迷迭香的代谢谱分析及对淡紫色扁球菌的杀虫活性研究

IF 5.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Urvashi Kashyap, S. G. Eswara Reddy
{"title":"迷迭香的代谢谱分析及对淡紫色扁球菌的杀虫活性研究","authors":"Urvashi Kashyap,&nbsp;S. G. Eswara Reddy","doi":"10.1186/s40538-025-00761-7","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Sucking insects are major threat to agricultural and horticultural crops. Indiscriminate application of chemical insecticides for the control of pests leads to the development of resistance, harmful to non-target organisms, consumers’ health, the environment, etc. Therefore, botanical insecticides are alternate to synthetic pesticides for the control of sucking pests. In the present investigation, chemical constituents, metabolic profile, and insecticidal activities of <i>Rosmarinus officinalis</i> L. (Lamiaceae) ethanolic aqueous extract (EAE), fractions and compounds were screened against <i>Aphis craccivora</i> Koch (Hemiptera: Aphididae) and <i>Planococcus lilacinus</i> Cockerell (Hemiptera: Pseudococcidae)<i>.</i></p><h3>Results</h3><p>Gas chromatography (GC) and gas chromatography–mass spectrometry (GC–MS) analysis showed that linolenic acid (24.97%), 1,8-cineole (14.26%), myrcene (10.67%), hexadecenoic acid (9.91%), and camphene (7.12%) were the major constituents in the <i>n</i>-hexane fraction. UHPLC–ESI-QTOF-IMS analysis of ethanolic aqueous extract (EAE) showed the presence of palmitoleic acid, 4-ethoxy ethyl benzoate, 7-methylrosmanol, and diosmin as major metabolites. Among extract and fractions, EAE was found more effective to <i>A. craccivora</i> (lethal dose to kill 50% of test insect <i>i. e</i>., LD<sub>50</sub> = 1.84 µL/nymph) after 96 h followed by <i>n</i>-hexane fraction (LD<sub>50</sub> = 2.22 µL/insect). In <i>P. lilacinus</i>, <i>n</i>-hexane fraction displayed highest toxicity (LD<sub>50</sub> = 1.46 µL/crawler) followed by ethyl acetate and <i>n</i>-butanol fraction (LD<sub>50</sub> = 2.01–2.29 µL/crawler). All combinations of the EAE and fractions exhibited synergetic action. Amongst compounds, linolenic acid was found superior to <i>A. craccivora</i> (LD<sub>50</sub> = 0.59 µL/nymph) and <i>P. lilacinus</i> (LD<sub>50</sub> = 0.99 µL/crawler). EAE and its fractions also showed significant reproductive inhibition and deterrence to target pests. Further, EAE significantly inhibited in vivo enzyme acetylcholinesterase (AChE), glutathione-S-transferase (GST), and mixed function oxidase (MFO) in <i>A. craccivora</i> after 24 and 48 h. In <i>P. lilacinus</i>, only GST showed inhibition but AChE and carboxylesterase (CES 1) were induced after 24 h. SEM study revealed notable aberrations in the structure of the peritoneum, setae, and thoracic legs of <i>A. craccivora</i> after ingestion of EAE. Under greenhouse conditions, the higher dose of <i>R. officinalis</i> EAE (20 g/L) reported higher reduction of <i>A. craccivora</i> on leaf (82.28 to 89.36%) and twigs (70.68 to 85.72%) of cowpea after 3, 5 and 7 days of second spray.</p><h3>Conclusion</h3><p>Based on our greenhouse study results, EAE of <i>R. officinalis</i> may be recommended for the control of <i>A. craccivora</i> in crop plants.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00761-7","citationCount":"0","resultStr":"{\"title\":\"Metabolic profiling and insecticidal activities of Rosmarinus officinalis L. for the management of Aphis craccivora Koch and Planococcus lilacinus Cockerell\",\"authors\":\"Urvashi Kashyap,&nbsp;S. G. Eswara Reddy\",\"doi\":\"10.1186/s40538-025-00761-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Sucking insects are major threat to agricultural and horticultural crops. Indiscriminate application of chemical insecticides for the control of pests leads to the development of resistance, harmful to non-target organisms, consumers’ health, the environment, etc. Therefore, botanical insecticides are alternate to synthetic pesticides for the control of sucking pests. In the present investigation, chemical constituents, metabolic profile, and insecticidal activities of <i>Rosmarinus officinalis</i> L. (Lamiaceae) ethanolic aqueous extract (EAE), fractions and compounds were screened against <i>Aphis craccivora</i> Koch (Hemiptera: Aphididae) and <i>Planococcus lilacinus</i> Cockerell (Hemiptera: Pseudococcidae)<i>.</i></p><h3>Results</h3><p>Gas chromatography (GC) and gas chromatography–mass spectrometry (GC–MS) analysis showed that linolenic acid (24.97%), 1,8-cineole (14.26%), myrcene (10.67%), hexadecenoic acid (9.91%), and camphene (7.12%) were the major constituents in the <i>n</i>-hexane fraction. UHPLC–ESI-QTOF-IMS analysis of ethanolic aqueous extract (EAE) showed the presence of palmitoleic acid, 4-ethoxy ethyl benzoate, 7-methylrosmanol, and diosmin as major metabolites. Among extract and fractions, EAE was found more effective to <i>A. craccivora</i> (lethal dose to kill 50% of test insect <i>i. e</i>., LD<sub>50</sub> = 1.84 µL/nymph) after 96 h followed by <i>n</i>-hexane fraction (LD<sub>50</sub> = 2.22 µL/insect). In <i>P. lilacinus</i>, <i>n</i>-hexane fraction displayed highest toxicity (LD<sub>50</sub> = 1.46 µL/crawler) followed by ethyl acetate and <i>n</i>-butanol fraction (LD<sub>50</sub> = 2.01–2.29 µL/crawler). All combinations of the EAE and fractions exhibited synergetic action. Amongst compounds, linolenic acid was found superior to <i>A. craccivora</i> (LD<sub>50</sub> = 0.59 µL/nymph) and <i>P. lilacinus</i> (LD<sub>50</sub> = 0.99 µL/crawler). EAE and its fractions also showed significant reproductive inhibition and deterrence to target pests. Further, EAE significantly inhibited in vivo enzyme acetylcholinesterase (AChE), glutathione-S-transferase (GST), and mixed function oxidase (MFO) in <i>A. craccivora</i> after 24 and 48 h. In <i>P. lilacinus</i>, only GST showed inhibition but AChE and carboxylesterase (CES 1) were induced after 24 h. SEM study revealed notable aberrations in the structure of the peritoneum, setae, and thoracic legs of <i>A. craccivora</i> after ingestion of EAE. Under greenhouse conditions, the higher dose of <i>R. officinalis</i> EAE (20 g/L) reported higher reduction of <i>A. craccivora</i> on leaf (82.28 to 89.36%) and twigs (70.68 to 85.72%) of cowpea after 3, 5 and 7 days of second spray.</p><h3>Conclusion</h3><p>Based on our greenhouse study results, EAE of <i>R. officinalis</i> may be recommended for the control of <i>A. craccivora</i> in crop plants.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":512,\"journal\":{\"name\":\"Chemical and Biological Technologies in Agriculture\",\"volume\":\"12 1\",\"pages\":\"\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2025-04-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00761-7\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical and Biological Technologies in Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40538-025-00761-7\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-025-00761-7","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

吸虫是农业和园艺作物的主要威胁。为了控制害虫而不加选择地使用化学杀虫剂会导致抗药性的产生,对非目标生物有害,对消费者的健康、环境等有害。因此,植物性杀虫剂可替代合成杀虫剂防治吸虫。研究了迷香草(Lamiaceae)乙醇水提物(EAE)的化学成分、代谢谱和对克拉科蚜虫(Aphis craccivora Koch)和淡紫色小球虫(Planococcus lilacinus Cockerell)的杀虫活性。结果气相色谱(GC)和气相色谱-质谱(GC - ms)分析表明,亚麻酸(24.97%)、1,8-桉树脑(14.26%)、月桂烯(10.67%)、十六烯酸(9.91%)和莰烯(7.12%)是正己烷馏分的主要成分。乙醇水提物(EAE)的UHPLC-ESI-QTOF-IMS分析表明,其主要代谢产物为棕榈油酸、4-乙氧基苯甲酸乙酯、7-甲基迭香醇和二恶星明。在提取物和馏分中,EAE在96 h后的杀伤效果最好(致死剂量为50%,即LD50 = 1.84µL/虫),其次是正己烷馏分(LD50 = 2.22µL/虫)。丁香正己烷部分毒性最强(LD50 = 1.46µL/虫),其次是乙酸乙酯和正丁醇部分(LD50 = 2.01 ~ 2.29µL/虫)。EAE与各组分的组合均表现出协同作用。其中,亚麻酸的LD50值为0.59µL/虫,而丁香的LD50值为0.99µL/虫。EAE及其组分对目标害虫也有显著的繁殖抑制和威慑作用。此外,EAE在24 h和48 h后显著抑制豚鼠体内酶乙酰胆碱酯酶(AChE)、谷胱甘肽- s转移酶(GST)和混合功能氧化酶(MFO)。在丁香花中,只有GST对AChE和羧酸酯酶(CES 1)有抑制作用,24 h后对AChE和羧酸酯酶(CES 1)有诱导作用。扫描电镜研究显示,EAE摄入后,豚鼠腹膜、刚毛和胸腿的结构发生了明显的畸变。在温室条件下,高剂量的officinalis EAE (20 g/L)在第二次喷施3、5和7 d后,对豇豆叶片和枝条的抑制率分别为82.28 ~ 89.36%和70.68 ~ 85.72%。结论基于温室研究结果,可推荐利用officinalis EAE对作物植物中的裂口蚜进行防治。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolic profiling and insecticidal activities of Rosmarinus officinalis L. for the management of Aphis craccivora Koch and Planococcus lilacinus Cockerell

Background

Sucking insects are major threat to agricultural and horticultural crops. Indiscriminate application of chemical insecticides for the control of pests leads to the development of resistance, harmful to non-target organisms, consumers’ health, the environment, etc. Therefore, botanical insecticides are alternate to synthetic pesticides for the control of sucking pests. In the present investigation, chemical constituents, metabolic profile, and insecticidal activities of Rosmarinus officinalis L. (Lamiaceae) ethanolic aqueous extract (EAE), fractions and compounds were screened against Aphis craccivora Koch (Hemiptera: Aphididae) and Planococcus lilacinus Cockerell (Hemiptera: Pseudococcidae).

Results

Gas chromatography (GC) and gas chromatography–mass spectrometry (GC–MS) analysis showed that linolenic acid (24.97%), 1,8-cineole (14.26%), myrcene (10.67%), hexadecenoic acid (9.91%), and camphene (7.12%) were the major constituents in the n-hexane fraction. UHPLC–ESI-QTOF-IMS analysis of ethanolic aqueous extract (EAE) showed the presence of palmitoleic acid, 4-ethoxy ethyl benzoate, 7-methylrosmanol, and diosmin as major metabolites. Among extract and fractions, EAE was found more effective to A. craccivora (lethal dose to kill 50% of test insect i. e., LD50 = 1.84 µL/nymph) after 96 h followed by n-hexane fraction (LD50 = 2.22 µL/insect). In P. lilacinus, n-hexane fraction displayed highest toxicity (LD50 = 1.46 µL/crawler) followed by ethyl acetate and n-butanol fraction (LD50 = 2.01–2.29 µL/crawler). All combinations of the EAE and fractions exhibited synergetic action. Amongst compounds, linolenic acid was found superior to A. craccivora (LD50 = 0.59 µL/nymph) and P. lilacinus (LD50 = 0.99 µL/crawler). EAE and its fractions also showed significant reproductive inhibition and deterrence to target pests. Further, EAE significantly inhibited in vivo enzyme acetylcholinesterase (AChE), glutathione-S-transferase (GST), and mixed function oxidase (MFO) in A. craccivora after 24 and 48 h. In P. lilacinus, only GST showed inhibition but AChE and carboxylesterase (CES 1) were induced after 24 h. SEM study revealed notable aberrations in the structure of the peritoneum, setae, and thoracic legs of A. craccivora after ingestion of EAE. Under greenhouse conditions, the higher dose of R. officinalis EAE (20 g/L) reported higher reduction of A. craccivora on leaf (82.28 to 89.36%) and twigs (70.68 to 85.72%) of cowpea after 3, 5 and 7 days of second spray.

Conclusion

Based on our greenhouse study results, EAE of R. officinalis may be recommended for the control of A. craccivora in crop plants.

Graphical Abstract

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Chemical and Biological Technologies in Agriculture
Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.80
自引率
3.00%
发文量
83
审稿时长
15 weeks
期刊介绍: Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信