glucose (Glc), galactose (Gal), mannose (Man), arabinose (Ara) and rhamnose (Rha). PCHP-1A exhibited a compact and curly spherical molecule conformation, and SEM analysis revealed its smooth, porous, clastic or lamellar microstructure. Comprehensive FT-IR, GC–MS, and 1D/2D NMR analyses indicated that the main chain of PCHP-1A consists of → 1)-β-<span>d</span>-Fru<i>f</i>-(2 → , → 1,6)-β-<span>d</span>-Fru<i>f</i>-(2 → , and → 6)-α-<span>d</span>-Glc<i>p,</i> with branch chains primarily composed of β-<span>d</span>-Fru<i>f</i>-(2 → linked at the <i>O</i>-6 position of → 1,6)-β-<span>d</span>-Fru<i>f</i>-(2 →. Biological activity assays demonstrated that at a concentration of 4.0 mg/mL, PCHP-1A exhibited a DPPH scavenging rate of 56.92 ± 1.42% and an inhibitory capacity against <b>·</b>OH radicals of 23.27 ± 1.80 U/mL. Furthermore, PCHP-1A showed significant hypoglycemic activity, with an IC<sub>50</sub> value of 5.15 ± 1.09 mg/mL.</p><h3>Conclusions</h3><p>This study successfully isolated and purified polysaccharide from <i>Polygonatum cyrtonema</i> Hua, elucidated its fundamental structure, and investigated its antioxidant and hypoglycemic activities. The findings provide a theoretical foundation for understanding the structure–activity relationship of the polysaccharide and suggest that <i>Polygonatum cyrtonema</i> Hua polysaccharide may be a natural potential antioxidant and hypoglycemic agent.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00768-0","citationCount":"0","resultStr":"{\"title\":\"Structural characterization and bioactivity evaluation of a soluble polysaccharide (PCHP-1A) from Polygonatum cyrtonema Hua: antioxidant and α-glucosidase inhibitory activities\",\"authors\":\"Lunbin Xia, Cunwu Chen, Shaoshuai Bi, Yafei Zhang, Naidong Chen, Jing Feng\",\"doi\":\"10.1186/s40538-025-00768-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>In recent years, the biological activities of polysaccharides derived from Chinese herbal medicones have garnered increasing attention. <i>Polygonatum cyrtonema</i> Hua, a widely used traditional Chinese medicinal herb, possesses significant healthcare value. However, the structure–activity relationship between polysaccharide structures and biological functions remains to be fully elucidated.</p><h3>Methods</h3><p>Crude <i>Polygonatum cyrtonema</i> Hua polysaccharides (PCHP) were extracted using hot water extraction followed by alcohol precipitation. The extract was subsequently purified through DEAE seplife FF chromatography, yielding two franctions: PCHP-1 and PCHP-2. Because of the highest content and the better solubility, PCHP-1 was further purified using Sephacryl S-400HR column chromatography to obtain a purified fraction designated as PCHP-1A. Subsequently, The structural characteristics of PCHP-1A were comprehensively analyzed through monosaccharide composition analysis, scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy. Additionally, the antioxidant and hypoglycemic activities of PCHP-1A were evaluated though in vitro experiments.</p><h3>Results</h3><p>Structural analysis revealed that PCHP-1A, with an average molecular weight of 2.90 × 10<sup>4</sup> Da, is comprised of fructose (Fru), glucose (Glc), galactose (Gal), mannose (Man), arabinose (Ara) and rhamnose (Rha). PCHP-1A exhibited a compact and curly spherical molecule conformation, and SEM analysis revealed its smooth, porous, clastic or lamellar microstructure. Comprehensive FT-IR, GC–MS, and 1D/2D NMR analyses indicated that the main chain of PCHP-1A consists of → 1)-β-<span>d</span>-Fru<i>f</i>-(2 → , → 1,6)-β-<span>d</span>-Fru<i>f</i>-(2 → , and → 6)-α-<span>d</span>-Glc<i>p,</i> with branch chains primarily composed of β-<span>d</span>-Fru<i>f</i>-(2 → linked at the <i>O</i>-6 position of → 1,6)-β-<span>d</span>-Fru<i>f</i>-(2 →. Biological activity assays demonstrated that at a concentration of 4.0 mg/mL, PCHP-1A exhibited a DPPH scavenging rate of 56.92 ± 1.42% and an inhibitory capacity against <b>·</b>OH radicals of 23.27 ± 1.80 U/mL. Furthermore, PCHP-1A showed significant hypoglycemic activity, with an IC<sub>50</sub> value of 5.15 ± 1.09 mg/mL.</p><h3>Conclusions</h3><p>This study successfully isolated and purified polysaccharide from <i>Polygonatum cyrtonema</i> Hua, elucidated its fundamental structure, and investigated its antioxidant and hypoglycemic activities. The findings provide a theoretical foundation for understanding the structure–activity relationship of the polysaccharide and suggest that <i>Polygonatum cyrtonema</i> Hua polysaccharide may be a natural potential antioxidant and hypoglycemic agent.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":512,\"journal\":{\"name\":\"Chemical and Biological Technologies in Agriculture\",\"volume\":\"12 1\",\"pages\":\"\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2025-04-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00768-0\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical and Biological Technologies in Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40538-025-00768-0\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-025-00768-0","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Structural characterization and bioactivity evaluation of a soluble polysaccharide (PCHP-1A) from Polygonatum cyrtonema Hua: antioxidant and α-glucosidase inhibitory activities
Background
In recent years, the biological activities of polysaccharides derived from Chinese herbal medicones have garnered increasing attention. Polygonatum cyrtonema Hua, a widely used traditional Chinese medicinal herb, possesses significant healthcare value. However, the structure–activity relationship between polysaccharide structures and biological functions remains to be fully elucidated.
Methods
Crude Polygonatum cyrtonema Hua polysaccharides (PCHP) were extracted using hot water extraction followed by alcohol precipitation. The extract was subsequently purified through DEAE seplife FF chromatography, yielding two franctions: PCHP-1 and PCHP-2. Because of the highest content and the better solubility, PCHP-1 was further purified using Sephacryl S-400HR column chromatography to obtain a purified fraction designated as PCHP-1A. Subsequently, The structural characteristics of PCHP-1A were comprehensively analyzed through monosaccharide composition analysis, scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy. Additionally, the antioxidant and hypoglycemic activities of PCHP-1A were evaluated though in vitro experiments.
Results
Structural analysis revealed that PCHP-1A, with an average molecular weight of 2.90 × 104 Da, is comprised of fructose (Fru), glucose (Glc), galactose (Gal), mannose (Man), arabinose (Ara) and rhamnose (Rha). PCHP-1A exhibited a compact and curly spherical molecule conformation, and SEM analysis revealed its smooth, porous, clastic or lamellar microstructure. Comprehensive FT-IR, GC–MS, and 1D/2D NMR analyses indicated that the main chain of PCHP-1A consists of → 1)-β-d-Fruf-(2 → , → 1,6)-β-d-Fruf-(2 → , and → 6)-α-d-Glcp, with branch chains primarily composed of β-d-Fruf-(2 → linked at the O-6 position of → 1,6)-β-d-Fruf-(2 →. Biological activity assays demonstrated that at a concentration of 4.0 mg/mL, PCHP-1A exhibited a DPPH scavenging rate of 56.92 ± 1.42% and an inhibitory capacity against ·OH radicals of 23.27 ± 1.80 U/mL. Furthermore, PCHP-1A showed significant hypoglycemic activity, with an IC50 value of 5.15 ± 1.09 mg/mL.
Conclusions
This study successfully isolated and purified polysaccharide from Polygonatum cyrtonema Hua, elucidated its fundamental structure, and investigated its antioxidant and hypoglycemic activities. The findings provide a theoretical foundation for understanding the structure–activity relationship of the polysaccharide and suggest that Polygonatum cyrtonema Hua polysaccharide may be a natural potential antioxidant and hypoglycemic agent.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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